Echocardiography plays an important role in diagnosing cardiac tumors and is helpful for subsequent treatment decision making.However,conventional echocardiography can hardly meet the clinical requirements of sufficient diagnosis,mostly due to the fact that it usually does little on identification of benign and malignant characters of tumors.Contrast echocardiography is a new technology using microbubbles as a contrast agent to trace the blood flow,which promotes the definition of endocardium lines and helps to reveal spaceoccupying abnormities in heart.Moreover,myocardial contrast echocardiography can be used to evaluate perfusions of intracardiac masses,and thus improves the accuracy of differential diagnosis enormously.

Objective To construct hns gene overexpression strains of Burkholderia thailandensis BPM,and explore the effects of hns overexpression on bacterial growth,motility,biofilm formation ability and susceptibility to antimicrobial drugs.Methods The hns genes 03253 and 05307 were respectively cloned into the expression vector pTAC-Cm to yield recombinant vectors pTAC-03253 and pTAC-05307.Then the 2 types of plasmids were electroporated into the BPM competent strain to obtain recombinant strains,and their phenotypic changes in growth,motility,biofilm,and drug sensitivity were detected.Results The strains of hns overexpression were successful constructed,and they presented significantly inhibited growth and motility,markedly enhanced ability to form biofilms,decreased sensitivity to levofloxacin and minocycline when compared with the wild-type BPM strain.Conclusion H-NS protein plays an important role in the regulation of virulence and drug resistance in Burkholderia thailandensis BPM,and can become a new target for the treatment of infections caused by Burkholderia thailandensis.

Objective To explore the effects of music relaxation imagination training and warm paste on hypothermia, stress response and shivering during cesarean section.Methods A total of 120 maternals who underwent cesarean section in Huai'an Maternal and Child Health-Care Center of Jiangsu Province from March 2016 to April 2017 were selected as the research objects. According to the different nursing method, the maternals were divided into observation group and control group, with 60 cases in each group. The maternals of the control group were given the conventional nursing intervention, on the basis of which the observation group was given music relaxation imagination training combined with warm paste. The axillary temperature, heart rate and the incidence of shivering of all research objects were statistically analyzed.Results The axillary temperature and heart rate in the observation group were stable during caesarean section. However, the decline of axillary temperature and the fluctuation of heart rate in the control group were obvious. The total incidence rate of shivering of the observation group was 6.67％, which was lower than that of the control group (21.67％), and the difference was statistically significant (χ2=5.74,P=0.03).Conclusions Music relaxation imagination training combined with warm paste can reduce hypothermia during cesarean section, and prevent stress response. In addition, it can effectively prevent intraoperative shivering and reduce the degree of shivering.

Objective:To investigate the clinical, imaging and genetic features of patients with global developmental delay combined with epilepsy and striatal degeneration caused by POLR3A gene mutations.Methods:A total of three patients from two families with non-consanguineous marriages admitted to the Department of Pediatric Neurology of Xiangya Hospital of Central South University in 2020 were examined in detail. Peripheral blood DNA was extracted, and whole-exome sequencing was performed on the patients, combined with Sanger sequencing for verification. The mutation and protein function predictor softwares were applied to analyze the mutation sites.Results:All three patients presented with global developmental delay, seizures, dystonia. Head magnetic resonance imaging of all patients suggested basal ganglia atrophy and striatal degeneration. All had compound heterozygous mutations of c.1980 G>C; c.1771-6 C>G and c.2044C>T; c.1771-7 C>G in POLR3A gene as indicated by whole-exome sequencing. Sanger sequencing validation confirmed that the compound heterozygous mutations were originated from the parents of probands from the two families, respectively. Bioinformatic analysis suggested pathogenic features of the mutations.Conclusions:Compound heterozygous mutations in POLR3A gene , including a splice site mutation result in global developmental delay combined with epilepsy, striatal degeneration. Clinicians should promote the awareness of POLR3 related spectrum disorders, thus make early recognition and diagnosis.

China prospective multicenter birth cohort (Prospective Omics Health Atlas birth cohort, POHA birth cohort) study was officially launched in 2022. This study, in collaboration with 12 participating units, aims to establish a high-quality, multidimensional cohort comprising 20 000 naturally conceived families and assisted reproductive families. The study involves long-term follow-up of parents and offspring, with corresponding biological samples collected at key time points. Through multi-omics testing and analysis, the study aims to conduct multi-omics big data research across the entire maternal and infant life cycle. The goal is to identify new biomarkers for maternal and infant diseases and provide scientific evidence for risk prediction related to maternal diseases and neonatal health.

Humans ; Cardiologists ; Neoplasms/drug therapy* ; Medical Oncology ; Heart ; Oncologists

Metabolic reprogramming is a hallmark of cancer, including lung cancer. However, the exact underlying mechanism and therapeutic potential are largely unknown. Here we report that protein arginine methyltransferase 6 (PRMT6) is highly expressed in lung cancer and is required for cell metabolism, tumorigenicity, and cisplatin response of lung cancer. PRMT6 regulated the oxidative pentose phosphate pathway (PPP) flux and glycolysis pathway in human lung cancer by increasing the activity of 6-phospho-gluconate dehydrogenase (6PGD) and α-enolase (ENO1). Furthermore, PRMT6 methylated R324 of 6PGD to enhancing its activity; while methylation at R9 and R372 of ENO1 promotes formation of active ENO1 dimers and 2-phosphoglycerate (2-PG) binding to ENO1, respectively. Lastly, targeting PRMT6 blocked the oxidative PPP flux, glycolysis pathway, and tumor growth, as well as enhanced the anti-tumor effects of cisplatin in lung cancer. Together, this study demonstrates that PRMT6 acts as a post-translational modification (PTM) regulator of glucose metabolism, which leads to the pathogenesis of lung cancer. It was proven that the PRMT6-6PGD/ENO1 regulatory axis is an important determinant of carcinogenesis and may become a promising cancer therapeutic strategy.