OBJECTIVE To investigate the spread status and drug-resistance of pathogenic bacteria causing chronic bacterial prostatitis among the senile patients,and provide the reference for clincal diagnosis and treatment.METHODS Prostatic fluid from the senile patients with chronic bacterial prostatitis was collected,and bacterial cultures and susceptibility tests were performed.The data were analyzed statistically.RESULTS Among 216 prostatic fluid specimens from the senile patients with chronic bacterial prostatitis,140 isolates of pathogenic bacteria were detected.In these strains,Gram-positive cocci accounted for 60.0% and most of them were Staphylococcus aureus.Gram-negative bacilli accounted for 40.0% and most of them were Escherichia coli.Pathogens causing prostatitis have been resistant to the common antibiotics.Among the total staphylococci,meticillin-resistant Staphylococcus(MRS) accounted for 34.8%.And among the total Enterobacteriaceae,ESBLs producing bacteria accounted for 35.6%.CONCLUSIONS Chronic bacterial prostatitis in the senile patients possesses intractability and persisting.It is important to monitor the pathogens and their drug-resistance.

OBJECTIVE To study the distribution and drug resistance supervision of infection pathogens of Gram-negative bacterial pneumonia in lower respiratory tract of senile patients and draft preventive and control measures.METHODS The sputum and lower respiration secretion of 368 senile patients were cultured to detect pathogens which were confirmed by routine methods.The disk diffusion test was adopted to analyze drug resistantce.RESULTS Pseudomonas aeruginosa rated the top one,reached 25.0%;the drug resistance supervision manifested that the resistant rates of Gram negative organisms to the routine drugs were high except for Haemophilus influenzae.And extensive-spectrum beta-lactamases(ESBLs) occurred with rate of 30.2%.CONCLUSIONS It is very necessary for clinical microbiology department to detect pathogens of lower respiratory tract pneumonia among senile patients and then to give reasonable advice for medicine in time.

AIM:To develop a method for determining cholic acid by HPLC-ELSD and GC was applied to determing muscone;in Jawei Xihuang Soft Capsule(Calculus Bovis,Moschus,Venenum Bufonis,Olibanum,Myrrha).METHODS:A C18 column(Kromasil C18,5 ?m,4.6 mm?250 mm)was used as stationary phase,the mobile phase was methanol-0.01% glacial acetic acid(73:27) at a flow rate of 1.0 mL/min.The parameters of ELSD were set as follows:evaporation temperature was 40 ℃,carrier gas(N2) pressure was 200 kPa.The GC system consisted of DB-1 capillary column(30 m?0.32 mm?0.25 ?m) and FID as the detector.The programmed temperature-GC and internal standard method were employed to determine the content of muscone.RESULTS:The linear ranges of cholic acid and muscone were in the range of 45.2 ng-904 ng and 0.05 mg/mL—0.5 mg/mL respectively.The average recoveries were 99.06% and 99.40% with RSD of 1.56% and 0.95% respectively.CONCLUSION:The method is convenient and accurate,and it can be used for the quality evaluation of Jawei Xihuang Soft Capsule.

AIM:To develop a method for determining cholic acid by HPLC-ELSD and GC was applied to determing muscone;in Jawei Xihuang Soft Capsule(Calculus Bovis,Moschus,Venenum Bufonis,Olibanum,Myrrha).METHODS:AC_(18) column(Kromasil C_(18),5 μm,4.6 mm×250 mm)was used as stationary phase,the mobile phase was methanol-0.01% glacial acetic acid(73:21)at a flow rate of 1.0 mL/min.The parameters of ELSD were set as follows:evaporation temperature was 40℃,carrier gas(N_2)pressure was 200 kPa.The GC system consisted of DB-1 capillary column(30 m×0.32 mm×0.25 μm)and FID as the detector.The programmed temperature-GC and internal standard method were employed to determine the content of muscone.RESULTS:The linear ranges of cholic acid and muscone were in the range of 45.2 ng-904 ng and 0.05 mg/mL-0.5 mg/mL respectively.The average recoveries were 99.06% and 99.40% with RSD of 1.56% and 0.95% respectively.CONCLUSION:The method is convenient and accurate,and it can be used for the quality evaluation of Jawei Xihuang Soft Capsule.

Objective To study metabolism of glucose and lipidin patients with hyperandrogenism and analyze the risk of their cardiovascular disease.Methods Object of study:firstly,choosed 118 cases of women of reproductive age from April 2013 to July 2014.Then detected their serum Testosterone (T),Estradiol (E2),fasting plasma Glucose (FPG),fasting plasma insu-lin (FINS),triglycerides (TG),total cholesterol (TC),high-density lipoprotein cholesterol (HDL-C),low-density lipopro-tein cholesterol (LDL-C),lipoprotein (a),body mass index (BMI).Finally,divided them into different groups and analyzed the results using SOSS19.0 software and two independent samples t-test statistical method.Results BMI,T,FPG,HOMA-IR and TG were significantly more higher (P <0.05)in the group of hyperandr-ogenism than in the group of normal con-trol.However,E2,TC,HDL-C,LDL-C and LP(a)had no significant difference (P >0.05)in statistics between the group of hyperandrogenism and the group of normal control.Hyperandr-ogenism group was divided into PCOS group and non-PCOS group according to etiology.In those two groups,BMI,T,E2,FPG,HOMA-IR,TG,TC,HDL-C,LDL-C and LP(a)had no significant difference in statistics (P >0.05).According to the definition of obesity adults in china,78 patients with hyperan-drogenism were divided into 2 group,one with BMI normal (BMI<24 kg/m2 )and the other group with overweight and obe-sity (BMI≥24 kg/m2 ).In group with overweight and obesity,BMI,T,FPG,HOMA-IR,TG,TC,LDL-C and LP (a)were significantly higher than the group with BMI normal (P <0.05),moreover,HDL-C was significantly lower than the group normal (P <0.05).E2 had no significant difference in statistics between the two groups (P >0.05).Conclusion Investiga-tion of the relation between hyp-erandrogenism and metabolism of glucose and lipid,can not only practically guide life style and drug therapy,but also prevent cardio-vascular disease.

