Objective]Explore the Neijing·Lingshu and channels and col aterals theory of traditional Chinese medicine on root end, the meaning of the appearance substance, the development and application.[Methods]Through consulting relevant literature, the paper expounds root end, appearance substance in channels and col aterals theory, the definition and connection, and the root knot, appearance substance and qi and blood, specific hole, gas street, universal and systematic review of clinical application, etc. [Results]Root end is similar but not identical and appearance substance theory, root end heavy concrete location, up and down on both ends of the appearance substance weight area. Qi by no out, a way to root, slip, note, and five acupoints, the head of the parts to the auction, offering acupuncture point, back-shu point, the acupuncture point, etc., in accordance with the gas tract, universal distribution position. Come a long way to Selecting distant points and root-pass-knot match point, provide theoretical basis for clinical application. [Conclusions]Root end, appearance substance theory is a supplement to the theory of meridians and improvement of the clinical syndrome differentiation, finding out the formula and specific point one of the clinical application provides the theoretical methods, the application method is an extension of the concept on also to expand scope provided theoretical guidance for the treatment of disease. The disease diagnosis and treatment have important significance.