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Vitamin D,an immune regulator,plays an important role in innate immune function of antibacterial peptide.Vitamin D takes part in the innate immune function via toll-like receptor in response to pathogen,then release antibacterial peptide.This study describes the antibacterial peptide constitution and the functions,effection of activeted Vitamin D in innate immune function of antibacterial peptide.

As one of the chronic diseases,asthma,plays a serious impact on human daily life.Asthma in children has showed an increasing trend in recent years,but the mechanisms of asthma are not yet clear.Studies have found that store-operated calcium entry(SOCE) plays an important role in the physiological activity of the body.The enhanced SOCE activity can promote cell growth,proliferation,and migration of a variety of cell types.SOCE important molecules STIM1 and ORAI1 may be involved in the asthmatic airway occurrence of hyperresponsiveness and airway remodeling,and closely to the asthmatic development.

Minimal residual disease(MRD)iS the origin of relapse in leukemia.So it's significant to quantitate MRD in clinical.At present, there are three MRD quantitative assays, such as flow cytometric detection of abnormal immuno-phenotype,polymerase chain reaction(PCR)amplification of antigenreceptor genes and fusion transcripts.Because every measure has its own principle and feature.the clinical applications are different.Selecting suitable assays based on the characteristic of patients can help to quantitate MRD exactly.

?AIM: To observe the application of phacoemulsification combined with laser peripheral iridoplasty ( LPIP ) for acute angle-closure glaucoma with cataract which could not be controlled well by drugs.?METHODS:From January 2011 to June 2015, 49 eyes in 47 patients with acute angle - closure glaucoma and cataract were recruited while their intraocular pressure remained higher than 40mmHg 12h after drug treatment. LPIP were performed and phacoemulsification and intraocular lens implantation were carried out 3d after. Their clinical data and follow-up results were reviewed.?RESULTS: The intraocular pressure before treatment, 1d after LPIP, 1d after phacoemulsification, 1mo after phacoemulsification and 3mo after phacoemulsification were 62. 35+10. 31mmHg, 17. 96 ± 4. 64 mmHg, 16. 58 ± 3. 19mmHg, 13. 50 ± 2. 74 mmHg and 13. 46 ± 2. 48 mmHg respectively (F=10. 02,P<0. 05); the intraocular pressure of 1d (t=4. 35), 1mo (t=6. 43) and 3mo (t=6. 97) after phacoemulsification were all lower than the initial pressure (P<0. 05). The visual acuity showed the same trendy, while the visual acuity before treatment, 1d after LPIP, 1d, 1 and 3mo after phacoemulsification were 0. 06± 0. 02, 0. 20 ± 0. 18, 0. 45 ± 0. 19, 0. 60 ± 0. 11 , 0. 65 ± 0. 09 respectively (F=8. 36,P<0. 05). The best corrected visual acuity at 1d, 1 and 3mo after phacoemulsification were better than that before laser treatment ( t= 3. 97, 5. 12, 5. 89,P<0. 05). At 1d and 3mo after phacoemulsification, the anterior chamber depth, angle opening distance, trabecular/iris angle were all better than the initial ones (P<0. 05). At 1 and 3mo after phacoemulsification, the goniosynechia got better as well (P<0. 05).?CONCLUSION:Phacoemulsification combined with LPIP can reduce intraocular pressure, improve the visual acuity and make the anterior chamber depth, angle opening distance, trabecular/ iris angle, goniosynechia better for patients with acute angle-closure glaucoma and cataract when drug cannot control intraocular pressure well.

OBJECTIVE:To establish an HPLC method for determination of Emodin in Yanyan granules.METHODS:The stationary phase was Diamonsil C18(150mm? 4.6mm,5? m)column and the mobile phase was methanol-0.1% phosphoric acid(68∶ 32),with detection wavelength set at 254nm.RESULTS:Within the range of 0.26～ 3.90? g Emodin had a good linearity,and the average recovery was 96.73%(RSD=1.49%).CONCLUSION:The method is accurate,sensitive,reproducible,simple and can be used for the quality control of Yanyan granules.

