Colorimeter detection of Evas Blue in formamide extract of rat tracheal tissue is used as an indication of increased vascular permeability induced by introduction of 115 degrees C air into the rat trachea, followed by a dose (20mg/kg) of meperidine given. The increases of tracheal vascular permeability (TVP) and malondialdehyde (MDA) level induced by hot air injury (HAI) are markedly inhibited. The decrease of superoxide dismutase (SOD) activity induced by HAI is raised. The inhibitory effect of meperidine (MIE) on TVP is not antagonized by naloxone. It is implied that the opiate receptor sensitive to naloxone is not involved in the MIE on TVP. The decrease of tracheal MDA content, increase of tracheal SOD activity raising the ability of scavengering oxygen free radicals and reducing the formation of other active oxygen, inhibit lipid peroxidation. These antioxidative effects may be one aspect of the mechanism of the inhibiory effect of meperidine on TVP.

Objective To study the effect of red blood cells on the secretion of white blood cells modulating globulin in patients with cancer with a new immunological reaction experimental system. Methods Cancer cells (S180, 5?10~6/ml) or 0.2ml of NS or 0.2ml of white blood cells and 0.3ml of plasma were added to anticoagulant suspension of white blood cells treated by citric acid and incubated for 1h at 37℃. The content of globulin (g/L) was determined by Olympus AU100 detector method. 0.2ml of cancer cells was added to 0.2ml of whole blood cells and 0.3ml of plasma in the whole blood experimental group. 0.2ml of NS was added to 0.2ml of whole blood cells and 0.3ml of plasma in the control group. 0.2ml of cancer cells was added to 0.2ml of white blood cells and 0.3ml of plasma in the white blood cell isolation experimental group. 0.2ml of NS was added to 0.2ml of white blood cells and 0.3ml of plasma in the control group. Results Activation rate (0.31?0.09) of globulin in whole blood cell experimental group of patients with cancer was significantly decreased compared to that (0.39?0.14) in normal people. Red cell adhesion rate (0.09?0.08) of globulin in control group was also significantly decreased compared to that (0.29?0.14) in normal people. Conclusion The results of our study indicate that the new experimental system can be used for detection of red blood cell-modulating globulin. The function of red blood cells in modulating globulin of patients with cancer is decreased.

AIM: The changes of myocardial nuclear membrane Ca 2+ -ATPase function was investigated in ischemia/reperfusion injury. METHODS: The model of myocardial ischemia/reperfusion injury was established in rats. Myocardial nuclei were purified with sucrose density centrifugation,the activity of Ca 2+ -ATPase was measured and calcium uptake was assayed with [ 45 Ca 2+ ] . RESULTS: Plasma levels of malondialdehyde (MDA) and free fatty acid (FFA) in myocardial ischemia/reperfusion injury increased significantly( P

Objective To approach the capacity of erythrocyte regulating IL-8 in patients with primary hepatocarcinoma (PHC) by a new experimental system of hemaimmune. Methods 0.2ml suspension of cancer cells (S180: 5?10~6/ml) or NS were added into 0.2ml anticoagulant suspension of whole blood cells or leukocytes and 0.3ml plasma, then incubated for 1 h at 37℃. The content of IL-8 was determined by ELISA. Results In the patients with PHC, the IL-8 levels (pg/ml) in experimental and control groups of whole blood cells, and in experimental and control groups of leukocytes were 376.35?243.96, 353.64?271.92, 461.27?277.11 and 424.97?278.93, respectively; while in the normal human, they were 11.36?6.93, 4.98?4.35, 29.41?30.66 and 20.77?24.20, respectively. In the patients with PHC, the activation rates of cancer cells in the experimental groups of both whole blood cell and leukocyte were 0.22?0.24 and 0.25?0.53, respectively; while in normal human, they were 2.49?2.33 and 0.75?0.21, respectively. In the patients with PHC, the IL-8 adsorption rate of erythrocyte in both experimental and control groups of whole blood cell were 0.22?0.18 and 0.17?0.33, respectively; while in normal human, they were 1.18?2.29 and 0.86?0.49, respectively. The IL-8 activated rates of erythrocyte in the patients with PHC was much lower than in the normal human (P

