Acute leukemia is one of the most common cancers among children.The biological mechanisms leading to leukemia have not been fully clarified until now.Experiments were improved that most patients developing acute leukemia had abnormal chromosomes,including TEL/AML-l,BCR/ABL,PML/RAR-α fusion genes,which would be potential clinical biomarkers.Many genetic polymorphisms have effect on acute lymphoblastic leukemia susceptibility,which were containing genes involved in folate metabolism pathways,cytochrome P450,glutathione-S-transferase enzymes and quinone oxidoreductase-1.As the results of the studies,alcohol,tobacco,TNF-α and IFN-γ might be risk factors to the disease.Furthermore infection and IRF-3 were reported to reduce the occurrence of acute leukemia.Finally,as more is learned about the molecular pathology,it may be possible to develop new therapeutic agents which are specifically targeted to treat childhood acute leukemia.

The medical image registration between preoperative three-dimensional (3D) scan data and intraoperative two-dimensional (2D) image is a key technology in the surgical navigation. Most previous methods need to generate 2D digitally reconstructed radiographs (DRR) images from the 3D scan volume data, then use conventional image similarity function for comparison. This procedure includes a large amount of calculation and is difficult to archive real-time processing. In this paper, with using geometric feature and image density mixed characteristics, we proposed a new similarity measure function for fast 2D-3D registration of preoperative CT and intraoperative X-ray images. This algorithm is easy to implement, and the calculation process is very short, while the resulting registration accuracy can meet the clinical use. In addition, the entire calculation process is very suitable for highly parallel numerical calculation by using the algorithm based on CUDA hardware acceleration to satisfy the requirement of real-time application in surgery.
Algorithms ; Humans ; Imaging, Three-Dimensional ; Tomography, X-Ray Computed ; X-Rays

Superselective angiography and embolization is one of the important methods in the management of acute hemorrhage, providing mini-invasion and high efficacy, etc. The implication of angiography and embolization on bleeding were reviewed and the embolic agents as well as the interventional procedures were commented in this article.

Percutaneous radiofrequency ablation (RFA) has been widely used in the treatment of malignant hepatic tumors and has achieved satisfactory effects.Complications after RFA have been taken seriously and the risk of the development of complications is closely associated with tumor location.It is a great challenge for physicians to perform RFA for hepatic tumors in high-risk locations due to related difficulties and risks.This article reviews the complications after RFA for hepatic tumors in high-risk locations and analyzes related control strategies.

To explore the application of fetal DNA in maternal plasma for noninvasive prenatal diagnosis, the DNA template was extracted by hydroxybenzene-chloroform from 44 maternal (7-41 weeks) plasma. The Fetus-derived Y sequence DYZ-1 gene (149bp) was chosen to be amplified by PCR. The fragment was identified in all the plasma of male bearing pregnant women with the diagnostic accordance rate being 100.00%. Two of the 22 female bearing pregnant women had false positive results. Among the 44 pregnant women, the diagnostic accordance rate was 88.89% at early pregnant stage, 100.00% at medium pregnant stage, and 96.55% at late stage respectively. The final accuracy of 95.45% was obtained in all cases. It was concluded that by means of hydroxybenzene-chloroform extraction the authors of this article promoted the concentration and purity of the DNA template, and diagnosed more accurately. The results showed that free fetal DNA in the maternal plasma could be regarded as the gene resource for noninvasive prenatal diagnosis.
DNA/*blood ; DNA Primers ; Fetus ; Maternal-Fetal Exchange ; Pregnancy/*blood ; Prenatal Diagnosis/*methods ; *Sex Determination (Genetics)

Objective To study the regulative effect of angelica sinensis on cellular immune function in perioperative patients with obstructive jaundice. Methods Fourteen patients with obstructive jaundice were injected with angelica before and after operation for 14 days. The activity of IL-2 and the expression of IL-2R in lymphocytes in peripheral blood were measured, respectively. Results The activity of IL-2 and the expression of IL-2R decreased significantly in patients with obstructive jaundice (P

