1.Diagnosis and Treatment of 12 Cases with Uncommon Pulmonary Sequestration
Mingzhao WANG ; Ying ZHANG ; Yi SHEN ; Lesheng LIN ; Yiren LUO
Journal of Kunming Medical University 1990;0(02):-
Objective To explore the diagnosis and surgical treatment of pulmonary sequestration.Methods 12 patients of pulmonary sequestration were retrospectively studied.Results Between January 2002 and December 2005 12 patients with pulmonary sequestration were operated in our department.8 patients were diagnosed preoperatively by chest enhanced CT which showed an abnormal feeding artery.4 patients were misdiagnosed.10 cases of intralobar were performed lobectomy,2 cases of extralobar were resected the separated lung tissue.No death occurred in our group.Conclusion Enhanced CT scan is a major diagnostic method of pulmonary sequestration and operation can obtain excellent results.
2.Expressions of endothelin-1 in lung cancer and metastatic lymph nodes and clinical significance
Qingfeng CHEN ; Wenjie JIAO ; Lesheng LIN ; Xianlu SUN ;
Journal of Third Military Medical University 2002;0(12):-
3 cm in diameter was 65.6%, while the rate in those
3.Promoter methylation status and protein expression of p14ARF gene in squamous cell carcinoma and adenocarcinoma of the lung.
Kaihua TIAN ; Lesheng LIN ; Zhaotong JIA ; Xiaojing GUO ; Lin ZHANG
Chinese Journal of Lung Cancer 2006;9(1):40-44
BACKGROUNDRecently, the p14 ARF gene has emerged as a new putative tumor suppressor gene, and the alteration of p14 ARF gene is closely related to development of multiple human tumors. The aberrant promoter methylation as a mechanism of inactivation of p14 ARF gene might participate in tumorigenesis. The aim of this study is to investigate promoter methylation status and protein expression of p14 ARF gene in pulmonary squamous cell carcinoma and adenocarcinoma, and to value the role of p14 ARF promoter methylation in carcinogenesis of non-small cell lung cancer.
METHODSPromoter methylation status and protein expression of p14 ARF gene were analyzed in 40 cases of pulmonary squamous cell carcinoma and adenocarcinoma by methylation specific polymerase chain reaction (MSP), restriction enzyme-related polymerase chain reaction (RE-PCR) and immunohistochemistry (IHC).
RESULTSThe positive rate of p14 ARF promoter methylation in tumor tissues and normal tissues adjacent to cancer was 17.5% (7/40) and 2.5% (1/40) respectively (P= 0.025 ). The results of RE-PCR were consistent with the above. The positive rate of p14 ARF protein in tumor tissues was significantly lower than that in normal tissues adjacent to cancer (P=0.003). Promoter methylation and protein expression of p14 ARF gene showed a significantly negative correlation (r=-0.56, P= 0.001 ), and both of them did not correlate statistically with the clinicopathologic characteristics of patients such as histological classification, TNM stages, differentiation grade and lymph node involvement.
CONCLUSIONSPromoter methylation is a crucial mechanism of inactivation of p14 ARF gene. Promoter methylation of p14 ARF gene might be involved in carcinogenesis of non-small cell lung cancer, and it is an early event in development process of non-small cell lung cancer.