ObjectiveTo evaluate the efficacy of Williams life skills training(WLST) on the occupational stress and burnout in the nursing of the intensive care unit ( ICU ).Methods75 female nurses,from ICU of the Second Hospital of Xiangya Medical School,were randomly assigned to the study group( n=37) and the control group(n=38).A 8-week WLST was taken for the nurses in the study group,while the control group did not take training course.All subjects were assessed with nurses working stress questionnaire(NWSQ),Maslach burnout inventory(MBI) before and after the WLST.ResultsAfter 8-week WLST,the score of NWSQ decreased significantly in the study group than that of the control group( (74.09 ± 11.04 ) vs (85.42 ± 13.25 ),P ＜ 0.01 ).The score of the emotional burnout sense of job indifference decreased significantly in the study group than that of the control group((12.33 ±7.21) vs (23.97 ±.46);(3.45 ±.76) vs (7.03 ±.51)) and the score of sense of a-chievement improved obviously in the study group than that of control group( (34.18 ± 5.07 ) vs (29.31 ± 5.75 ),P ＜0.01 ).ConclusionWLST in effective to decrease the occupational stress and burnout level for the ICU nurses.

Objective To explore the factors that influence the quality of life of patients with chronic pain.Methods Using serf-designed questionnaire,simple MiGill pain questionnaire,Beck depression inventory,Beck anxiety inventory,pain self-efficiency questionnaire,athens insomnia scale and SF-12 scale to investigate 114 health people and 139 patients with chronic pain,and analyze the data with analysis of variance and linear regression analysis and path analysis.Results Scores of anxiety(33.56±9.96),depression(17.14±11.22),and insomnia(13.19±6.14)in patients with chronic pain was significant higher(P＜0.01)than the health people (24.55±4.49,24.55±4.49,3.68±3.12 independently),and scores of pain self-control and quality of life(including physical health and mental health)scored lower(P＜0.01)than the health group.Scores of quality of life in chronic pain patients with different pain intensity(F=3.92,P=0.005)and different ache numbers(F=2.39,P=0.041)were significant different,while scores of quality of life in chronic pain patients with different ache frequency was not significant different(F=1.54,P=0.169);quality of life score was significantly correlated(P＜0.05)to the total pain(r=-0.383),the feeling pain(r=-0.315),the emotion pain(r=-0.434),pain selfcontrol(r=0.570),anxiety(r=-0.640),depression(r=-0.523)and insomnia(r=-0.508).Pain self-control,depression,anxiety and insomnia insomnia and depression may indirectly influence the chronic pain patient's quality of life through pain self-control,and insomnia also can affect the quality of life through depression.Conclusion The quality of life in patients with chronic pain was influenced by a number of factors,so it is necessary to take muhi-faceted interventions to improve their quality of life.

Colorimeter detection of Evas Blue in formamide extract of rat tracheal tissue is used as an indication of increased vascular permeability induced by introduction of 115 degrees C air into the rat trachea, followed by a dose (20mg/kg) of meperidine given. The increases of tracheal vascular permeability (TVP) and malondialdehyde (MDA) level induced by hot air injury (HAI) are markedly inhibited. The decrease of superoxide dismutase (SOD) activity induced by HAI is raised. The inhibitory effect of meperidine (MIE) on TVP is not antagonized by naloxone. It is implied that the opiate receptor sensitive to naloxone is not involved in the MIE on TVP. The decrease of tracheal MDA content, increase of tracheal SOD activity raising the ability of scavengering oxygen free radicals and reducing the formation of other active oxygen, inhibit lipid peroxidation. These antioxidative effects may be one aspect of the mechanism of the inhibiory effect of meperidine on TVP.

