Catecholamines ; blood ; Heart Rate ; Humans ; Water Sports ; physiology

Blood Chemical Analysis ; Humans ; Water Sports ; physiology

ObjectiveTo explore the value of color Doppler echocardiography in diagnosing apical hypertrophic cardiomyopathy( apical hypertrophic cardiomyopathy, AHCM). MethodsRetrospective analysis of 28 cases with apical hypertrophic cardiomyopathy by echocardiography images was carried out. Results28 cases AHCM patients,apical myocardial thickness 17 ～29mm,5 cases of simple apical myocardial thickening;10 cases of ventricular apex、left ventricular myocardial wall thickening in the apical segment;6 cases of ventricular apex、left ventricular myocardial thickening of apical segments of inferior;6 cases of Ventricular apex,left ventricular free wall thickening of apical myocardial; 1 case of ventricular apex、right ventricular free wall thickening of apical myocardial. All patients were diagnosed. ConclusionColor Doppler echocardiograph was an effective method for diagnosis of apical hypertrophic cardiomyopathy.

Objective To evaluate the influence of cytochrome P450 (CYP2C9 and CYP4F2) polymorphisms on anticoagulant intensity of warfarin after cardiac valve replacement.Methods A total of 136 patients tak ing warfarin after cardiac valve replacement were identified and classified into 4 groups:CYP2C9 wild type group (CYP2C9*1*1),CYP2C9 mutated type group (CYP2C9*3),CYP4F2 rs2108622 wild type group (CC) and CYP4F2 rs2108622 mutated type group (CT or TT).The patients' baseline data,initial dose of warfarin and base INR measurement resuhs were recorded and then the follow-up was conducted.The initial administration of warfarin to INR standard time for the first time,total amount of warfarin and the average daily amount were recorded.Results Patients carrying CYP2C9* 1* 1 had increased time to reach INR target value for the first time (P ＜ 0.05);and the total warfarin doses and average daily dose when INR reached target value were higher than those carrying CYP2C9*3 (P ＜ 0.05).When compared with those in two wild type groups,patients carrying CYP2C9 and CYP4F2 rs2108622 mutated type needed the shortest time when INR reached target value for the first time,and the total warfarin doses and average daily dose when INR first reached target value was the lowest,which showed significant difference (P ＜ 0.05).And when compared with CYP2C9 mutated type group,the INR average time to reach the first target was shortened and the total warfarin dose of patients carrying CYP2C9 and CYP4F2 rs2108622 mutated type was lower (P ＜ 0.05).Conclusion The gene polymorphisms of CYP2C9 and CYP4F2 are significant hereditary factors influencing warfarin dose.Detection of CYP2C9 and CYP4F2 genotypes prior to medication and predicating warfarin dosage may result in lower incidence of over-anticoagulation and reduce the dosage-adjusting time of warfarin.

ObjectiveTo evaluate the efficacy and safety of hydroxychloroquine(HCQ) in pregnant patients with systemic lupus erythematosus (SLE).Methods Twenty-four pregnant patients with SLE treated with HCQ during pregnancy from May,2006 to February,2011 were studied retrospectively.All babies were followed up during early infancy for growth development.ResultsOf them,22 patients were treated with HCQ throughout the whole pregnancy with no lupus flare occurred in 21 patients (95.4％),while temporary discontinuation of HCQ precipitated a flare of disease in two patients.Three patients ( 12.5％ ) had premature delivery,and pregnancy induced hypertension happened in 3 patients (12.5％ ).No congenital abnormalities occurred and mean follow-up of 26 months ( range 1 - 47 months) revealed no abnormalities in these children.Conclusion Our findings reinforce the safety of HCQ therapy during pregancy and HCQ should probably be maintained throughout the pregancy in patients with SLE.

AIM To establish an efficient and convenient system for expressing functional proteins. METHODS Xenopus leavis females were injected with HCG, eggs were harvested next morning and dejellied by 2% cysteine, from which the Xenopus leavis egg cell free translating system was prepared. Through subcloning technique, TFAR19 gene was cloned to pBluescript SK plasmid which was linearized by enzyme, then transcript and capped in vitro to synthesis the cRNA of TFAR19. RESULTS This cRNA was translated in freshly prepared cell free translating system, the product was identified by Western Blot. CONCLUSION The results showed that this translating system could efficiently translate TFAR19 cRNA and be a very useful translating tool.

Acupuncture Points ; Adult ; Drugs, Chinese Herbal ; administration & dosage ; Ear ; anatomy & histology ; Female ; Humans ; Melanosis ; drug therapy ; Middle Aged ; Young Adult

SCCmec Ⅳ.The strains with SCCmec Ⅱ and SCCmec Ⅲ were multi-resistant and their resistance rates were higher than SCCmec Ⅳ (P

Objective:To study the wound healing effects of the extracts of Shengji Huayu Fang with different solvents and demon-strate their important role in wound healing for deep second degree burn wound. Methods:Sixty Sprague-Dawley rats were randomly di-vidied into six groups(ten rats in each group): the control group (saline), the positive control group (asiaticoside group), Shengji Huayu Fang group respectively with petroleum ether, chloroform, ethyl acetate and n-butanol as the extraction solvent. The extracts of Shengji Huayu Fang with different solvents were applied in the rats with deep second degree burn wound, and the wound healing was e-valuated by the healing rate judged by naked eyes and computer image analysis, DNA cell cycle analysis using a flow cytometry, patho-logical reports and the degree of re-epithelialization studied by the other methods. Results:The mean healing time of the chloroform ex-tracts group [(15. 67 ± 1. 12)d] was much shorter than that of the control group [(22. 87 ± 1. 01)d, P<0. 01]. The hydroxyproline content and the percentage of S-phase cells in wound tissue in the chloroform extracts group was obviously higher than those in the con-trol group (P<0. 01). Conclusion:The present study indicates that topical application of chloroform extracts of Shengji Huayu Fang is beneficial to burn wound healing, and the chloroform extracts of Shengji Huayu Fang is the main bioactive fraction.