A total of 13 allozymes of 7 populations of Oncomelania snails trom 7 provinces in the mainland China were examined by horizontal starch gel electrophoresis. Results demonstrated that out of 17 loci examined, 10 were monomorphic loci: Aldh,Ao,G6pd ,Gpdh,Hbdh,Ldh, Sdh,Est-2,Est-3,and Est-6,and 7 were polymorphic loci:Est-4,Est-5,Xdh,Mdh,Idh,Got and Ap. Banding pattern showed that six enzymes were polymorphic accounting for 46.15 % (6/13) of total enzyme systems examined. Results indicated that a certain of relative relation-ship was existed along with the significant variation presented based on their environment and location among the 7 populations of snails from the mainland of China. It was revealed that the 7 polymorphic loci were the important loci affecting the gentic variation in the differ-ent populations of Oncomelania spp.

Objective To investigate the effects of left dorsolateral prefrontal cortex with 10HZ , 80%motor threshold repetitive transcranial magnetic stimulation ( rTMS ) on the cognitive function in unipolar depression. Method Sixty-nine patients with unipolar depression were randomly assigned to either the real rTMS group or the sham rTMS treatment( the control group) ,all patients were given extended releasing venlafaxine. the cognitive function was evaluated using P300 and Repeatable battery for the assessment of neuropsychological status ( RBANS-immediate memory, visuospatial, language, attention, delayed memory, total score), Wisconsin card sorting test ( WCST-Right responses, Completed categories, Total errors , Preservative errors, Nonpreservative errors ). The depression severity was assessed with the 24-item Hamilton Rating Scale for Depression(HAMD-24). All of tests were examined before and after six weeks with thirty times rTMS. Results At the end of six weeks treatments, regarding the WCST,real rTMS group showed better improvement in the right responses than control group(33.23±10.29 vs 27.09 ± 9.82, F= 16. 116 , P= 0. 000), besides right responses, real rTMS group had better performance in the rest items than control group(F=4.862 ～ 17.758, P= 0.031 ～ 0.000) ;concerning the RBANS, real rTMS group was significantly superior to control group in total score( (88.83 ± 16.48 ) vs (78.85 ± 13.51 ), F= 8. 425,P = 0. 005 ), besides total score , the real rTMS group had better performance in some rest factors than control group (F= 10.088 ～20. 801, P=0. 002 ～0.000);real rTMS group showed better improvement in the amplitude of P3than control group( (8.27 ± 2.97 ) μV vs ( 7.37 ± 2.66) μV, F= 5. 838 , P=0.018 ) ;real rTMS group demonstrated better improvement in HAMD-24 than control group( (4.7 ±2.4)vs ( 11.2 ±5.1 ), F= 29.537, P=0. 000).Conclusion rTMS can significantly improve cognitive function and depressive symptoms with unipolar depression.

Objective To study the liver fat content (LFC) in schizophrenia patients during olanzapine or aripipra?zol treatment, and to explore the relation between LFC and insulin resistance (IR). Methods Schizophrenia patients were randomly administered with olanzapine (10~25 mg/d, n=57) or aripiprazole (15~30 mg/d, n=47) for eight weeks. All sub?jects underwent sonographic quantification of LFC and homeostasis model assessment of insulin resistance index (HOMA-IR) once 0, 4, 8 weeks of treatment. Results Compared with baseline, the levels of HOMA-IR significantly in?creased after a 4-week and an 8-week of olanzapine treatment, and so did the LFC after an 8-week of olanzapine treat?ment (P<0.05). The levels of LFC (P>0.05) or HOMA-IR (P>0.05) did not significantly changed at week 4 and 8 in ar?ipiprazol group. The increment of LFC, HOMA-IR at week 8 was significantly higher in olanzapine group than that in ar?ipiprazol group (P<0.05). The change of LFC after 8-weeks olanzapine treatment was positively correlated with the change of HOMA-IR (r=0.298, P=0.036). Conclusion Olanzapine treatment increases whereas aripiprazol has little ef?fect on liver fat and insulin resistance in schizophrenia.

In allusion to high labor intensity, low working efficiency and high error rate in hospital laboratory, a detection system of the enzyme-linked analyzer based on Multiscan EX instrument is developed. Being easy to operate, the detection system proves effective in enhancing working efficiency. It deserves popularizing because of its comprehensive applications to the detection.

