Objective To study the relationship between the number of myeloid-derived suppressor cells (MDSCs) in peripheral blood and prognosis in patients with peripheral T-cell lymphoma (PTCL). Methods Twenty-three patients with PTCL were selected in this study and 14 healthy persons were used as the normal control. The proportion of MDSCs/mononu?clear in peripheral blood was detected by flow cytometry. The correlation between the number of MDSCs and the clinical character and prognosis in patients with PTCL was analyzed. Results The proportion of MDSCs in peripheral blood was sig?nificantly higher in patients with PTCL than that of healthy subjects [(4.59±1.41)%vs (1.36±0.59)%, P<0.01]. The number of MDSCs in peripheral blood was significantly decreased after chemotherapy compared with that before treatment [( 1.29 ± 2.95)%, P<0.05)]. There were significant differences in MDSCs expressions in patients between different extranodal involve?ment, bone marrow involvement, clinical stage and IPI score (P<0.05). Conclusion The higher expression of MDSCs may be related to the progression in patients with PTCL.

Objective] To explore the effect of Biejiajian oral Liquid (BOL) on the level of angiotensinⅡ(AngⅡ) and angiotensin(1-7) [Ang(1-7)],and ratio of AngⅡ/Ang(1-7) in liver fibrosis rats. [Methods] Randomly divide male Wistar rats into blank group, model group, BOL groups of high dose and low dose, 10 in each. The model rats were injected with inactivated pig serum abdominally, 0.5mL for each, 2 times/w, for successive 8w. Other rats were injected with the same dosage and period of normal saline. After modeling, administer BOL 10g·kg-1 to low-dose groups rats, and 20g·kg-1 to high-dose group rats; give equal distilled water to blank and model groups, for successive 5 weeks of intragastric administration. The liver histopathology was examined by hematoxylin-eosin(HE) and Masson trichrome staining. Liver and spleen index were calculated. Levels of alanine transaminase(ALT) and aspartate aminotransferase(AST) in serum were assayed by automatic biochemistry detection instrument. Contents of hyaluronic acid(HA), laminin(LN), typeⅢprocollagen(PcⅢ) and type IV collagen(Ⅳ.C) in serum were detected by radioimmunoassay. AngⅡand Ang(1-7) levels were determined by enzyme linked immunosorbent assay. [Results] Compared with model group, the histological injury of liver in rats of BOL groups was relieved; serum levels of AST, ALT, HA, LN, PCⅢ and Ⅳ.C, and the content of AngⅡ in liver homogenate were decreased significantly than that of the model group. Ang(1-7) level was further increased, AngⅡ/Ang(1-7) ratio was decreased obviously in BOL groups. [Conclusion] BOL showed the protective function for hepatic fibrosis, the mechanisms of which may be associated with improvement of Ang(1-7) level, decrease of AngⅡlevel and the ratio of AngⅡand Ang(1-7).

Objective To evaluate the timing of postoperative parenteral nutrition support for gastrointestinal tumor patients with a definite nutritional risk.Methods Using a randomized control clinical research methods,80 gastrointestinal cancer patients were randomly divided into study group (40 cases) and control group (40 cases).In the study group parenteral nutrition support started on postoperative day 2,while in the control group it started on postoperative day 1.Flatus passing,the rate of infectious complications,the rate of systemic inflammatory response syndrome,postoperative nutrition support related expenses,and the duration of postoperative hospital stay were recorded.Results There was no significant differences between the two groups (P ＞ 0.05) in age,sex,body mass index,use of glutamine,disease and type of surgery.Compared with the control group,patients in the study group had earlier postoperative flatus passing [(3.33± 1.10) d vs.(4.03 ± 1.51) d,P =0.020],less systemic inflammation response syndrome (8/40 vs.17/40,P =0.030),shorter postoperative hospital stay [(3.33±1.10) d vs.(4.03±1.51) d,P=0.020] and lower nutrition support costs [(2144.49 ±1210.96) RMB vs.(2915.99 ± 1615.68) RMB,P =0.018].There was no significant difference in postoperative nutrition index,peristalsis recovery time and infectious complications between the two groups (all P ＞ 0.05).Conclusions In patients undergoing surgery for GI tumors,parenteral nutrition support started on post-op day 2 promotes the recovery of gastrointestinal function,while decreasing medical cost.

Three hundred and thirty eight patients with type 2 diabetes mellitus( DM) admitted from January 2006 to June 2009 and 50 healthy subjects (control group) were enrolled in the study. Platelet glycoprotein PAC-1 and CD62P levels were measured in both groups. Compared with control group, plasma PAC-1 and CD62P levels of DM patients increased significantly (P ＜0.01). Patients were divided into three groups according to their urinary albumin excretion rate ( UAER) . PAC-1 and CD62P levels in macroalbuminuria group (UAER≥200μg/min) were significantly higher than those in normoalbuminuria group( UAER <20μg/min) and microalbuminuria group (20μg/min≤UAER ＜ 200μg/min) (both P＜ 0.05). The results indicate that plasma levels of PAC-1 and CD62P, which reflect platelet activation, are the independent risk factors for diabetic nephropathy.