Objective To systematic evaluate the effectiveness of different psychological treatment methods in the management of postpartum depression (PPD). Methods Relevant literature between 1968 and February 2009 was searched by computer and manual. And suitable randomized controlled trials were brought into this research according to the inclusion and the exclusion criteria. Then meta-analysis was used in four time periods including immediately post- treatment,6 months after treatment, 1 year after treatment and 5 years after treatment.Results A total of eight studies were brought into this research. Immediately post-treatment,it showed significant differences between intervention and control groups of CBT (P = 0.03 ), non-directive counseling(P = 0.0009), psychodynamic therapy (P = 0.005 ) and interpersonal psychotherapy (P = 0.002 ). Within 6 months after treatment, it showed significant differences between intervention and control groups of CBT(P = 0.006 )and peer support (P =0.03) ,but not in non-directive counseling. Within 1 year after treatment,it showed significant differences between intervention and control groups of CBT(P= 0.02 ), but not in non-directive counseling and psychodynamic therapy.Within 5 years after treatment, no significant differences in intervention and control groups of CBT, non-directive counseling and psychodynamic therapy. Conclusions A total of five psychological treatment methods are effective in the management of PPD. CBT has both good short-term and long-term effectiveness. Non-directive counseling and psychodynamic therapy only have a good short-term effectiveness, but a poor long-term effectiveness. Interpersonal psychotherapy and peer support have a good short-term effectiveness.

Objective To explore the efficacy of empathy training on the empathy and emotional intelligence in new employed nurses (engaged in clinical work within one year).Methods A total of 82 participants were randomly divided into the intervention group and the control group (n =41).The intervention group was conducted with empathy training in addition to routine clinical guidance,the control group was only conducted with routine clinical guidance.All subjects were assessed with the Interpersonal Reactivity Index (IRI-C) and Emotional Intelligence Scale (EIS) before and after intervention.Results Immediately after the intervention and 3 months after intervention,the scores of IRI-C in the intervention group were significantly higher than those of the control group((57.53 ±5.20) vs(50.50 ±7.64),(55.28 ±6.48) vs(46.90 ± 7.74),P ＜ 0.01).Samely,the scores of EIS in the intervention group were significantly higher than those of the control group((131.55 ± 10.49) vs(124.35 ±9.93) ;(126.27 ± 12.87)vs(119.38 ±9.45),P＜0.01).Conclusion Conducting empathy training can improve the empathic ability and emotional intelligence of new employed nurses.

Objective To investigate the pain level and its influential factors in patients after operation of hepatobiliary surgery. Methods 120 patients were evaluated with the demographic data, State Trait Anxiety Inventory Form (STAI) , Visual Analogue Scale (VAS) , Perceived Social Support Scale (PSSS) and Eysenck Personality Questionnaire (EPQ). Results The highest pain level was within 2h in patients after the operation,then decreased gradually,but its still in the moderate level until 72h after the operation;the pain scores of the PCIA group were lower than the control group ( 2h, 12h after the operation) (P ＜ 0.05, (P ＜ 0. 01 ). The preoperative state anxiety (S-AI) scores were higher than the norm (P＜0. 01 ). The psychoticism Personality characteristics influents the postoperative pain ( 12h,24h after the operation); there were positively correlations between P and VAS (12h, 1week after the operation). The analgesia methods influent the postoperative pain(2h,48h after the operation). The S-AI influents the postoperative pain (2h-48h after the operation) ,there were positively correlations between S-AI and VAS (2 ～ 72h after the operation). The social support influents the postoperative pain (48h after the operation); there were negatively correlations between PSSS score and VAS (48h ,72h and 1 week after the operation). The age and occupation of the patients influent the postoperative pain. Conclusion There was serious pain in patients after operation of hepatobiliary. The preoperative S-AI ,the psychoticism personality characteristics, analgesic modalities, social support, age, occupation of patients are influential factors of pain in patients after abdominal operation.