Objective To study the clinical features ,the high risk features ,the treatments and the outcomes of chronic obstruc-tive pulmonary disease(COPD) patients combined with invasive pulmonary aspergillosis(IPA) .Methods The clinical dates of 48 COPD patients combined with IPA were retrospectively analyzed .Results The clinical manifestations of 48 COPD patients com-bined with IPA were dyspnea ,lung rales ,chest pain ,cough ,cough phlegm ,fever .Radiological findings were presented as pulmonary inflammatory exudation ,consolidation ,cavity of lungs .The treatments were effective in 16 cases ,ineffective in 32 cases .Conclusion Early diagnosis and treatment is important in COPD patients combined with IPA .

Objective: To investigate the effect and mechanism of liraglutide on vasodilatation of thoracic aortic ring in experimental rats.
Methods: The thoracic aortic rings were isolated from 32 male SD rats and divided into 2 groups: Without endothelium group and With intact endothelium group, n=16 in each group. The contractile force of vascular ring was detected and the effect of liraglutide (1×10-5 mol/L) on vasodilatation of norepinephrine (NE, 1×10-6 mol/L) pre-contracted ring was observed. The aortic ring in With intact endothelium group was further divided into 2 sub-groups:①the aortic ring was pre-treated by the inhibitor of nitric oxide synthase (NOS) (L-NAME at 10-4 mol/L),② the aortic ring was pre-treated by non-specific ATP-sensitive-potassium (KATP) channel (Glibenclamide at 10-5 mol/L), n=8 in each group. The impacts of liraglutide on those 2 sub-groups were studied.
Results: Liraglutide had no effect on isolated aortic ring at basic condition. Liraglutide at (10-5 mol/L) had vasodilatation effect in both Without endothelium and With intact endothelium groups, the effect was stronger in With intact endothelium group and the maximum vasodilatation reached 17%, P<0.05. In both pre-treated sub-groups, the effect of liraglutide on vasodilatation in sub-group①reached 4%, P<0.05, and the effect in sub-group②
reached 14%, P>0.05.
Conclusion: Liraglutide had obvious effect of vasodilatation on NE pre-contracted thoracic aortic ring in experimental rats, the mechanism might be related to NOS, while KATP channel could not block the vasodilatation effect of liraglutide.

Objective:To establish a method for the determination of nimesulide granules by HPLC. Methods:An Inertsil ODS-SP C18 column(250 mm × 4. 6 mm,5 μm) was used, and the mobile phase was 0. 01 mol·L-1 potassium hydrogen phosphate anhy-drous-acetonitrile(55∶45)[adjusting pH to (7. 2 ± 0. 05) with phosphoric acid]. The detection wavelength was set at 399nm and the flow rate was 1.0 ml·min-1. The injection volume was 20 μl. Results: The linear range of nimesulide was 0.044-0.222 μg(r=0. 999 9), the average recovery was 99. 22% and RSD was 0. 46%(n=6). Conclusion:The method is simple, accurate with promis-ing sensitivity and reproducibility, and can be used in the determination of nimesulide granules .

Objective To investigate the changes of serum cytokines IL-2,IL-8,IL-10 and IFN-? in infants with cytomegalovirus hepatitis before and after ganciclovir treatment,and explore the underlying mechanisms of ganciclovir and the immunological changes in the development and progression of cytomegalovirus(CMV) infection.Methods CMV-IgM in the serum samples of 72 infants with identified cytomegalovirus infection were measured by enzyme-linked immunosorbent assay(ELISA).CMV DNA in blood was measured by quantitative PCR.CMV-IgM(+) and CMV DNA(+) were as observed group 1(35 cases,without hepatitis).Those with abnormality of ALT were regarded as observed group 2(37 cases,with hepatitis).Another 30 matched healthy infants were enrolled as healthy control group.Levels of serum IL-2,IL-10,IL-8 and IFN-? of each group were examined by ELISA,and CMV masculine hepatitis group were cured with ganciclovir(5mg/kg,i.v.,twice per day for 14 d,and then once per day for 14 d after a 7 days'interval),then the levels of above factors were examined again.Results The levels of IL-8 and IL-10 in observed group 1 were significantly higher than those in healthy control group(t=3.74,9.56;P0.05).After ganciclovir treatment,the level of IL-2 in observed group 2 was significantly higher than that in observed group 1(t=3.41,P0.05).The levels of IL-8,IL-10 and IFN-? were decreased significantly(t=6.52,7.24,14.23;P

Aim To explore the mechanisms of Curcumin which plays a role of protecting cells through regulating the activity of GSK-3?.Methods Plasmid APPswe and BACE1-mychis were transiently co-transfected into SHSY5Y cells.The cells were treated with Curcumin and then were collected to determine the mRNA and protein levels of GSK-3?,?-catenin and CyclinD1 by RT-PCR and Western blot. Immuno-fluoresecnt staining was used to detect the location and expression of ?-catenin and GSK-3?, and ELISA was for the generation of A?.Results RT-PCR and Western blot results showed that in the cells, Curcumin inhibited the expression of GSK-3? mRNA and protein, and promoted the expression of GSK-3?-Ser9 protein (P