Humans ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; metabolism ; Proto-Oncogene Proteins c-akt ; metabolism ; Signal Transduction

OBJECTIVE:To investigate the stability of injectable pantoprazole sodium in 4 kinds of infusions.METHODS: A HPLC method was adopted in this determination, the content change of injectable pantoprazole sodium was determined within 4 hours' mixing with 4 kinds of infusions and its change of color was observed; meanwhile the effects of pH value, potassium,magnesium,calcium ions on the stability of injectable pantoprazole sodium were investigated.RESULTS:No obvious changes were noted in terms of contents, appearances, pH value, UV - absorption maximum wavelength for the injectable pantoprazole sodium solution within 4 hours either in 4 kinds of common infusions or in potassium,magnesium,calcium ions water solutions.It was extremely unstable when pH value of pantoprazole sodium solution was lower than 7.0;when its pH value was 7.0, its color became yellowish yet without obvious loss of content; it is stable within 4h when its pH value was above 8.0.CONCLUSIONS:Injectable pantoprazole sodium remains stable within 4 hours either in 4 kinds of common infusions or in potassium, magnesium, calcium ions water solutions, the pH value has a great influence on the stability of pantoprazole sodium solution.

Objective To explore the role of P38 mitogen-activated protein kinase (P38 MAPK) in the development of cerebral hypoxic preconditioning. Methods Healthy male BALB/C mice weighted as18～20 g were randomly divided into 7 groups as follows: normoxic control (H0), early (H1～H4) and delayed (H5 and H6) hypoxic preconditioned mice groups. SDS-PAGE, Western blot and Gel Doc imagine systems were applied to quantitatively analyze the level of P38 MAPK phosphorylation and protein expression in the brain of mice. Results The phosphorylation levels of P38 MAPK increased in cortex, hippocampus and hypothalamus of mice in both early (H1～H4) and delayed (H5 and H6) hypoxic preconditioned groups, and the statistic significance (P

Objective To investigate the clinical meaning and feasibility of transient evoked otoacoustic emission (TEOAE) in China rural primary school children hearing screening.Methods TEOAE was recorded with Madsen Celesta 503 in 317 (634 ears) primary school children,then the results were compared with gold standard test group,including otoscopy, pure tone audiometry and tympanogram.Results In 317 (634 ears) pupils, the presence of TEOAE was 94.64% (600/634); the presence of gold standard was 94.48% (599/634). The consistency rate was (96.69?0.71)%; sensitivity (Sen) was 0.69?0.078;specificity (Spe)=0.98?0.0052, the false positive rate (FPR), that was ?, was 0.31; ? = 0.02; Youden's index J = 0.67?0.078, LR_+=34.5, LR_-=0.32, PV_+=0.71(71%),PV_-=0.98(98.%),AZ=0.8.Conclusion The TEOAE test is simple, quick, harmless, sensitive and objective. It can be used in hearing screening program in rural primary school children.

Objective To study whether stilnox intervention could prove the prognosis for subjective tinnitus patients combined with sleep disorders.Methods By random and double-blind method,68 tinnitus patients with sleep disorders were randomly divided into the treatment group and control group,with 34 cases in each group.The patients in the control group received ginaton and methycobal oral administration.On the basis of this,the patients in the treatment group received Stilnox oral administration before sleep.All objects of study were evaluated using Tinnitus handicap inventory (THI)and Pittsburgh sleep quality index (PSQI)two weeks before and weeks after treatment.Results The tinnitus disability in the treatment group showed statistical differences before and after treatment (t=8.810,<0.05).The levels of sleep disorders in the treatment group showed statistical differences after treatment (t=20.446,<0.05).The tinnitus disability in the control group showed statistical differences after treatment(t=6.900,<0.05).The sleep disorders in the control group showed statistical differences before and af-ter treatment(t=4.631,<0.05).The tinnitus disability (t=2.313,<0.05)and sleep disorders(t=7.906,<0. 05)after treatment showed statistical differences between the treatment group and control group.Conclusion Stil-nox intervention therapy could improve the prognosis of subjective tinnitus patients with sleep disorders.

BACKGROUND:It is necessary to carry out multiple operations to remove the scar in patients with large area of scar, and whether the scar tissue can be recycled has become the focus of the study. OBJECTIVE:To compare the tissue structure, biomechanical properties and biocompatibility of the acel ular dermal matrix of mature scar tissue and normal skin. METHODS:The acel ular dermal matrix was prepared from the human mature scar tissue and the normal skin around the scar. Subsequently, histological and scanning electron microscope observations were performed, and biomechanical properties were detected using universal tensile testing machine. Then, the acel ular dermal matrix from mature scar tissue and normal skin was co-cultured with fibroblasts for 10 days, respectively, and the cel growth curve was drawn. Additional y, the acel ular dermal matrix from mature scar tissue and normal skin was subcutaneously implanted into the dorsal tissue of Sprague-Dawley rats, respectively and histological observation was conducted at 4, 8 and 12 weeks after implantation. RESULTS AND CONCLUSION:There were many gaps but no cel ular components in the acel ular dermal matrix, in both two groups. Col agen fibers of the acel ular dermal matrix derived from mature scar were looser than that of the normal skin, and arranged slightly irregularly;the biomechanical properties of the acel ular dermal matrix derived from mature scar were similar to that of the normal skin, which exhibited appropriate flexibility and strength. There was no significant difference in the growth state of the two kinds of acel ular dermal matrix, and the growth curve was basical y consistent. After 4 weeks of implantation, more inflammatory cel s infiltration could be found in the mature scar group, and in contrast, only a few inflammatory cel s infiltration appeared in the normal skin group, These inflammatory reactions disappeared with time in both two groups. Besides, col agen fibers arranged in neat, and smal vessels grew into the implants in both two groups. In conclusion, the tissue structure, biomechanical properties and biocompatibility of the acel ular dermal matrix derived from scar tissue are almost consistent with those of the human normal skin.