Objective To study the effect of TanshinoneⅡA(TSN) on vascular smooth muscle cells(VSMCs) induced by high glucose and its relationship with MAPK signal pathway.Methods After the establishment of the model of diabetic rats,the aortic of rats was isolated and cultured.DNA synthesis was analyzed by ELISA of BrdU.The activity of MAPK was measured by liquid scintillation counting.Results TSN inhibited the proliferation and DNA synthesis of vascular smooth muscle cells from diabetic rats in dose-dependent manner.TSN at the dosage of 0.5 mg/L significantly decreased the activity of MAPK as well as of U0126.Conclusion The effect of TSN on the proliferation and DNA synthesis of vascular smooth muscle cells from diabetic rats is potentially mediated by inhibition of MAPK.

[Objective] Summing up the specific application of the three stages therapy of Professor XU Li in the treatment of cancer.[Methods]By copying the prescription and consulting the medical books of Professor XU, learning his clinical experience in treatment of cancer,summing up the core thinking of the three stages therapy and the experience of Chinese medicinal plant. [Result]By summing up the three stages therapy(assisting ,maintaining and palliative treatment),the special herb and attaching importance to develop the XIAN TIAN and HOU TIAN in the treatment of cancer ,it can improve radiation and chemotherapy therapeutic efficacy and resistance to transfer the maintenance treatment of recurrence ,improve the quality of life and prolong survival time of palliative care.By the guidance of the three stages therapy,smoothly finish the postoperative radiotherapy of nasopharyngeal carcinoma,successfully reverse the precancerous lesions of chronic atrophic gastritis with intestinal metaplasia,the two typical medical cases have significant curative effect. [Conclusion] Taking the three stages therapy in the process of the treatment of the tumor and precancerous lesions ,it can get good clinical effect,it is worth spreading in clinic.

Objective To explore the application value of the diffusion-weighted imaging (DWI)and dynamic contrast-enhanced MRI in detection of peripheral zone of hepatic alveolar echinococcosis (HAE).Methods Twenty patients with HAE underwent abdominal plain scan,DWI and dynamic enhanced MRI.The ADC values and enhanced ratio of the central necrosis,solid component,peripheral zone and normal liver parenchyma were measured at a workstation,and further were analyzed and compared.Results 32 lesions were detected in 20 patients with HAE.Two groups were divided according to the lesion with central necrosis (group A)or not (group B).In group A, the ADC values of the solid part and normal liver parenchyma were (1.52±0.30)×10 -3 mm2/s and (1.22±0.30)×10 -3 mm2/s. Meanwhile,in group B those were (1.50±0.25)×10-3 mm2/s and (1.03±0.28)×10-3 mm2/s,exhibiting no statistical differences between the two groups.In group A and B,the ADC values of the peripheral zone of lesion were 1.34(1.10,1.61)×10 -3 mm2/s and 0.96 (0.86,1.22)×10 -3 mm2/s,exhibiting statistical differences (Z =2.867,P =0.004);meanwhile,the enhanced ratio were 1.70± 0.36 and 1.58±0.30,exhibiting no statistical difference.No correlation between ADC values and enhanced ratio was found in the peripheral zone.Conclusion DWI at 3.0T can display visually the HAE,and the ADC value may reflect the DWI features of periph-eral zone.The ADC values in combination with enhanced ratio may help for the diagnosis.

Objective: Digital images processing is widely applied in the fields of biology and medicine, and some achievements have been gained by this technique. But there is few reports on measure the parameters of morphology of pathology from the patients which were threated by ovulation induction, by computer processing techniques. The work shows it has significant value in clinical diagnosis and reproductive medicine at present and in the future. Methods: Write the programmeces by Image Processing Toolbox in MATLAB. The original pathology images are preprocessed by 4 steps: step 1: read imagejstep 2: imopen the curve background brightness; step 3: adjust the background; step 4: adjust the contrast. Then segmentation image by 4 steps; step 1: read image; step 2: estimation the image; step 3: remove the noise; step 4: find the boundaries. The end, measure the parameters of morphology. Results: The pathology was studied percase for their area, perimeter, maximum diameter, equivalent diameter and metric. Conclusions: Image Processing Toolbox is useful to measure the parameters of morphology of pathology.

Objective:To compare the content differences of 18 amino acids in plancenta histolysate determined by pre-column de-rivatization HPLC and post-column derivatization cation-exchange chromatography ( AARO) . Methods: The HPLC method was per-formed on a C18 column and 2, 4-dinitro chlorobenzene ( DNCB) was used for pre-column derivatization, and then the determination was carried out after adding 0. 1 mol·L-1 borax buffer (pH=9. 1), and the AARO method was used for the direct determination with a strong acid cation-exchange chromatographic column and post-column derivatization. Results: The RSD for reproducibility of the AARO method was 1. 84%-0. 91%, while that of the HPLC method was 1. 87%-1. 04%. Conclusion:Both AARO method and HPLC method can be used for the quantitative determination of 18 amino acids in plancenta histolysate with the similar results. However, pre-column derivatization HPLC method may cause incomplete derivatization and instable derivatives.

After a description of the development in Chinese minority medicine and medical documents, the class (R29) for Chinese minority medical documents offered in Chinese Library Book Classification,5th edition was analyzed. The limitations for the classification of Chinese minority medical documents offered in Chinese Library Book Classifi-cation,5th editionwere pointed out, including no classes for the modern researches and subjects in Chinese minority medical documents , with strategies put forward for adding classes in R29 for Chinese minority medical documents in Chinese Library Book Classification,5th edition.