Objective:The purpose of this study was to assess the outcome of intestine fistula patients treated with home enteral nutrition(HEN) support before definitive operation.Methods:Fifty-eighty patients were divided into HEN support group and hospital enteral nutrition support group when their illness were stable and total enteral nutrition(TEN) were full resumed.All the patients were supervised by the members of nutrition support team.Days of TEN and hospital stay and medical cost was recorded.Plasma protein,body weight and body mass index were measured on the first day of full enteral nutriton and the day enteral nutrition was stop for operation preparation.Results:Nutritional status of the patients improved.There was no significant difference between two groups in nutritional status,complications and duration of TEN.The time patients in HEN group stayed at home was(61.11?27.71) d and in hospital(60.79?17.11) d.The cost in HEN group was less than that in hospital EN support group(140.21?61.2 vs 189.2?72.2 thousand Chinese dollar).Conclusion:Under the close supervise of nutrition support team,HEN is of the same safety and effect and can reduce the time of hospital-stay and medical cost when compared hospital EN.

Objective To study the effects of NEP1-40 on axon regeneration, motor function recovery of affected limbs and RhoA signal pathway in cerebral ischemia-reperfusion rats for exploring the possible mechanisms. Methods A total of 60 adult male Sprague-Dawley (SD) rats were equally divided into sham operation group (sham group), cerebral ischemia-reperfusion control group (control group), intra-lateral ventricle injection of PBS group (PBS group), and intra-lateral ventricle injection of NEP1-40 group (NEP1-40 group). The middle cerebral artery ischemia-reperfusion model (MCAI/R) was established by nylon monofilament occlusion method in rats. The changes in growth associated protein-43 (GAP-43) and RhoA expressions were determined by Western blotting technique. The motor function of affected limbs was tested by the "staircase test" of Montoya’s design. Results ① The expression of GAP-43 in NEP1-40 group was higher than that in the control and the PBS groups at 7 d and 14 d after MCAI/R, peaking at 7 d; ② The expression of RhoA in NEP1-40 group was significantly lower than that in the control and the PBS groups at each time point; ③ The results of "staircase test" in NEP1-40 group were much higher than those in the control group and the PBS groups at each time point after MCAI/R. Conclusion NEP1-40 can improve axon regeneration in cerebral ischemia-reperfusion rats and promote the recovery of motor function. The mechanism may be associated with the inhibition of RhoA signal pathway.

0.05). Conclusion: The IQ of children with epilepsy is lower than that of normal children .The epilepsy could impact the intelligence of children.

To study the role of the reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) gene and matrix metalloproteinase-2 (MMP-2) in the regulation of trophoblast invasion of early pregnancy. Immunohistochemistry, Western blot and gelatin zymography were used to detect the RECK protein expression localization, expression level and MMP-2 activation level in the placental tissues harvested from 52 normal pregnant women (27 in the early pregnancy, 25 in the term pregnancy). Immunohistochemistry showed that RECK expression was found both in villous tissues of early pregnancy group and term pregnancy group and was mainly observed in cell membrane and cytoplasm of cytotrophoblasts and syneytiotrophoblasts. RECK expression increased with gestational time. RECK expression of early pregnancy group was significantly lower than that of term pregnancy group (P<0.05). RECK expression was significantly lower in cellular column (CC) with invasion ability. Western blot showed that the RECK protein expression in early pregnancy group was significantly lower than that in term pregnancy (P<0.05). The optical density values of RECK protein expression in early pregnancy group and term pregnancy group were 1.35-0.14 and 2.68+/-0.26, respectively, while MMP-2 activation ratio was contrary to RECK protein expression and decreased with the gestation time (P<0.01). The MMP-2 activation ratios of early pregnancy group and term pregnancy group were 0.46 +/- 0.05 and 0.10+/-0.02, respectively. The expression of the tumor inhibitory gene RECK was positively related with the invasion ability of trophoblasts, while the invasion gene MMP-2 was negatively related with the ability. The interaction between RECK and MMP-2 may play an important role in the regulation of the trophoblast invasion in early pregnancy.