Objective:To study the effect of alum on immune response in mice induced by HBoV1 VP2 VLPs.Methods:BABL/c mice were randomly divided into VLPs experimental group, alum adjuvant experimental group, PBS control group and alum adjuvant control group,the experimental group mice were intramuscular immunization with HBoV1 VP2 VLPs and HBoV1 VP2 VLPs added alum,control group mice were immunization with alum or PBS buffer,then to study the effect of alum on immune response in mice induced by HBoV1 VP2 VLPs by cellular and humoral immune strength.Results: Alum adjuvant decreased cellular immune response induced by HBoV1 VP2 VLPs(P<0.001),enhance the HBoV1 VP2 VLPs immuned serum IgG titer(P<0.05)and IgG activity(P<0.01).Conclusion:Alum adjuvant can enhance humoral immune response induced by HBoV1 VP2 VLPs,but weaken cellular immune response induced by HBoV1 VP2 VLPs,when HBoV1 VP2 VLPs used as a prophylactic vaccine it should add alum adjuvant,when used as a therapeutic vaccine,it should not add alum adjuvant.

OBJECTIVE: To investigate a revision of the Trauma and Injury Severity Score (TRISS) weight coefficients in order to overcome the inference from foreign coefficients on Chinese trauma scoring. METHODS: The data of 1 297 Chinese trauma patients were studied for trauma scoring with the Revised Trauma Score-Injury Severity Score-TRISS (RTS-ISS-TRISS) system to get a serial of new weight coefficients through analyzing a multivariation logical regression between the outcome and the injury severity. RESULTS: ISS was higher but the Age Score and probability of survival (Ps) of the death group were lower than those of the survival group. New values of RTS-ISS-Age coefficients differed from the Major Trauma Outcome Study (MTOS) ones, through which the constant b(0) decreased its negative value, and ISS weight b(2) increased its negative value, but RTS weight b(1) and age weight b(3) changed with the trauma types. MTOS's values and new values of weight coefficients were used on 1297 patients for prognosis by calculating Ps. The accuracy of new values (90.13%) was a little higher than that of MTOS's (89.5%), with a promotion in specialization but a loss in sensitivity. CONCLUSIONS: A revision of TRISS's weight coefficients is either necessary or feasible. To achieve this purpose, a Chinese trauma database with large capacity is recommended.