The effects of nitrendipine (Nit) on myocardial contractility, myocardial succinate de-hydrogenase (SDH) and cytochrome oxidase (CCO) activities in streptozotocin (65mg/kg) diabetic rats were studied. Four weeks after the induction of diabetes, the rats were treated with Nit (30mg ?kg -2/day) for 4 weeks. The results showed that ventricular diastolic function was affected after 4 weeks of diabetes, and both ventricular diastolic and systolic functions were obviously involved after 8 weeks. These changes were significantly improved in diabetic rats receiving Nit treatment. The myocardial SDH and CCO activities in diabetic animals were markedly lower as compared to controls. The attenuation of these enzyme activities in diabetic rats was significantly reversed by administration of Nit. These findings suggest that Nit treatment may exhibit some beneficial effects on diabetic cardiomyopathy.

Objective:To investigate the relationship between poly(ADP-ribose)polymerase(PARP) inhibition and the growth activity of murine colon carcinoma CT26 cell lines in vitro.Methods:The murine colon carcinoma CT26 cell lines were treated with PARP inhibitior 5-AIQ in vitro.MTT assay was used to determine the growth activity of CT26 cells.The expressions of PARP and NF-?B p65 in the nuclei of CT26 cells were investigated by double labelling immunofluorescence and laser scanning cofocal microscope.Results:The inhibitory rates of the growth in 5-AIQ-treated colon carcinoma CT26 cell groups were 17.52%、27.63% and 39.93%,respectively.The inhibitory rate increased with the raising of the 5-AIQ concentration(0.1mmol/L,0.5mmol/L,and1.0mmol/L),and the difference was significant(P

The pathogenesis of barium-induced severe myocardial necrosis in dietary potassum-depleted rats was investigated. In this study four experiments were performed. The results of these experiments suggest that there exist a complex pathogenesis of myocardial lesion produced by barium in condition of potassium deficiency. The main mechanism seems to be the direct stimulating effect of barium on myocardial contractibility. It seems unlikely that barium-induced constriction of coronary arteries, hypertension, hypokalemia, and increased catecholamine secretion are the basic mechanism of the myocardial necrosis. But these factors may play a promoting or aggravating role in the development of the myocardial lesion. Potassium deficiency not only acts as a conditioning factor for barium-induced myocardial necrosis, but also relatively increases the cardiotoxity of barium. This may be the principal pathogenesis of severe myocardial necrosis produced by barium in combination with potassium deficiency.

ObjectiveTo investigate the effect of down-regulating epidermal growth factor receptor (EGFR) expression on Bleomycin induced pulmonary fibrosis in mice.MethodsForty 4 ～6 week aged C57BL/c male mice were randomly divided into control,bleomycin,bleomycin plus EGFR RNAi groups and RNAi negative control group.Bleomycin group were treated with bleomycin (3 mg/kg) by endotracheal injection on day 0,control group were treated with PBS.And bleomycin plus EGFR RNAi group were received EGRF siRNA plus bleomycin intratracheal administration.RNAi negative control group received negative EGRF siRNA plus bleomycin intratracheal administration.Mice were sacrificed 10 days after the treatments.Hydroxyproline (HYP) assay was performed in the lung tissue.The lung tissue slides were examined pathologically with H.E.staining,and EGFR mRNA expression was detected by reverse transcriptasepolymerase chain reaction (RT-PCR) technique.Western blot were performed to identify the protein level.of total EGFR and phosphorylated EGFR.ResultsHistological examination of lung specimens demonstrated that EGFR siRNA administration lessened lung fibrosis induced by bleomycin and significantly reduced HYP content (543.00±25.89 vs 900.73±31.77,P＜0.01).EGFR mRNA (0.31±0.05 vs 0.75±0.08,P ＜0.01) and protein expression(1.53±0.47 vs 2.56±0.37,P ＜0.01) in EGFR siRNA-treated mice was significantly decreased.The expression of phosphorylation of EGFR protein (1.78±0.35 vs 2.84±0.51,P ＜0.01) and EGFR protein in RNAi group was less than in bleomycin group.Conclusions EGFR RNAi reduced the BLM-induced lung fibrosis by inhabiting EGFR activation.

ObjectiveTo study the clinical significance of serum type Ⅳ collagen( Ⅳ - C) levelsfor the diagnosis of hepatic fibrosis. MethodsSerum levels of Ⅳ - C were measured in 209 patientswith viral hepatitis and 53 healthy persons by ELISA. ResultsCompared with the normal group,the serum Ⅳ - C level was significantly increased in patients with chronic moderate hepatitis and wasthe highest in patients with chronic severe hepatitis and hepatic cirrhosis( P < 0.01 ). There was no sig-nificant difference in the serum Ⅳ- C level among acute viral hepatitis, chronic mild hepatitis and con-trols ( P >0.05). There was significant difference in the serum Ⅳ - C level in patients with hepaticcirrhosis by Child- Pugh grading( P < 0.01 ). The serum Ⅳ - C level was positively correlated withthe degree of hepatic fibrosis and the severity of hepatic diseases. ConclusionThe meastrement of serumⅣ- C may be useful for assessment of the degree of hepatic fibrosis and progrosis of chronic hepatitis.

Hydroxyapatite artificial eye mount has no toxicity,no sensitization and good histocompatibility.Its porous structure is beneficial for the ingrowth of new vessels and fibrous tissues,thereby it is the main implant of orbital reconstruction following enucleation.With the wide application in clinics,hydroxyapatite artificial eye mount also show some complications such as conjunctival humbling,erosion,split and artificial eye mount exposure.There are many causes for these complications and the most one is the stimulation and wearing of orbital soft tissues induced by crude implants,as well as the delaying in vessels.Using autologous or variant tissue for covering or encapsulating the anterior artificial eye mount,the mount will be strengthen and become smooth,thereby reducing the friction.Autologous sclera is superior to other encapsulating materials.

