The event of “diagnosing mental disorder but actually not” violates patients′right of informed con-sent and refused to health , showing some problems of “diagnosing mental disorder but actually not” and mandatory admission process and psychiatric judicial authentication , and also have some ethical problems .Therefore, relevant departments should perfect the laws and regulation , establish a hospitalized psychiatric patients appeal , the appeal channels , at the same time , physicians should follow a correct understanding , respect patients rights , disinterest-ed, the ethical principles of self -supervision and also use constraints should be regulated .

OBJECTIVE:To evaluate the effect of temozolomide(TMZ)in combination with dexamethasone(DXM)on the proliferation of human gliocytoma U251 cells in vitro.METHODS:Human U251 cells were assigned to 1 of the 3 groups: TMZ(10,25,50,100,200,400?mol?L~(-1),respectively)alone or in combination with 40?mol?L~(-1)DXM(TMZ+DXM group)or control group(none drug).After treatment for 72 hours,the cell morphology,cell inhibition ratio,cell cycle and the apoptotic rate were detected.RESULTS:When TMZ concentration was greater than 100?mol?L~(-1)the cell inhibition ratio was higher in TMZ-treated group than in TMZ+DXM-treated group(P

BACKGROUND:Simple medication therapy can not promote the healing of articular cartilage defects effectively;the insufficient sources of autogenous cartilage also limited cartilage transDlantation.OBJECTIVE:The types of articular cartilage injury and local micro-environment changes were analyzed to summarize the progress of tissue engineered seed cell transplantation and cell transplantation therapy for repairing articular cartilage injury.METHODS:The databases of PubMed database(http://www.ncbi.nlm.nih.gov/sites/entrez/)and CNKI(http://www.cnki.net/)were retrieved with the key words of"Tissue engineering,cell transplantation,articular cartilage defects",and the literature was limited to English and Chinese languages.Studies concerning articular cartilage injury or was closely related to tissue engineered seed cells transplantatiOn were included.Repetitive studies were excluded Survival and migration of seed cells,as well as the recovery of joint function and adverse reactions were served as evaluation index.RESULTS AND CONCLUSION:A total of 201 literatures were seized by computers,according to the inclusion criteria,papers concerning tissue engineering cells for repairing articular cartilage injury were analyzed.Clinically,articular cartilage damage was commonly resulted by traumatic arthritis,which is difficult to repair due to the poor self-repair capacity of articular cartilage.The emergence of tissue engineered cell transplantation brought new hope for articular cartilage injury healing Cell transplantation has obtained good effects in articular cartilage repair,but the technology is still room for improvement.How to technically improve the tissue-engineered 3 elements,namely,cells,scaffold materials,and biological activity factors were the research focuses.Autologous cartilage regeneration was the theoretical support for articular cartilage defects repairing,which needs to optimize its regeneration and to maintain a more stable chondrocyte phenotype in further research The tissue-engineered cells had received good effects in repairing articular cartilage injury,and it is expected to be a new clinical treatment for articular cartilage injury with the in-depth study of tissue engineering.

BACKGROUND: Early rehabilitation following total knee replacement has arisen more attention. The aim of continuous passive joint motion is to recover knee function, prevent anchylosis, accelerate blood and synovia circulation, therefore, enhance tissue repair. OBJECTIVE: To evaluate the efficacy of continuous passive joint motion following total knee replacement. METHODS: Totally 48 patients, who underwent artificial total knee arthroplasty at the Department of Orthopedics, Center Hospital of Minhang District, Shanghai Ruikang Hospital Group and Department of Rehabilitation Medicine, 309~(th) Hospital of Chinese PLA between December 2007 and October 2009 were selected. The patients were randomly divided into the experimental and control groups, with 24 cases in each group. The isometric contraction of qudraceps muscles, combined with physical agent assistant, was performed prior to total knee replacement. Patients in the experimental group was received continuous passive joint motion training at day 2 after operation, with 5°-10°increasing per day. Patients in the control group were treated with conventional methods. The knee functional score, and visual analog scale (VAS) was performed to evaluate knee functions and pains, in addition, perimeter between two legs; and range of motion were measured. RESULTS AND CONCLUSION: The knee functional score, VAS, perimeter between two legs, and range of motion were similar prior to and after total knee replacement (P > 0.05). Compared to the control group, the knee functional score of the experimental group was dramatically increased at days 3 and weeks 2 after operation (P < 0.01), the VAS and perimeter between two legs were significantly declined (P < 0.01), the range of motion was obvious improved after replacement, with greater excellent rate (P < 0.01). There was no deep venous thrombosis or infections. The results demonstrated that early rehabilitation following knee replacement is conductive to easing pain, eliminating swelling, and enhancing knee stability.

Splenic radiofrequency ablation (RFA) is a safe, effective and minimally-invasive approach for the management of hypersplenism due to portal hypertension. After RFA, the remnant volume of the spleen will be decreased, the hypersplenism can be corrected, and the hepatic artery blood flow can be significantly increased with resultant marked improvement of liver function; in addition, hypertrophy and regeneration of the liver can be induced. However, many factors can affect the therapeutic results of RFA,therefore, further studies are necessary to clarify the underlying mechanisms.

