Objective To identify and characterize the CD34~+ stem cells of hematopoietic tumor cell lines. Methods Taking CD34 molecule as a marker to identify if there are CD34~+ tumor cells in hematopoietic tumor cell lines(THP-1,MEL,K562,MOLT-4,RAJI,RPMI8226,SP2/0 and DCS) by fluorescence staining and FCM technology. We separated CD34~+ cells from K562 and MOLT-4 for further study.CD34~+ cells were cultured and observed their development.CD34~+ and CD34 +- cells were compared in their functional status(mRNA),cell cycles(G -0/G -1),their degree of differentiation(Id-1 expression) and their multi-drug resistance(gp expression). Results The percentages of CD34~+ cells were 6 ^2%(DCS),5.2%(MEL),1.4%(RAJI),2.5%(THP-1),2.1%(MOLT-4),3.1%(K562),0(SP2/0),and 0(RPMI8226) respectively.After culturing CD34 ++ cells isolated from K562 cells line could proliferate and the percentage of CD34 ++ reminded the same.CD34 ++ tumor cells contained much less RNA,more G0/G1 cells,expressed more Id-1 protein(less differentiated state) and possessed more P-gp(stronger multi-drug resistance).Conclusion There exists a very small portion of CD34 ++ stem cells among hematopoietic tumor cells and these cells are in a more primitive state.

Objective To investigate the effects of music therapy in treating cognitive dysfunction in early stroke patients. Methods Forty patients who had suffered a stroke in the previous four weeks were randomly di-vided into two groups of 20. One received music therapy as part of their cognitive training ( observation group) , and the other did not (control group). Before and after 6 weeks of treatment, cognitive functioning was evaluated using the second Chinese edition of Loewenstein's occupational therapy cognitive assessment (LOTCA). The patients' a-bility in the activities of daily living was also evaluated using the modified Barthel index (MBI). Results After treatment, the total LOTCA and MBI scores in both groups were significantly higher than before. Both LOTCA and MBI scores in the observation group were significantly higher than in the control group, and there were also signifi-cant differences between the observation and control groups on all of the LOTCA sub-items except spatial percep-tion. Conclusions Music therapy can improve the effects of rehabilitation on cognition and the ADL ability of early stroke patients.