In the teaching reform of cytobiology,we should first formulate a reasonable syllabus according to training goals,pay attention to course content reform,strengthen connection with other preclinical medicine subjects,combine quality-oriented education in class instruction,and cultivate students scientific and original spirit.The experimental courses should be offered from higher level and be conformed with subject development.The teacher should have innovative thinking,adopt diversified teaching methods,and fully arouse students' study enthusiasm.

Objective To probe into the application feature and use tendency of traditional Chinese medicine (TCM) injections in our hospital;To evaluate rational drug use and provide reference for related researches. Methods TCM injections in our hospital during 2010-2012 were analyzed by employing the analytic method of defined daily dose, with a purpose to evaluate frequency of drug use and rationality of medication expenses. Results From 2010 to 2012, drug possession rate of TCM injections in our hospital was 4.07%-4.47%;the amount of medication accounted for 10.50%to 12.69%of the amount of all drugs;the annual growth rate increased significantly, among which TCM injections used for removing blood stasis took the lead;frequency of drug use rose annually;medication synchrony in 3 years indicated that daily expensesof Shenkang Injection, Aidi Injection, salvianolateinjection, safflor yellow sodium chloride injection and other TCM injections were on the high side. Conclusion From 2010 to 2012, TCM injections in the clinical application in our hospital were of relatively high frequency, and the TCM injections used for removing blood stasis took the lead. Daily expenses of TCM injections drugs were on the high side and there may be unreasonable application.

Objective To investigate the relationship between the serum concentration of amiodarone and QT dispersion.Methods The serum concentration of Am and DEA were determined by HPLC during the treatment in forty Patients to study the relationship between the serum concentration and QTd.Results After using amiodarone,QT interval was significantly increased[(383±31)ms vs(418±29)ms,P＜0.05].In the same time QTd was significantly decreased[(34±12)ms vs(21±9)ms,P＜0.05].With maintaining dosage,the serum concentration of DEA had positive relationship with the increment of QT(r=0.50,P＜0.05).While with loading dosage,the serum concentration of amiodarone had no relationship with it.The serum concentration of amiodarone had no relationshiP with the decrement of QTd in all patients.And it varied in a large range.Between the groups of different therapeutic effect,the serum concentration had no statistical difference.Two patients appeared the adverse effects.Their serum concentration of amiodarone weren't higher than the average concentration of the group.Conclusion The serum concentration of amiodarone couldn't be an index tO estimate the effect and the occurrence of adverse effects.

Tripterygium Hypoglaucum Hutch and Tripterygium Wilfordii Hook.f are all Evonymus alata family Tripterygium Wilfordii genus plants.They are generally used in the clinical treatment in department of dermatology.They resemble each other not only in some chemical constitutions,but also in some pharmacologic actions(such as anti-inflammatory,anti-tumor,immune suppression,antifertility and so on),but they are all poisonous plants.When they are used for therapy,they can induce some toxic reactions,even the death of patients.In order to facilitate the adoption of the two drugs,we review their pharmacologic actions.

A new method of the separation of hydrocarbons and glycerides in liver oil of Mustelus jriseas was designed. The Waters Prep-500A Liquid Chromatograph with silica gel column and differential refractive index detector was used. The mobile phase was petroleum ether and ethyl acetate. The pure hydrocarbons' and glycerides were obtained from the liver oil.

BACKGROUND:The establishment of animal pulmonary hypertension models will promote the improvement of clinical diagnosis and treatment on pulmonary hypertension.OBJECTIVE:To analyze and summarize the establishment of animal pulmonary hypertension models.METHODS:The databases of PubMed and CNKI were retrieved with key words of "pulmonary hypertension and animal model".Studies concerning the following aspects were included:using surgery method to prepare animal pulmonary hypertension models;using drug injection method to prepare animal pulmonary hypertension models;animal severe pulmonary hypertension model;pathophysiological comparison between animal models and pulmonary hypertension patients;and drug intervention on animal pulmonary models.RESULTS AND CONCLUSION:The methods for animal pulmonary hypertension models construction including:Surgical shunt,monoerotatine injection,chronically hypoxic and so on,in addition,the animal several severe pulmonary hypertension models which appeared at distal pulmonary artery neointimal lesion formation was reported.Through continuous improvement,they have provided insights into pathological cellular and molecular signaling pathways.However,how closely any of the models simulate to the cellular and molecular pathobiology of human pulmonary hypertension was poody understood.

Chemotherapy plays an important role in advanced colorectal cancer. Patients may show various clinical response to selection different drugs. The importance of individual therapy based on pharmacogenomics has been emphasized in cancer therapy. To explore the individual therapy of colorectal cancer, the progress of molecular markers which influence the efficacy of anti-tumor drugs containing fluorouracil, oxaliplatin, CPT-11, monoclonal antibody drugs is reviewed in this article.

Objective To probe into the chnical value of strain rate imaging on the assessment of left atrial function in patients with hypertensive disorder comphcating pregnancy(HDCP).Methods Thirty two patients with HDCP and 20 control subjects were involved.Patients with HDCP were divided into 2 groups:17 with normal left ventricular mass index (LVMI) and 15 with left ventricular hypertrophy.These patients and controls underwent transthoracic echocardiography,strain and strain rate imaging examination.Atrial strain and strain rate imaging values of patients with HDCP were compared with those of controls.Multiple parameters were compared by one-way anova analysis.Results When compared with the controls,peak early diastolic strain rate (ESR) was significantly decreased in HDCP patients with normal LVMI and left ventricular hypertophy [(-1.13 ± 0.15),(-1.04 ± 0.29) s-1 vs.(-1.26 ± 0.23) s-1,P ＜ 0.05].Compared with the normal LVMI,peak late diastolic strain rate (ASR) was significantly decreased in controls and patients with left ventricular hypertrophy [(-1.23 ± 0.25) s-1 vs.(-1.36 ± 0.33),(-1.34 ± 0.32) s-1].There was no significant difference in the others (P ＞ 0.05).Conclusion Left atrial function is decreased in HDCP patients,which with left ventricular hypertrophy,the tendency is more obvious.

In 161 cases of the lung cancer, serum values of CEA. CIC. ACE. CRP. IgE and ferritin were determined. It was found that their serum CEA. CIC. CRP and ferritin were higher, whereas serum ACE was lower than that of normal healthy subjeots and those suffering from benign pulmonary diseases. Serum CEA and CIC were distinctly lowered following surgical resection, radioor chemotherapy while the serum ACE activity was slightly elevated. Simultaneous determination of ferritin and CEA might increase the chance for detection of the lung cancer. The results of the study showed that determination of multiple indices are helpfui in the diagnosis, evaluation of the therapeutic response detection of relapse and prognosis of the lung cancer.

Thrombin-induced aggregation between mononuclearcytes and platelets was investigated and shown to be calcium dependent. Tetrame-thylpyrazine inhibited the aggregation and a dose-effect relationship was demonstrated. Tetramethylpyrazine also prevented the decrease of blood leukocyte count induced with thrombin venous injection in mice. Angelica sinensis Diels and tanshinone did not show any obvious effects on thrombin-induced aggregation between mononuclearcytes and platelets.