Objective To observe the intrapartum analgesic effect of Doula GT-4a pulse acting working station and the influence of it on labor and mother-to-child. Methods The observation group use intrapartum analgesia of 98 primigravida,and the control group was other 98 primigravida without intrapartum analgesia in the same delivery period. Two groups of gravidas were delivering by a high qualification of midwife while your cervix expand for 2 ～3cm. The observation group was given with GT-4a pulse acting working station. Results The analgesia VAS scores of the observation group,significantly different with that of the control group:The first stage of labor active period accelerated significantly,the second stage of labor was shortened. The cesarean section rate,the vaginal birth rate and the neonatal mild choking rate significantly declined compared with the control group. Though there were no statistical differences between two groups with the third stage of labor, postpartum hemorrhage, and neonatal severe choking rate.Conclusion The andlgesie effect of doula combined with GT-4 pulse theraphy for labor was good. It reduced cesarean section rate, improved maternity quality and had no adverse effects.

The fatty acid components and microviscosity of erythrocyte membrane in 39 type 2 diabetic patients with and without coronary heart disease(CHD) were examined by high-performance liquid chromatography (HPLC) and fluorescence polarization teachnique,and their relationship with insulin sensitivity index (ISI) and CHD were analyzed.The results showed that contents and composition of erythrocyte membrane arachidonic acid (AA,C20:4)were significantly lower in type 2 diabetic patients with or without CHD than those in control subjects (P＜0.01 and P＜0.05).The total fatty acids contents were also significantly lower in patients with CHD than those in normal subjects (P＜0.05).In patients with or without CHD,erythrocyte membrane microviscosity was significantly increased as compared with the controls (P＜0.01 and P＜0.05),and it was higher in diabetics with CHD than those without CHD (P＜0.05).AA contents of erythrocyte membrane was negatively correlated with microviscosity and positively correlated with insulin sensitivity index (ISI),while the microviscosities were negatively correlated with ISI in diabetics.AA,linoleic acid contents,microviscosity of erythrocyte membrane and ISI were all correlated with the incidence of CHD in diabetics.In diabetic patients,the fatty acid metabolic abnormality could cause the alterations of functions,structures and fluidity of erythrocyte membrane,thus may contributing to increased IRS and might be related to the incidence of CHD.

Objective To investigate the changes of serum cytokines IL-2,IL-8,IL-10 and IFN-? in infants with cytomegalovirus hepatitis before and after ganciclovir treatment,and explore the underlying mechanisms of ganciclovir and the immunological changes in the development and progression of cytomegalovirus(CMV) infection.Methods CMV-IgM in the serum samples of 72 infants with identified cytomegalovirus infection were measured by enzyme-linked immunosorbent assay(ELISA).CMV DNA in blood was measured by quantitative PCR.CMV-IgM(+) and CMV DNA(+) were as observed group 1(35 cases,without hepatitis).Those with abnormality of ALT were regarded as observed group 2(37 cases,with hepatitis).Another 30 matched healthy infants were enrolled as healthy control group.Levels of serum IL-2,IL-10,IL-8 and IFN-? of each group were examined by ELISA,and CMV masculine hepatitis group were cured with ganciclovir(5mg/kg,i.v.,twice per day for 14 d,and then once per day for 14 d after a 7 days'interval),then the levels of above factors were examined again.Results The levels of IL-8 and IL-10 in observed group 1 were significantly higher than those in healthy control group(t=3.74,9.56;P0.05).After ganciclovir treatment,the level of IL-2 in observed group 2 was significantly higher than that in observed group 1(t=3.41,P0.05).The levels of IL-8,IL-10 and IFN-? were decreased significantly(t=6.52,7.24,14.23;P

Objective To observe the clinical effect of Herba Houttuyniae Injection in treating senile pneumonia and to explore its possible mechanism. Methods Sixty-five patients were randomly divided into two groups. The control group was treated with conventional western therapy (CWT), while the treatment group was treated with CWT plus Herba Houttuyniae Injection. The changes of serum cytokines, IL-2 and TNF-?, before and after treatment were observed. Results the total effective rate of treatment group is 94.3%, while that of the control group is 83.3%. The difference of the total effective rate between the two groups was significant(P

The article reported that the lipid and EPA were extracted from Chlorella hirataii and analyzed by GC method. The content of the lipid could be influenced by the time and conditions of the culture. The results showed that the content of EPA in the lipid of Chlorella hirataii was about 28 per cent and was higher than the content of EPA in fish oil. Therefore, Chlorella hirataii was a valuable resource rich in EPA.