Objective To establish a method of solid phase extraction-reversed-phase high performance liquid chromatography(SPE-HPLC)for determination of methomyl in plasma of rat.Methods The sample pretreatment method,the test conditions,the linear range,the sensitivity,the specificity,the precision, the accuracy,the stability and the recoveries for plasma were investigated by using rat plasma spiked with standard methomyl and intemal standard substance.Results The linear range was 0.1～20μg/mL ( r= 0.9993,P<0.001).The limit of detection was 0.03μg/mL(S/N ≥3).The intra and inter-day precision of assay for methomyl was less than 8.33%and 11.11%in plasma respectively.The intra and inter-day accuracy of assay for methomyl was between 90%and 120%in plasma respectively.The recoveries for methomyl were more than 88%±4.4%in plasma.Conclusion The HPLC method for quantitative and qualitative analysis of methomyl is simple,rapid and accurate,which is suitable for the identification of methomyl in the cases.

Radioimmunotherapy using monoclonal antibodies incorporated with radionuclide has been showed to be an effective agent for refractory non-Hodgkin's lymphoma (NHL). Many anti-CD20 antibodies labeled with radionuclide, such as 90Y-ibritumomab tiuxetan (Zevalin) and 131I-tositumomab tiuxetan (Bexxar), have been reported to be effective for the treatment of patients with relapsed or refractory low-grade, follicular, or transformed NHL. This review summarizes the current advance in clinical trials and studies of Zevalin and Bexxar for the treatment of NHL patients.

Objective To determine the frequency of the cholesteryl ester transfer protein (CETP)-TaqIB polymorphism and investigate its relationship with plasma lipid levels and coronary hert disease(CHD). Methods Two hundred and thirty-eight patients with CHD (CHD group) and 203 age-matched controls( control group) were selected, the CETp-TaqIB mutation was detected by restriction fragment length polymorphism of the CETP gene. Results In the total subjects, the frequency of B1 and B2 alleles were 59.4%(262/441 ) and 40.6%( 179/441 ) respectively. Compared with that in control group, the frequency of CETP genotype BIBI was higher in CHD group [39.9%(95/238) vs 29.6% ( 60/203 ), P<0.05], and the frequency of B1B2 was lower in CHD group [44.1%(105/238) vs 53.7%(109/203), P< 0.05]. Compared with that in the B2 homozygotes, high density lipoprotein cholesterol (HDL-C) and apolipoprotein (apo)A I level were significantly lower in the B1 homozygotes [(1.19±0.36) mmol/L vs (1.38±0.39) retool/L,( 1.17±0.33 ) g/L vs ( 1.30±0.31 ) g/L, P<0.05]. The B 1 homozygotes was associated with higher degree of cononary stenosis than the B2 carriers (P<0.05 ). There was no significant association between CETP-TaqIB genotype and the risk of CHD (P=0.147). Conclusions CETP-TaqIB polymorphism affects the concentrations of lipaproteins. There are significant associations between the B1 homozygotes and lowerHDL-C and apo A I levels. The B1 allele is not an independent risk factor for CHD.

BACKGROUND: As demonstrated by recent reports, immediate early genes (IEGs) are rapidly induced and expressed when a songbird is stimulated either by bird-song or by its vocal-behavior. The expression area and level of IEGs in the brain, such as zenk, c-fos and c-jun, are corresponding to where and how the neurons are related as a songbird is stimulated, suggesting that IEGs play important roles in vocal learning and memory.OBJECTIVE: To study the relation between vocal learning of songbird and immediate early gene so as to understand the mechanism of human language and the control of central nervous system.DATA SOURCES: All papers were cited from MEDLINE with the keywords of "songbird, vocal learning, memory, zenk, c-fos and c-jun" in English between January 1990 and December 2001. Meanwhile, we searched the Chinese journals full-text database and Wanfang database with the same keywords in Chinese between January 1990 and November 2001 were also searched.STUDY SELECTION: The papers about immediate early genes and vocal learning and memory in songbirds were selected. Then non-randomly controlled studies were excluded, and the rest full-text papers were looked up.DATA EXTRACTION: Totally 17 papers about immediate early genes and vocal learning and memory in songbirds were found. We choose 11 papers in them and 6 was excluded because of summarization.DATA SYNTHESIS: ① zenk and vocal behavior: Expression of zenk in bird's brain can affect learning and memory through adjusting synthesis of special protein. ② Reaction of c-fos to various songs: The reaction of local neurons in bird's brain to the birdsongs are made by the decision of the numbers of song factor in learning songs. ③ Associations of c-jun and cfos: zenk, fos and jun are the necessaries for maintaining long-term memory, which produce a marked effect through signal transduction, reconstruction, synthesis and transduction of induced synapse.CONCLUSION: Immediate early genes (IEGs) are rapidly induced and expressed when a songbird is stimulated either by bird-song or by its vocal-behavior. The expression area and level of IEGs in the brain, such as zenk, c-fos and c-jun, are corresponding to where and how the neurons are related as a songbird is stimulated, suggesting that IEGs play important roles in vocal learning and memory.