Objective:To investigate the demands for cardiac rehabilitation information in patients with coronary atherosclerotic heart disease (CHD) and influential factors.Methods:Information demands for cardiac rehabilitation in CHD patients were surveyed by questionnaire and the influential factors were analyzed by one-way ANOVA and multi-factor analysis of variance.Results:The score of demands for cardiac rehabilitation information in CHD patients was 3.86±0.53.Among them,the most urgent top 5 items were:drug knowledge,diagnosis and treatment,basic knowledge of the heart,emergency and safety and nutrition knowledge.The top 3-demand modes were:communication with medical workers,movies or videos to take home,and lectures.The score of demands for cardiac rehabilitation information was different in different age groups.The highest score was in the patients with age less than 60.There were different demands in different characteristic groups.Conclusion:The most urgent need and mode are drug knowledge and communication with medical workers,respectively.With the age increase,the demands for patients' cardiac rehabilitation information decrease.An individualized health education strategy should be developed according to the characteristics of CHD patients.

Objective:To explore the relationship between social support and negative emotion in parents of children with congenital heart disease before the operation.
Methods:Social support rating scale (SSRS), self-rating anxiety scale (SAS), and Center for epidemiological survey-depression scale (CES-D) were used to assess the association between social support and anxiety and depression in 226 parents.
Results:The mean SSRS score was signiifcantly lower than the normal. There was signiifcant difference in social support of parents in monthly family earning and knowledge about surgery. Parents with monthly family income≤1000 yuan had lower scores than the other groups. Parents who knew nothing about the surgery approach had lower scores. Social support was significantly correlated to negative emotion (anxiety and depression). Social support could predict anxiety and depression.
Conclusion:Social support is widely and significantly correlated to negative emotion in parents of children with congenital heart disease before the surgery.

Objective To conduct a methodological evaluation on flowcytomotry fluorescence detection of serum CA242, and to evaluate its value in the diagnosis of pancreatic cancer.Methods The blood samples of 40 cases of pancreatic cancer, 49 cases of other tumors, 48 cases of benign digestive diseases and 159 healthy volunteers were collected.Fowcytometry fluorescence immunoassay was used to detect serum level of CA242, and it was compared with routine ELISA method to measure its sensitivity and specificity.Results The detection limit of CA242 by flowcytometry fluorescence immunoassay was 0.89 U/ml, the linear range was 1～ 500 U/ml after confirmation.The within-batch CV was 3.37％～ 5.30％, between batch CV was 7.43％ ～ 9.60％.When compared with routine ELISA, flowcytometry fluorescence immunoassay showed the equation of linear regression is Y =1.0398X-0.947, r =0.9687.Area under ROC curve was 0.811 ± 0.025 (95％ CI 0.763 ～0.859), with 18.625 U/ml as the best cutoff value, the specificity was 92.0％ and the sensitivity was 62.1％.Conclusions Flowcytometry fluorescence immunoassay for CA242 testing has the advantage of shorter detection time, miltiple sample and testing project detection, which is worth of clinical application.

AIM: To investigate the changes in nuclear calcium content and permeability of nuclear pore complex in rat myocardium during ischemia reperfusion injury.METHODS: The rat model of myocardial ischemia reperfusion injury was established.Myocardial nuclei were purified using sucrose density gradient centrifugation.The nuclear calcium content was measured by atomic absorption spectrophotometer.The permeability of nuclear pore complex was assessed through measuring the amount of calmodulin conjugated Alexa Fluo~(TM) 488 as fluorescent probes transported across nuclear membrane with spectrofluorometer.RESULTS: The nuclear calcium content at 15,30,60,120 and 180 min reperfusion following 30 min sustained ischemia increased 1.31-,1.55-,1.73-,1.94-and 2.14-fold,respectively,as compared with sham-operation group.The permeability of nuclear pore complex at 15 min reperfusion following 30 min sustained ischemia showed no difference from sham-operation group,but it only increased 1.31-,1.38-,1.40-,and 1.48-fold at 30,60,120 and 180 min reperfusion following 30 min sustained ischemia compared with sham-operation group.CONCLUSION: The nuclear calcium content during myocardial ischemia reperfusion injury increases earlier than the permeability of nuclear pore complex does.The increase in the permeability of nuclear pore complex may result in adaptive regulatory effects on nuclear calcium overload to a certain extent during myocardial ischemia reperfusion injury.