Objective To explore the method of Oncomelania hupensis snail surveillance in transmission?interrupted re?gions of schistosomiasis,so as to provide a new pattern for the surveillance of snail status in the transmission?interrupted areas. Methods In Dongtai County,north of Jiangsu Province where the transmission of schistosomiasis was interrupted,the surveil?lance of snail status was performed by means of the village?level general survey,village?level sampling survey,snail survey in key settings of township and county,and snail reporting by residents. In addition,quality?control snail sites were set up for quali?ty control. The effectiveness and cost of the four methods of snail surveillance was compared. Results A total of 163 079 set?tings and 22 785.62 hm2 were surveyed in Dongtai County from 2008 to 2013,and 89 residual snail breeding sites were found, with a snail area of 1.48 hm2. The patterns of village?level general survey,village?level sampling survey,and snail survey in key settings of township and county were employed for investigation of 94 550,45 033 and 23 496 settings,consisting of 57.98%, 27.61%and 14.41%of total settings,and 2,0 and 6 settings were found with snails,with 0.021/thousand,0,and 0.255/thou?sand detection rates of settings with snail breading sites,respectively. The pattern of snail survey in key settings of township and county was more effective than that of village?level survey to detect snail breeding sites(χ2 =19.158,P=0). The recovery rate of quality?control snail breeding sites was 52.56%,38.27%and 73.62%for the three patterns of snail survey,respectively,and the pattern of snail survey in key settings of township and county showed a higher quality for snail survey than that of village?lev?el survey(χ2 =111.597 and 85.991,both P=0). During the period from 2008 to 2013,289 person?times reported 279 sus?pected snail habitats and 1 501 living snails,and no Oncomelania snails were found. During the 6?year period,there were 1617.5,964.7 and 527.7 thousand RMB invested for village?level general survey,village?level sampling survey,and snail sur?vey in key settings of township and county,and the cost of snail survey per hm2 was 129.88,133.6 and 162.57 RMB/hm2,re?spectively. The cost of village?level general survey and snail survey in key settings of township and county to detect a snail?breed?ing site was 808 800 and 88 000 RMB,respectively(t=12.850,P=0.000),and the cost of snail survey in key settings of town?ship and county was 10.88%of that of village?level general survey. Conclusion The snail survey in key settings of township and county is a highly effective and high?quality method for snail surveillance,which may serve as a prior way for the surveil?lance of snail status in the transmission?interrupted areas of schistosomiasis.

Objective To explore the spatial distribution of schistosomiasis cases and Oncomelania snails in the provinces of Jiangsu, Anhui and Jiangxi with the assistance of geographic information systems (GIS). Methods Epidemiological data of schistosomiasis in recent 20 years were collected, and the relevant GIS databases were established. The spatial autocorrelation analysis was carried out with ArcView 3\^x, S\|Plus, and Spatial Statistics software. Results Except for schistosomiasis cases in Jiangsu, the spatial distribution of schistosomiasis cases and Oncomelania snails in the three provinces were fitted to the spatial autocorrelation at a certain degree. Generally the autocorrelation coefficients (Moran′s I) in Oncomelania snails were higher than that in schistosomiasis cases. Conclusion Spatial autocorrelation analysis can be used to estimate the value of one variable in different spatial position, and it is a good way to find out the spatial cluster distribution in different stages.

Objective To understand the distribution,variance and dynamics of Oncomelania snails in the water system of the Bianmin River after the river bank being protected with concrete.Methods The snails in the water system of the Bianmin River were investigated with the systemic sampling method and environmental spot-checking method in spring from 2002 to 2005.The indexes including area with snails,area with infected snails,rate of frames with snails,density of living snails and infection rate of snails in different environments were collected,analyzed and compared year by year.Results From 2002 to 2005,in the main river of the water system of the Bianmin River,irrigation areas and the adjacent marshlands of the Yangtze River,the areas with snails were 20.94-31.88,39.30-48.08 hm2 and 275.13-292.31 hm2,respectively;the rates of frames with snails were 9.96%-21.88%,29.61%-58.54% and 62.14%-85.97%,respectively;the mean densities of snails were 0.97-1.86,0.92-3.96 snails/0.1 m2 and 7.72-11.15 snails/0.1 m2.In 2005,in the main river,irrigation areas and the adjacent marshlands,the rates of frames with snails were 21.88%,39.33% and 83.81%,respectively;the densities of living snails were 1.30,3.96 snails/0.1 m2 and 11.15 snails/0.1 m2.During the last four years,the rates of frames with snails increased quickly in the main river of the water system of the Bianmin River;the densities of snails increased by 4 times in the irrigation areas;the indexes mentioned above were stable in the adjacent marshlands of the Yangtze River.However,the rate of frames with snails in the marshlands was 2-3 times higher than that in the irrigation areas and the mean density of snails in the marshlands was 3-8 times higher than that in the irrigation areas.Conclusions The main factor causing the increase of snails is that the river beach has not been cleaned yet in the water system of the Bianmin River,and so the key of schistosomiasis control is that first,the river beach should be cleaned completely,and at the same time,strengthening the snail control in the irrigation areas and the snail surveillance and snail control with molluscicides in the outfalls of the rivers of the water system of the Bianmin River to the Yangtze River.

