No abstract available.
Hyperplasia* ; Leishmania donovani* ; Leishmania*

No abstract available.
Hyperplasia* ; Leishmania donovani* ; Leishmania*

No abstract available.
Bone Marrow ; Leishmania ; Leishmania donovani

No abstract available.
Bone Marrow ; Leishmania ; Leishmania donovani

Female patient, 29 age, married, lives in Ha Long. Admitted of hospital 16/03/2001. Reason for admitted hospital: prolonged fever symtoms: splenomegaly, hepatomegaly, enlargement of the lymph glands, anemia, purpura. Investigation: thrombocytopenia, hypohemoglobin, hyperglobulin, HIV positive. Hypermacrophages were indentidied by microsscopy in the bone marrow smear: parasites in macrophages look like Amastigote of Leishmania parasites in the atlas (the morphology of human blood cells- abbott laboratories). Born marrow cultured in NNN medium: identidied promastigote. Diagnosis: visceral Leishmaniaxsis. It was confirmed by Karolinska hospital of Sweden, China, Singapore, Australia. This is first case indentified in Vietnam
Leishmaniasis, Visceral ; Leishmania donovani

A female patient 29 years old, married, lived in Halong was admitted to hospital in 16/03/2001. Reason for admitting hospital: prolonged fever. Symptoms: splenomegaly, hepatomegaly, enlargement of the lymph glands, amenia and purpura. Investigation: Thrombocytepenia, hypomemoglobin, hyperglobulin, HIV positive. Hypermacrophages were identified by microscopy in the born marrow smear: parasites in macrophages look like Amastigote of Leishmania parasites in the Atlas (The morphology of human blood cells- Abbott laboratories). Born marrow cultured in N.N.N medium: identified Promastigotes. Diagnosis: Visceral Leishmaniasis. It was confirmed by Karolinska hospital of Sweden, China, and Singapore. This is first case identified in Vietnam
Leishmaniasis, Visceral ; Leishmania donovani

A new minicircle in the mitochondria genome of Leishmania mexicana M379, clone E8 was monitored using different methods as PFGE, hybridization,... The 0,7-kb of the circle was cloned and analysed. It appears to have a Chi-like octomer in the region of replication
Mitochondria ; Leishmania mexicana

A mitochondria minicircle in Leishmania mexicana M379, clone evans E8 was cloned and characterized. The molecules exist as dominant class in the mitochondria genome of the parasiters.
Mitochondria ; Leishmania mexicana

A total of 1,236 people from the villages of Ha Phong (Ha Long city), Cam Tay, Cam Son (Cam Pha town) and some peripheral villages were examined for blood flagellata Leishmania. 377 people were found to have clinical symptoms of prolonged fever, cough, inflamed gland, hepatomegaly or skin infection. 65 cases had been previously confirmed to be HIV carriers. Peripheral blood was collected from 337 people and bone marrow samples from 229 people examined for Leishmania were negative. Analysis made with 496 slides from 170 local dogs, 120 slides of bone marrow, liver, spleen from 10 dogs, and 900 slides of bone marrow , liver, spleen from 100 rats (Rattus flavipectus, R. norvegicus) also showed negative results. 104 sand flies were collected in Ha Phong village and were identified Phlebotomus (Anaphlebotomus) stantoni where one patient was found in 2001
Leishmania ; Epidemiology ; Blood

Despite the broad distribution of leishmaniasis among Iranians and animals across the country, little is known about the genetic characteristics of the causative agents. Applying both HSP70 PCR-RFLP and sequence analyses, this study aimed to evaluate the genetic diversity and phylogenetic relationships among Leishmania spp. isolated from Iranian endemic foci and available reference strains. A total of 36 Leishmania isolates from almost all districts across the country were genetically analyzed for the HSP70 gene using both PCR-RFLP and sequence analysis. The original HSP70 gene sequences were aligned along with homologous Leishmania sequences retrieved from NCBI, and subjected to the phylogenetic analysis. Basic parameters of genetic diversity were also estimated. The HSP70 PCR-RFLP presented 3 different electrophoretic patterns, with no further intraspecific variation, corresponding to 3 Leishmania species available in the country, L. tropica, L. major, and L. infantum. Phylogenetic analyses presented 5 major clades, corresponding to 5 species complexes. Iranian lineages, including L. major, L. tropica, and L. infantum, were distributed among 3 complexes L. major, L. tropica, and L. donovani. However, within the L. major and L. donovani species complexes, the HSP70 phylogeny was not able to distinguish clearly between the L. major and L. turanica isolates, and between the L. infantum, L. donovani, and L. chagasi isolates, respectively. Our results indicated that both HSP70 PCR-RFLP and sequence analyses are medically applicable tools for identification of Leishmania species in Iranian patients. However, the reduced genetic diversity of the target gene makes it inevitable that its phylogeny only resolves the major groups, namely, the species complexes.
Animals ; Genetic Variation* ; Humans ; Iran ; Leishmania infantum ; Leishmania major ; Leishmania tropica ; Leishmania* ; Leishmaniasis ; Parasites* ; Phylogeny ; Sequence Analysis*