OBJECTIVE To study the organism and drug resistance in chronic obstructive pulmonary disease(COPD) patients with Gram-negative bacteria(GNB) nosocomial pneumonia,in order to provide the index to clinician. METHODS The bacteria were tested by Micro WalkAway-40 which made in the USA,the susceptibility test was carried by disk diffusion test. RESULTS The rates of Klebsiella pneumoniae and Pseudomonas aeruginosa among all bacteria were higher,which were 26.8% and 26.2%,respectively;the organisms were almost drug resistant,which were more sensitive to imipenem,amikacin and ceftazidime,and more resistant to other drugs. CONCLUSIONS The hospital should make precautions,diagnose in time,and use antibiotic reasonably.

OBJECTIVE To investigate nosocomial infection and drug-resistance of Pseudomonas aeruginosa(PAE) in our hospital to provide the evidence for clinical monitoring and control of nosocomial infection.METHODS The microbiological system MicroScan WalkAway-40 was used to identify PAE and the drug-resistance in vitro was determined by K-B methods.RESULTS Among 325 strains of PAE in two years,imipenem-resistant P.aeruginosa(IRPA) accounted for 14.8%.The highest isolating rates occurred in the intensive care unit(ICU),accounted for 20.9%.The following was senile disease department,accounted for 19.4%.The respiratory department rated the third,accounted for 16.9%.The resistance of IRPA to 11 kinds of common antibiotics was significantly higher than imipenem-sensitive P.aeruginosa(ISPA).Except the drug-resistance of IRPA to ceftazidime,cefepime and aztreonam was below 50.0%,that to the other eight antibiotics was all over 50.0%.CONCLUSIONS The drug-resistance of PAE is very serious in our hosptial.We should perform rigorous monitoring and prevent the outbreak of nosocomial infection with PAE.

Objective To compare the characteristics,complications and outcomes of the modified lapa-roscopic Swenson(MLSw)and laparoscopic Soave(LS)procedures for children with short-segment Hirschsprung disease(HD). Methods Seventy-seven pediatric patients with HD who underwent surgery from March 2007 to December 2016 were enrolled in this retrospective study. Twenty-six patients were treated with LS and 51 cases un-derwent MLSw. The preoperative,operative and postoperative data was collected,with follow-up periods ranging from 12 to 48 months. The perioperative/operative characteristics,postoperative complications,and outcomes were compared between the two groups. Results On average,the patients in the LS group had a longer operating time than that in the MLSw group(P < 0.05). Blood loss was significantly less in the MLSw group than that in the LS group(P < 0.05). There was no significant difference in feeding time between the two groups(P > 0.05). The MLSw group was discharged after a shorter hospitalization time than that in the LS group(P < 0.05). The MLSw group had lower incidences of postoperative complications than those in the LS group in the early postoperative period,with no significant difference in the rate of complications during the late postoperative period was found between the two groups. Conclusions Both LS and MLSw are suitable for treatment of children with short-segment HD. However,the MLSw operation is much simpler,with less operating time,less intraoperative blood loss,shorter hospitalization time and better bowel control in the early postoperative period. We favor this approach because it allows complete removal of the entire original aganglionic bowel,without leaving behind a cuff.