[Objective] To observe the effect of electroacupuncture (EA) on learning and cerebral heme oxygenase (HO) activity in rats with vascular dementia (VD). [Methods] Sixty rats were randomly allocated to mimic group (Group A), EA group (Group B), nimotone group (Group C) and model group (Group D) . Rat models of VD were established by 4 - vessel occulusion (4 - VO) method. Ten days after modeling, Group B was trested with EA on Baihui (GV20) and Dazhui (GV14), one time per day; Group C with nimotone, 20ml/kg body weight, ig, qd. The treatment course lasted 15 days. And then the learning was estimated with Morris water maze and HO activity in cortex and hippocampus (HC) of rats was also measured. [Results] Mean escape latency within 6 days in EA group was shorter than that in model group ( P 0.05). In EA group, the swimming times in the original platform quadrant were more than that in the right, left or opposite quadrant and also more than that in the original platform quadrant in the model group (P

Objective:To research the effect of total saponins Panax ginseng (TSPG) on the drug resistance of human erythroleukemia drug resistant cell line K562/A02 and its mechanism.Methods:The sensitivity of TSPG-treated K562/A02 cells to anticancer drugs was determined by MTT assay.Immunohistochemistry method was used to measure mdr-1 gene product P-gp and apoptosis regulation gene product bcl-2 expression. RT-PCR technique was used to examine mdr-1 mRNA expression.Results:In non-toxic dosages, TSPG strengthened the toxicity effect of doxorubicin on K562/A02 cells, while the reversing effect of TSPG was related to its concentration. Decrease in P-gp expression in K562/A02 cells was marked ( P O. 05)after 3 days of TSPG(150?g/ml)treatment.Decrease in mdr-1 mRNA expression was significant ( P

Objective:To investigate the neuroprotective role of hemin and its effect on the expression of neuroglobin in the hippocampal organotypic tissue cultures(HOTC)after combined oxygen/glucose deprivation(OGD).Methods:SD rats,8~10-days old,were sacri-ficed for brain.HOTC were made and randomly divided into three groups:HOTC,HOTC undergoing OGD(OGD),HOTC under- going OGD following pretreatment with hemin(Hemin+OGD). The expression of neuroglobin was detected by immunohisto-chemical technique and computer-assisted image analysis.Results:In OGD group,hippocampal organotypic tissue lost normal structure and neurons were damaged severely.Pretreatment with hemin could effectively reverse the damage of the neurons.The expression of neu- roglobin in the Hemin+OGD group were significantly stronger than that in the OGD group.Conclusion:Hemin has protective effect on injury induced by OGD in rat hippaocampal slices,which is associated with enhanced expression of neuroglobin.

Objective:To discuss the different expression of AQP8 in cultured normal human trophocyte and JAR cell line,and the relationship between the changed expression of the channel protein and nosogenesis of chorionic carcinoma.Methods:The human cultured normal trophocyte was primarily cultured,purified and identified.JAR cell line was cultured.The expression of AQP8 was detected in cultured normal human trophocyte and JAR cell line by immunohistochemistry immunofluorescence confocal laser and RT-PCR.Results:(1)In the control group,the limitied expression of AQP8 was observed in cultured normal human trophocyte and JAR cell line,but in the exprimental group,the rate of positive cell of AQP8 in JAR cell line were 93.27%?14.45%,much higher than that in the control group.(2)By immunofluorescence staining confocal laser imaging,it was revealed that,in the same condition and excitation intensity,AQP8 in the JAR choriocarcinoma cell lines showed positive expression of the green,while in normal trophoblast cells,no green light,but when the excitation intensity was strengthened,it may still explore AQP8 in normal human villous trophoblast cell expression(.3)The expression of AQP8mRNA was detected in both cultured normal human trophocyte and JAR cell line.The OD value(0.98?0.35)of AQP8 in the experimental group was significantly higher than that(0.32?0.04)in the control group.Conclusion:Abnormal overexpression of AQP8 will destroy the external water and electrolyte balance of trophoblast cells,which might be one of the reasons for the occurrence of choriocarcinoma.