To discuss the ultrasonic testing intelligent infusion pump tubing bubble method. When the ultrasonic wave goes through the interface of two different media, the reflection occurs, refraction, transmission and other physical phenomena can occur because of a large extent acoustic impedance difference between water and air, which can be used in this physical phenomenon, by monitoring the ultrasonic energy (output voltage) can be obtained if the extent of the presence of bubbles and bubble exists within the infusion tube. By measuring the penetration of ultrasonic energy, we detected air bubbles within the infusion line. Ultrasound can accurately detect air bubbles in the infusion line, which is the main method of detecting air bubbles for infusion pump.

In the current multimedia application of medical theory teaching there exist many problems such as infusion,relation estrangedness between teachers and students,the moral education marginalization,the abstract thinking ability attenuation and so on.Facing these problems,in the medical theory teaching,the multimedia application should take"help","reality","transfer"as the value pursue,and be located in the exaggeration atmosphere,the interest stimulation,induction inspiration,difficulty breakthrough,thought expansion and memory optimization,emotion cultivation and moral promotion.

BACKGROUND: In association with advanced startover of hypothalamus-pituitary-sexual gland axis, the growth of skeleton is accelerated and epiphyseal fusion occurred earlier in puberty precocity children. In clinic,Chinese drug for nourishing yin and reducing fire retards bone age and the advanced fusion in the disease. At the age of sexual development, Chinese drug for benefiting kidney to supplement essence is replaced to promote sexual development and accelerate skeleton growth. The patients with puberty precocity are the same to that in normal puberty in sexual development and bone growing, just being advanced in time. But, it is still unknown the mechanism of Chinese drugs on bone growth and effects on somatostatin (SS) in bone growth.OBJECTIVE: To explore the regulation of kidney-nournishing Chinese drug on periventricular nucleus (PeVN) SS.DESIGN: Completely randomized group division and control experiment were designed.SETTING: Department of Endocrinology, Children's Hospital Affiliated to Shanghai Jiaotong University MATERIALS: The experiment was performed in Institute of Pediatrics of Children's Hospital Affiliated to Shanghai Fudan University from October 2001 to April 2002, in which, 24 SD white rats were employed, of purebred, female and clean grade. 4 groups were randomized, named the control A, the group with drugs of nourishing yin and reducing fire (experiment group No. 1), the control B and group with drugs of nourishing yin and reducing fire + benefiting kidney to supplement essence (experiment group No.2),6 rats in each group.METHODS: ① Control A:physiological saline was applied for gastricperfusion, 5 mL each time, once per day, totally for 1 month. Experiment No. 1 group: Chinese mixture for nourishing yin and reducing fire was applied for gastric perfusion, composed of shengdihuang (Radix Rehmanniae),zhiguiban (Chinemys reevesii.), huangbai (Cortex Phellodendri) and zhimu (Rhizoma Anemarrhenae) etc, 5 mL each time, once per day, totally for 1 month. Control B: physiological saline wasapplied for gastric perfusion,5 mL each time, once per day, totally for 2 months. Experiment No.2 group:Firstly, Chinese mixture for nourishing yin and reducing fire was applied for gastric perfusion, 5 mL each time, once per day, totally for 1 month.And then Chinese mixture for benefiting kidney to supplement essence was applied for breeding, composed of shudihuang (Radix Rehmanniae Preparata), guibanjiao (Colla Carapacis Testudinis), xianlingpi, lujiaojiao (Colla Cornus Cervi) etc., 5 mL each time, once per day, totally for 1 month.② In situ hybridization staining procedure with floating and routine immunohistochemical technique. Medical imaging analysis software was used to assay PeVN SS mRNA, SS immunoreactive neuron counts and absorption (A value) of positive cell area that reflects the gene expression and protein expression. Number of positive neurons of unit area refers to the positive neuron counts in each square millimeter.③ Singer-factoranalysis of variance was used for the comparison of measurement data. F test was adopted for difference comparison among groups.MAIN OUTCOME MEASURES: Effects of Chinese drugs for nourishing yin and reducing fire and benefiting kidney to supplement essence on PeVN SS gene mRNA and protein expressionRESULTS: 24 rats were all in result analysis. ① PeVN SS mRNA positive neuron counts, positive neuron counts of unit area and A value of positive cell area: Those in experiment No.1 group was higher remarkably than the those of control A (P ＜ 0.05); and those in experiment No.2 group were lower remarkably than those of experiment No. 1 group (P ＜ 0.05). ② PeVN SS positive neuron counts, positive neuron counts of unit area and A value of positive cell area: those in experiment No. 1 group: those in experiment No.1 group were higher remarkably than those of control A (P ＜ 0.05) and those in experiment No.2 group were lower remarkably than those of experiment No. 1 group (P ＜ 0.05).CONCLUSION: By controlling hypothalamus SS gene expression and protein expression, Chinese drugs for nourishing yin and reducing fire and benefiting kidney to supplement essence regulate the synthesis and releasing of pituitary growth hormone.

Calcium, as a crucial signal molecule, plays an important role in the development process of immune cells and their functions. After T cell receptors or B cell receptors are stimulated or co-stimulated, the signaling pathways lead to Ca2+ influx and induce the increase of Ca2+ level in cells. Ca2+ signals have different short-time and long- term functions on T and B lymphocytes. Many immune deficiency disorders are resulted from the decrease of Ca2+ influx. This article reviewed the effects of calcium on the functions of T and B lymphocytes and its relationship with some immune deficiency disorders.