Objective To explore the application effect of holistic nursing conception in teaching ward-round.Methods From May 2015 to April 2016, totally 112 internship nurses of undergraduate and junior college of Xiaolan Affiliated Hospital of Southern Medical University were included in this study. The students were randomly divided into the observation group and the control group, with 56 people in each group. In the observation group, nursing students were regarded as the main body, holistic nursing concept was applied in nursing teaching ward-rounds. While in the control group, teachers were regarded as the main body in teaching ward-rounds. Teaching effects were compared between two groups.Results The following indexes were observed. Nursing assessment and nursing experience, evaluation of interns ability gaven by teachers (including holistic care ability, ability to analyze and solve questions, ability of literature review, critical thinking skills, integrity and fluency when reporting the patient history, ability to find the key problems), interns' self ability evaluation (including improving clinical communication ability, improving the ability of analyzing and resolving problems, improving the critical thinking skills, improving the ability of the literature review, holistic care ability, stimulate interest in learning, promoting the attraction of teaching rounds, degree of internship harvest).The results showed that the observation group were better than the control group (P<0.05).Conclusions Application of holistic nursing concept in teaching ward-round can stimulate the learning enthusiasm of nursing interns, and improve holistic care ability and clinical ability of analyzing and resolving problems.