Objective To detect serum concentrations of Dickkopf-1(DKK-1) and soluble receptor activator of nuclear factor-κB ligand (sRANKL) in patients with multiple myeloma (MM) and to investigate its clinical significance. Methods Serum DKK-1, sRANKL, osteoporotegerin(OPG) and tartrate-resistant acid phosphatase 5b (TRACP-5b) levels were quantified in 30 newly diagnosed MM patients and 20 healthy control subjects by using sandwich ELISA. Results The serum DKK-1, sRANKL,OPG and TRACP-5b levels were significantly higher than those in the healthy controls (42.96 μg/L vs 5.33 μg/L, 1.83 pmol/Lvs 0. 79 pmol/L, 1799. 30 pmol/L vs 822.40 pmol/L, 5.81 U/L vs 0. 28 U/L, respectively; all P＜0. 05). Serum levels of DKK-1 were positively correlated with sRANKL and TRACP-5b, respectively.Serum concentrations of DKK-1 and sRANKL were significantly elevated in stage Ⅲvs stages Ⅰ and Ⅱaccording to International Staging System (ISS) (46. 33 μg/L vs 37.91 μg/L, 2.26 pmol/L vs 1.19pmol/L, respectively, all P ＜0.05). Serum concentrations of DKK-1 , sRANKL and TRACP-5b were significantly higher in patients with more than 3 lytic bone lesions than those with only 1-3 lytic bone lesions (46. 30 μg/L vs 31.98 μg/L, 2. 18 pmol/L vs 0. 69 pmol/L, 6.02 U/L vs 5. 13 U/L, all P ＜ 0.05).Conclusions MM patients have increased serum DKK-1, sRANKL, OPG and TRACP-5b levels as compared with the healthy controls. Serum concentrations of DKK-1 and sRANKL have close relationship with MM stage and lytic bone disease.

By literature research and questionnaire, the paper analyzed development mode and status of scientific researching institutions of Traditional Chinese Medicine in other countries, discussed necessity and realistic meaning of establishment of the standards, and advocated the standards must be followed the rule of standing out characteristic of traditional Chinese medicine, making use of modern scientific technology and instrument, emphasizing commonness, classifying instruction and getting hold of major point.

Objective:To compare and analyze clinical features between patients with early and non‐early acute coro‐nary syndrome (ACS) .Methods :A total of 120 ACS patients were selected ,including 71 patients with early ACS (early ACS group ,onset time≤12h) and 49 patients with non‐early ACS (non‐early ACS group ,onset time >12h) . Risk factors ,clinical features ,mortality ,incidence rates of myocardial infarction ,rehospitalization and major ad‐verse cardiovascular events (MACE) were compared and analyzed between two groups .Results:There were no sig‐nificant difference in percentage of each ACS risk factor between two groups , P>0.05. Compared with non‐early ACS group ,there was significant reduction in incidence rate of heart failure (73.5% vs .54.9% ) ,significant rise in incidence rates of obvious chest pain (32.7% vs .73.2% ) and shock (46.9% vs .62.0% ) in early ACS group (P<0.05 all) ,but there was no significant difference in incidence rate of arrhythmia between two groups (P>0.05) . After one‐year follow‐up , compared with non‐early ACS group , there were significant reduction in mortality (18.4% vs .4.2% ) ,incidence rates of myocardial infarction (18.4% vs .4.2% ) ,rehospitalization (30.6% vs . 14. 1% ) and MACE (38. 8% vs .16. 9% ) in early ACS group , P<0. 05 all .Conclusion:Initial symptom is more ob‐vious ,but related complications are fewer and the prognosis is better in patients with early acute coronary syndrome .

Objective:To explore the polarization of Th1 and Th2 immune response and the changes of transcription factor(T-bet and GATA-3) in BALB/c mice infected with toxoplasma gondii.Methods:Sixty BALB/c mice were infected with toxoplasma tachyzoite(1?104/m,0.5 ml) by intraperitoneal injection and thirty BALB/c mice only received an injection of same amount of normal saline as control.After injection,two mice of infection group and one mouse of control group were killed in odd number days.Then,the serum IFN-? and IL-4 were measured by ELISA,and the mRNA expression of T-bet and GATA-3 in mice spleens were detected by Real-Time RT-PCR.Results:In the infection group of mice,the IFN-? was remarkable increased at the fourth day and kept the peak from the 5th day to 7th day.Then,it decreased to normal level at 9th day postinjection;but IL-4 was remarkable increased at the 8th day and reached to the peak at 9th day.Then it decreased to normal level at 15th day postinjection.The mRNA of T-bet expression was increased at the third day and kept the peak from the 5th day to 7th day.Then,it decreased to normal level at 9th day postinjection.The mRNA of GATA-3 expression was increased at the 7th day and rised to the peak at 11th day.Then,it decreased to normal level at 13th day postinjection.There were not obvious changes of IFN-? and IL-4 or T-bet and GATA-3 in normal mice.Conclusion:The Th1 immune response is dominant in acute infection when mice are infected by toxoplasma.Then it biases toward Th2 response at 9th day to 13th day postinfection.The shift of Th1/Th2 polarization influences the finale of toxoplasma infection.