AIM: To observe the pathological role of 3-nitrotynosine (3-NT) on Escherichia coli LPS-induced vascular hyporeactivity in rats and the therapeutic effect of antioxidants. METHODS: Forty male SD rats weighting from 200 g to 250 g were randomly divided into four groups: the control group (n=10); LPS shock group (n=10); uric acid-treated group (n=10); melatonin-treated group (n=10). 6 h after LPS shock, phenylephrine (0.5-2.5 ?g?kg -1) was applied intravenously to all groups and the percentage increase in MAP was detected, respectively. The concentration-response curve of aorta rings from all groups rats were obtained by cumulative addition of phenylephrine (PE), and PE E_ max, EC_ 50 were calculated. The concentrations of plasma malondialdehyde (MDA), nitrate/nitrite and 3-NT were assayed in all groups 6 h after LPS shock. RESULTS: The MAP level induced by PE significantly decreased to 54.60% in LPS shock rats compared with the control (P

Objective To explore the causes of misdiagnosis through clinical data analysis of primary Sjogren syndrome ( PSS) patients misdiagnosed as primary hypothyroidism disease.Methods The clinical data of 34 cases with primary Sjogren syndrome ( pSS ) who were misdiagnosed as primary hypothyroidism were retrospectively analyzed.The misdiagnosis reason was analyzed.Results In 34 patients,a total of 14 patients with primary hypothy-roidism could not explain the clinical signs and symptoms, included dry mouth, dry eyes, recurrent parotid gland enlargement,both hands reynolds phenomenon, purpura like rash and joint pain in the lower extremities.A total of 20 patients with primary hypothyroidism could not be explained by an auxiliary examination abnormality, including ESR,IgG,IgM or IgA increased,white blood cells and platelets,low blood potassium,pulmonary CT suggest interstitial lung disease,ANA (+) (>1:40),RF(+)(>1:20).Conclusion pSS misdiagnosis for hypothyroidism is not uncommon.Department of Endocrinology physicians should pay special attention to hypothyroidism can not explain the symptoms and auxiliary examination abnormal, make the necessary differential diagnosis to reduce the misdiagnosis rate.

Eddy current is one of the most important factors that influence image quality during the process of magnetic resonance imaging. This paper analyzes the reason that eddy current appears and introduces several methods to compensate it. A new method to adjust parameters of eddy current compensation is developed, which proves efficient to enhance image quality through XGY OPER-0.3 MRI system.

It is of essential importance for college students to think creatively. Flexible teaching methods have been adopted during the teaching of medical writing in our university. We inspired, guided, stimulated students to develop their creative thought. The purpose of the article is to develop the creative thought of the students in medical universities.

Objective We aimed to develop a safe, comfortable and effective perineal washer, which prevent perineum wound from infection and improve the healing of perineal wound. Methods The perineal washer was made from high polymer medical material, including two metal catheters, pressing gasbag, medical yoke and dicty blow head. We divided women patients after vagina delivery with perineum wound or after caesarean section into the observation group and the control group randomly with 120 cases in each group from Janurary to October 2007. The control group was given perineal washing by routine washing pot and cotton ball with forceps. The observation group received perineal washing by perineal washer with cleaning fluid and was scrubbed by sponge brash. The elimination of wood swelling, the healing of perineum healing, the urinary infection for patients with indwelling urinary catheterization and patients comfort degree were observed in the two groups. Results Statistical difference existed between the two groups in such aspects, elimination of wound swelling wound healing urinary tract infection and patients comfort degree, P<0.05 or P<0.01. Conclusions Adoption of self designed perineal washer accompanied with sponge brush could completely delete bacteria and inflammatory secretion deep in the vagina and prevent the incidence of infection. It not only could hasten the elimination of wound swelling, alleviate the pain and wound healing, but also was easily developed and portable to use. It provided a new and effective method for perineal washing and is worth clinical application.

Objective To investigate the effective nursing measures of reducing the complications of intratra-cheal suction about mechanical ventilation patients to improve airway nursing quality. Methods 80 patients with ar-tificial air passage mechanical ventilation were divided into Control group (Group one, 40 cases) and Testing group (Group two, 40 cases) randomly. In the control group, suck at regular time after injecting normal saline solution in-to the in-airway (in order to diluted the sputum). There are improvement that nursing measures in testing group. For sample, suck sputums according to demands, choose appropriate sucking sputums tube, control the deepth of sucking sputum tube, adjust the negative pressure based on the deepth of sucking sputum tube, give pure oxygen two to three minutes before and after the suction, strengthen the airway humidfication, lubricate the sucking sputum tube with chlorotetracycline eye ointment, etc. Complications of intratracheal suction were compared in two groups. Results The comparison showed that the complications have a significant statistical difference in sputum obstruction, SpO2 de-scending to 80 percent and below during suction and airway mucous membrane traumatic hemorrhage. (P < 0.01). Conclusion The improved intratracheal suction will decrease the complications obviously like sputum obstruction, SpO2 reducing to 80 percent and below during suction and airway mucous membrane traumatic hemorrhage.