Objective To evaluate the mid-term effectiveness of the medium-and-long-term programme of the prevention and control of schistosomiasis in Jiangsu Province. Methods The programme was carried out in Jiangsu Province and the comprehensive measures relied mainly on snail control, detection and chemotherapy of schistosomiasis for residents and livestock. The mid-term effectiveness of the programme was observed and evaluated longitudinally. Results The coverage rate of snail control areas and the ratio of chemotherapy of schistosomiasis for residents and livestock rose year after year. The snail areas, the infected snail areas and the infection rate of schistosome for residents and livestock reduced year after year. There were negative correlations between the coverage rates of snail control and the infected snail areas, the number of settings with infected snails, the infection rate of schistosome for residents, the infection rate of schistosome for livestock and the morbidity of acute schistosomiasis, respectively (rInfected snail areas=-0.897,P=0.039;rNo. of settings with infected snail=-0.937,P=0.019;rInfection rate for residents=-0.913,P=0.031;rInfection rate for livestock=-0.991,P=0.001;rMorbidity of acute schistosomiasis=-0.894,P=0.041). There were negative correlations between the ratio of chemotherapy of schistosomiasis for residents and the infected snail areas, and between the ratio of chemotherapy of schistosomiasis for livestock and the infected snail areas (rRatio of chemotherapy for residents =-0.965,P=0.008;rRatio of chemotherapy for livestock=-0.921,P=0.027). There were negative correlations between the funds of schistosomiasis control and the infection rate of schistosome for residents livestock, respectively (rInfection rate for residents=-0.897,P=0.039;rInfection rate for livestock=-0.971,P=0.006). The multiple linear regression analysis showed that there were the linearity relationships between the infection rate of schistosome for residents and the coverage rates of snail control, and between the infection rate of schistosome for livestock and the coverage rates of snail control, and between the morbidity of acute schistosomiasis and the coverage rates of snail control. Conclusions The effect of the comprehensive strategy of schistosomiasis control which relies mainly on snail control, detection and chemotherapy of schistosomiasis for residents and livestock are remarkable. The snail control and chemotherapy are effective and immediate. The increased funds would be essential guarantee for control and elimination of schistosomiasis.

Objective To explore the effect of repetitive transcranial magnetic stimulation(rTMS) treatment on the brain derived neurotrophic factor(BDNF) serum levels in depressive patients.Methods Sixty-eight unipolar depressions treated with venlafaxine were randomly assigned to the real rTMS group(n =34)and the sham rTMS group(n =34),which were accepted the real or the shame rTMS treatment on the left dorsolateral prefrontal lobes respectively.The Hamilton Rating Scale for Depression (HAMD) and BDNF serum was assayed before and after 4 weeks' treatment.Results 1) A significant increase of serum BDNF((12.2 ± 1.3) μg/L vs (5.6 ± 0.8) μg/L,t=-9.167,P=0.000;(11.4 ± 1.5)μg/L vs (6.0± 1.0)μg/L,t=-7.421,P=0.000)and a significant decline of HAMD((11.6 ± 1.7) score vs (32.6 ± 2.5) score,t =14.654,P =0.000 ; (4.2 ± 2.8) score vs (31.8 ± 3.2)score,t=12.089,P =0.000) were found after the treatment in the real and the shame group,and the real group changed more significantly than the shame group ((6.7 ± 0.8) μg/L vs (5.1 ± l.2) μg/L,t =2.690,P =0.009 ; (21.0 ± 2.1) score vs (17.6 ± 2.6) score,t =2.693,P =0.000).2) A negative correlation was found between the serum BDNF levels and the HAM D scores before the treatment(r =-0.530,P=0.003; r =-0.490,P =0.004),and a positive correlation between changes of BDNF levels and HAMD scores changes(r =0.439,P =0.006 ; r =0.454,P =0.005).Conclusion The rTMS treatment can increase serum BDNF levels in depressive patients.

The genetic divergence between geographically separated populations can be studied by comparing the divergences in their allele frequencies to the geographical distance of popula-tions. The present study is to examine the divergences in allele frequencies and genetic diver-gence in relation to the geogrephical distance of the samples localities, in order to test the population structure model of Oncomelania from the endemic areas in mainland China.The results showed that three patterns of allele frequency distributions occured in seven polymorphic loci, which were even,chaotic and discontinuous cline distributions. The distcon-tinuous or stepped clines shown in loci Est-4,Got and Mdh-2 suggested that the discrete subpopuation model is the likeliest. The plot of genetic distance (Nei's 1978) between sample populations against the geographic distances suggested that the special pattern of allele fre-quency distribution could be found. The regression analysis shows that logistic sigmoid re-gression is the best model to fit the original data in the plot. This supports the existence of the discrete subpopulations model in the population structure studied.