Objective To determine the effect of antigen on the main immunological reaction route of red blood cells and while blood cells. Methods Cancer cells (5?10~6/ml) and/or Bacillus calmette-Guerin(BCG 0.1mg) or yeast cells(5?10~8/ml) were added into 0.2ml of whole blood cells (or 0.2ml of white blood cells) and 0.3ml of fresh plasma (or 0.3ml of NS) treated by citric acid, and incubated for 1h at 37℃. IL-8 level was measured by ELASA. The data could be divided into 4 groups. (1) 0.2ml of antigen (cancer cells or yeast cells or BCG) was added to 0.2ml of whole blood cells and 0.3m plasma. ②0.2ml of NS was added to 0.2ml of whole blood cells and 0.3ml of plasma. ③ 0.2ml of antigen was added to 0.2ml of white blood cells and 0.3ml of plasma. ④ 0.2ml of NS was added to 0.2ml of white blood cells and 0.3ml of plasma. Results Cancer cells, BCG and yeast cells could activate immunological reaction in blood, but could not activate immunological reaction of white blood cells in no plasma group with addition of antigen. The activation Index (2.124?0.860) of IL-8 in the group with addition of whole blood cells and plasma was significantly higher than that (0.390?0.08) in the group with addition of antigen, white blood cells and plasma (P

Objective To evaluate of red blood cell as giving instruction in whole white blood cell immunological activity by new nature experimental system of hemaimmune reaction rood map. Methods Plasma 0.3ml were added to whole blood cells (including: red blood cell and white blood cells) or white blood cells 0.2ml, and incubated for 1h at 37℃. The content of IL-8 and IL-12 was determined by enzyme linked immunadsorbent assay (ELISA) method. The expression level of CD4, CD8, CD35 and CXCR4 on white blood cells was determined by method of Flow Cytometry. Results The content of IL-8 (5.96?4.26) and IL-12 (9.84?2.23) in whole blood and plasma nature group was significantly lower than that (13.59?3.69?B?pg~ -1 ?ml~ -1 ) and (15.09?9.86?B?pg~ -1 ?ml~ -1 ) in white blood cell and plasma isolation group (P

Objective To investigate the pain level and its influential factors in patients after operation of hepatobiliary surgery. Methods 120 patients were evaluated with the demographic data, State Trait Anxiety Inventory Form (STAI) , Visual Analogue Scale (VAS) , Perceived Social Support Scale (PSSS) and Eysenck Personality Questionnaire (EPQ). Results The highest pain level was within 2h in patients after the operation,then decreased gradually,but its still in the moderate level until 72h after the operation;the pain scores of the PCIA group were lower than the control group ( 2h, 12h after the operation) (P ＜ 0.05, (P ＜ 0. 01 ). The preoperative state anxiety (S-AI) scores were higher than the norm (P＜0. 01 ). The psychoticism Personality characteristics influents the postoperative pain ( 12h,24h after the operation); there were positively correlations between P and VAS (12h, 1week after the operation). The analgesia methods influent the postoperative pain(2h,48h after the operation). The S-AI influents the postoperative pain (2h-48h after the operation) ,there were positively correlations between S-AI and VAS (2 ～ 72h after the operation). The social support influents the postoperative pain (48h after the operation); there were negatively correlations between PSSS score and VAS (48h ,72h and 1 week after the operation). The age and occupation of the patients influent the postoperative pain. Conclusion There was serious pain in patients after operation of hepatobiliary. The preoperative S-AI ,the psychoticism personality characteristics, analgesic modalities, social support, age, occupation of patients are influential factors of pain in patients after abdominal operation.

Objective To study the change in the rule of quick innate immune reactivity of fresh blood corpuscles to cells antigen. Methods Microqrganisms ( Yeast , Escherichia coli , Pseudomanas aeruginosa ) were added in blood anti coagulated by citric sodium and incubated at 37℃ for 30min. Results The innate immune reactivity of the red blood corpuscles to yeast was significantly higher than that of white blood cells ( P

Objective To study the regulatory effects of Ca 2+ , ATP, ADP and AMP on myocardial nuclear Ca 2+ ATPase activity after ischemia reperfusion injury Methods The model of myocardial ischemia reperfusion injury was established in rats Myocardial nuclei were purified using sucrose density gradient centrifugation The activity of Ca 2+ ATPase was measured with ascertaining phosphate Results Compared with the control, plasma levels of MDA and FFA after myocardial ischemia reperfusion injury increased significantly ( P

Aim Observing the alteration of cardiac myocyte nuclear inositol 1,4,5-trisphosphate receptor (IP_3R)binding proterties in rat subjected to myocardium ischemic reperfusion is to make it clear whether this change is involved in the molecule mechanism of cell apoptosis of rat with myocardial ischemic reperfusion. Method Apoptosis index of myocardial cell was determined using TUNEL assay.Extracting of cardiac myocyte nucleus was accomplished by saccharose density gradient centrifugation method,the binding proterties of nuclear IP_3R in different conditions were detected by radioligand binding assay.Results ①Myocardial cell apoptosis index in rat heart underwent 30 min regional ischemia and 3 h reperfusion was distinctly increased compared with sham-operated group(P