Colorectal cancer (CRC) generally occurs in older patients, however the proportion of young patients with CRC is increasing in recent years, which has drawn lots of scholar′s attention, but their research results were controversial. The current literature from several fields, including clinicopathological characteristic of young CRC patients, the optimal age cutoff between young and elder CRC patients, and the prognosis, early screening and diagnosis in young CRC patients will be reviewed.

Objective:To investigate the effects and mechanism of Ginseng polysaccharides (GPS) on glycosaminoglycan synthesis in rat chondrocytes in vitro.Methods:Chondrocytes isolated from knee joint cartilage of rats were subcultured.After monolayers were established,chondrocytes induced by 20 ng·ml-1 IL-1β were treated with GPS respectively at the concentration of 2,10 and 50 ng ·ml-1for 48 h.The effects of GPS on chondrocytes were analyzed using MTT assay for chondrocyte proliferation and 1,9-dimethylmethylene blue assay for GAG content.mRNA content in xylosyltransferase-Ⅰ(XylT-Ⅰ),galactosyltransferase-Ⅰ(GalT-Ⅰ),galactosyltransferase Ⅱ(GalT-II) and galactose-β-1,3-glucuronosyltransferase Ⅰ(GlcAT-Ⅰ) and that in matrix-degrading enzymes matrix metalloproteinases-3 (MMP-3) and aggrecanase-1and aggrecanase-2 was also detected.Results:GPS had no toxic or proliferating effect on chondrocytes at all observed concentrations.GPS at the concentrations of 10 ng·ml-1 and 50 ng·ml-1 up-regulated GAG synthesis (P<0.01).10 ng·ml-1 GPS enhanced the mRNA expression of XylT-Ⅰ (P<0.01),and 50 ng·ml-1 GPS enhanced the mRNA expression of XylT-Ⅰ,GalT-Ⅰ and GalT-II (P<0.05,P<0.01).GPS did not inhibit the mRNA expression of matrix-degrading enzymes MMP-3 and aggrecanase-1and aggrecanase-2 enhanced by IL-1β.Conclusion:GPS can improve GAG synthesis in IL-1β stimulated chondrocytes in vitro,which is due to the expression promotion of GAG synthesis enzymes without the expression inhibition of matrix-degrading enzymes.

OBJECTIVE:To discuss the characteristics of drug-induced eye impairment.METHODS:206 cases of drug-induced eye impairment reported in internal medical journals published from Jan.1998 to Dec.2005 were analyzed statistically.RESULTS:The drug-induced eye impairment was mostly induced by anti-infective drugs,hormones,nervous system drugs.The impairment was manifested predominantly by glaucoma,keratitis,scleritis;retinopathy,hyphema,xeroma,damage of corneal epithelium and visual disorder etc.CONCLUSION:Clinicians should pay great attention to the drug-induced eye impairment for it can lead to a decline in eyesight or even loss of eyesight.

Objective To explore the relative factors and prognosis of upper gastrointestinal bleeding in patients with hepatic cirrhosis. Methods The clinical data of upper gastrointestinal bleeding of 502 inpatients from January 1990 to September 2004 were statistically analyzed. Results Males and middle-aged patients with hepatic cirrhosis occurred upper gastrointestinal bleeding more frequently, and the most commom causes were vessel rupture of esophage or gastric varices, portal hypertensive gastropathy, and peptic ulcer. The most common complications of upper gastrointestinal bleeding were severe bleeding shock, hepatic encephaopathy, hepatorenal syndrome,etc. Drinking wine and NSAIDs were the most common inducements of upper gastrointestinal bleeding. The efficient rate of medical management was 89.84%,the death rate was 10.16%, and the most common death causes were shock of severe bleeding, hepatic encephalopathy, and hepatorenal syndrome. Conclusion Sex,age, and season,etc. were related to upper gastrointestinal bleeding in patients with hepatic cirrhosis. Bleeding causes, liver function, complications, and age, etc. were factors affecting prognosis.