Objective Through retrospective data analysis,we tried to further understand the epide-miological characteristics,clinical feature and death factors of infant and young children with severe pneumo-nia. Methods The study objects were inpatients( age between≥28 days and≤3 years) who were diagnosed severe pneumonia from 1 January,2011 to 31 December,2013 of the Chengdu Women′s and Children′s Cen-tral Hospital. We used retrospective case study to understand the epidemiology,clinical feature,death factors of infant and young children with severe pneumonia. And we used chi-square test and Logistic multivariate regression analysis to analyze the death factors of infants and young children with severe pneumonia. Results (1) Among 1 411 cases of severe pneumonia,the ratio of male and female was 1. 8∶1,and the ratio of urban and rural areas was 1∶3. 62. The proportion of less than 3 months old infant was 46. 00%. And 62. 93% infant and young children with severe pneumonia occurred in the spring and winter. (2) Average hospitalization time was (9. 99 ± 6. 27 ) days, longer than the hospitalization time of mild pneumonia patients. ( 3 ) A total of 64. 21% of infant and young children with severe pneumonia had basic diseases. (4)A total of 91. 99% of the infant and young children with severe pneumonia had complications. (5) The most common etiology of infant and young children with severe pneumonia was bacteria,the second was virus. (6) In all cases,there were 44 cases died. The mortality of infant and young children with severe pneumonia was 3. 12%. And 72. 73% of the death cases were infants less than 3 months old. (7) The results of Logistic multiple regression analysis showed that there were significant differences in age, congenital heart diseases, repeating infection history, multiple drug-resistant strains infection, surgical history, multiple organ dysfunction, internal environment disorder. Conclusion Infant and young children with severe pneumonia have the following characteristics:most of them occurred in the winter and spring, and come from rural more than from the city. The smaller the age, the incidence of a disease is higher,and the mortality is higher. Most of infant and young children with severe pneu-monia have basic diseases. Most of the infant and young children with severe pneumonia have complications. If having one of the following high-risk factors:less than 3 months old,congenital heart diseases,repeating infec-tion history,multiple drug-resistant strains infection,surgical history,multiple organ dysfunction,internal envi-ronment disorder,the infant with severe pneumonia should be intensively monitored and actively treated.

Objective: To explore the infection of mycoplasma in the venereal diseases and information about their drug susceptibility. 　Methods: Materials from the 12 528 patients were cultivated and drug susceptibility made testing was made using the mycoplasma IST Kit.　Results: The results showed that 9433 of 12 528 (75.3%)were infected by mycoplasma, Uu infective rate 58.3 %(7 305 cases)was found significantly higher than Mh infective rate 3.0%(379 cases)and mixed infection of both Uu and Mh was 14.0%(1 749 cases,)P＜0.01. The no susceptible conditions were in turn that ofloxacine(50.5%), Erythromycine(41.9%), tetracycline(14.6%), doxycycline(4.7%), josamycine(2.9%), and pristinamycine(2.0%) respectively.　Conclusions:The results suggested that non-gonococcal genitourinary tract infection was firstly ureaplasma urealyticum, concentracted infection was at age 21-40 years. no susceptible stat of mycoplasma infection to drug was serious, so that susceptibility testing of mycoplasma had a important significance to clinical drug treatment.

Background Paired box gene 6 (Pax6) is a master regulator for eye and brain development.Pax6 mutations or changes in its expression cause a series of ocular diseases including absence of iris,corneal opacity,cataract,glaucoma,abnormal fovea,retinoblastoma,and Wilm's tumor-aniridia-qenital ahormalies-retardation (WAGR).As a transcription factor,it is expressed in the region of anterior surface ectoderm corresponding to the future adenohypophyseal,olfactory and lens placodes,optic vesicle and other parts of the future brain and thus control the development of eye,brain,pituitary grand,nose and pancreas.Pax6 exists in 4 different isoforms,whose functions are subjected to regulation by different post-translation modifications.A complete understanding of the structure and functions of Pax6 and its associations with relevant diseases is helpful for ophthalmologists to investigate the pathogenesis and treatment of implicated ocular diseases caused by Pax6 gene mutation or changing in its expression.

In this paper,we summarized nursing care of 9 patients with cytokine release syndrome after receiving the chimeric antigen receptor T cells immunotherapy.Key points of nursing care were as follows:comfortable and effective antipyresis,preventive rehydration,multiple methods of blood oxygen saturation improvement,iatrogenic pressure ulcer prevention,frequent vital signs monitor,close observation of level of cytokine and inflammatory factors,timely and accurate medication,formulating personalized nursing plan according to patients' condition,close collaboration between doctors and nurses.At last,8 patients recovered and were discharged,and 1 case died due to multiple organ dysfunction syndrome.

The preventive mechanism of salviae miltiorrhizae (SM) against experimental atherosclerosis (AS) in rabbits models was investigated. The experimental AS rabbit models were reproduced by feeding the high cholesterol diet. The changes of atherosclerotic plaques in normal group, model group and SM treated group were observed. The levels of serum TG, TC, HDL-C and LDL-C were determined. The immunohistochemistry was used to detect the expression of Bcl-2, Bax and IL-6 proteins in atherosclerotic plaques. The results showed that the level of serum TG in SM treated group was significantly lower than in model group (P<0.01). Immunohistochemistry revealed that the expression of Bcl-2, Bax and TL-6 in model group was significantly higher than in normal group. In the SM group, the expression of Bcl-2 protein was up-regulated and that of Bax was down-regulated. It was suggested that SM could inhibit formation of AS in experimental rabbits. To decrease the expression of Bax and increase the expression of Bcl-2 protein may be one of the mechanisms of SM against atherosclerosis.
Atherosclerosis/etiology ; Atherosclerosis/*prevention & control ; Diet, Atherogenic ; Drugs, Chinese Herbal/*therapeutic use ; *Phytotherapy ; Proto-Oncogene Proteins c-bcl-2/biosynthesis ; Random Allocation ; *Salvia miltiorrhiza ; Triglycerides/blood ; bcl-2-Associated X Protein/biosynthesis