Objective To explore effect of fluorochloridone on primary co-cultured sertoli-germ cell of rat and its possible mechanism.Methods primary co-cultured sertoli-germ cell was made by two steps of enzyme digestion with SD rat testes,after 24h of Sertoli-germ cell isolation,A 0.1％ DMSO solvent control group and three FLC exposure groups (10-6、10-7、104 mol/L) were selected,cultured cell for 24h,then MTr assay and index detection of oxidative stress were performed.Results The mortality of primary co-cultured sertoli-germ cell exposedto 10-6mol/LFLC was significantly higher than controlgroupandFLC exposure groups (10-7、10-8mol/L) (P＜0.05).10-6mol/L FLC reduced enzyme activity of CAT,SOD and GSH.Px,depressed GSH level,elevated MDA level,and had significant difference than control group and FLC exposure groups (10-7、10-8 mol/L) (P＜ 0.05).10-7 mol/L FLC decreased enzyme activity of CAT than control group,depressed enzyme activity of SOD than 10-8 mol/L FLC,lowered GSH level than control group and FLC exposure groups (10-8 mol/L),and had statistical difference (P＜0.05).Conclusion FLC can damage primary co-cultured sertoli-germ cell of rat,its possible mechanism is relevant to oxidative stress.

Objective To explore effect of fluorochloridone on primary co-cultured sertoli-germ cell of rat and its possible mechanism.Methods primary co-cultured sertoli-germ cell was made by two steps of enzyme digestion with SD rat testes,after 24h of Sertoli-germ cell isolation,A 0.1％ DMSO solvent control group and three FLC exposure groups (10-6、10-7、104 mol/L) were selected,cultured cell for 24h,then MTr assay and index detection of oxidative stress were performed.Results The mortality of primary co-cultured sertoli-germ cell exposedto 10-6mol/LFLC was significantly higher than controlgroupandFLC exposure groups (10-7、10-8mol/L) (P＜0.05).10-6mol/L FLC reduced enzyme activity of CAT,SOD and GSH.Px,depressed GSH level,elevated MDA level,and had significant difference than control group and FLC exposure groups (10-7、10-8 mol/L) (P＜ 0.05).10-7 mol/L FLC decreased enzyme activity of CAT than control group,depressed enzyme activity of SOD than 10-8 mol/L FLC,lowered GSH level than control group and FLC exposure groups (10-8 mol/L),and had statistical difference (P＜0.05).Conclusion FLC can damage primary co-cultured sertoli-germ cell of rat,its possible mechanism is relevant to oxidative stress.

OBJECTIVETo investigate the effects of fluorochloridone (FLC) exposure on the testes of adult Sprague-Dawley (SD) rats.

METHODSForty male SD rats were randomly divided into four groups. These groups, each of 10 male rats, were separately given FLC by gavage at a dose of 0 (control), 30, 150, or 750 mg/kg once daily for 28 d. The oxidative stress biomarkers in the testes were measured by spectrophotometry. The pathological changes in testicular tissues were evaluated under the light and electric microscopes. The cauda epididymal sperm count was determined. The testicular toxicity of FLC was assessed accordingly.

RESULTSCompared with the control group, the 750 mg/kg FLC group had significantly lower testicular weight and organ coefficient, epididymal weight, and cauda epididymal sperm count (P < 0.05 or P < 0.01), the 150 and 750 mg/kg FLC groups had significantly increased malonaldehyde content (P < 0.05 or P < 0.01), each exposed group had a significantly reduced glutathione (GSH) level (P < 0.05 or P < 0.01), the 750 mg/kg FLC group had significantly reduced activities of superoxide dismutase (SOD), catalase (CAT), GSH peroxidase, GSH S-transferase (GSH-ST), and GSH reductase (GSH-GR) (P < 0.05 or P < 0.01), the 150 mg/kg FLC group showed significant decreases in the activities of all antioxidant enzymes except GSH-GR (P < 0.05 or P < 0.01), and the 30 mg/kg FLC group showed significant decreases in the activities of SOD and CAT (P < 0.05 or P < 0.01). Furthermore, seminiferous epithelial degeneration, Sertoli cell vacuolization, spermatogenic cell loss, and nuclear damage were observed under the light and electronic microscopes in the 150 and 750 mg/kg FLC groups.

CONCLUSIONFLC could damage the testes of adult rats by inducting oxidative stress. This research provided clues and directions for further exploration of the mechanism of FLC testicular toxicity.

Animals ; Male ; Oxidative Stress ; drug effects ; Pyrrolidinones ; toxicity ; Rats ; Rats, Sprague-Dawley ; Sperm Motility ; drug effects ; Testis ; drug effects ; metabolism ; pathology