1.Effect of gamma globulin on serum immune index in neonatal pneumonia
Chinese Journal of Postgraduates of Medicine 2017;40(3):196-199
Objective To study the effect of gamma globulin on serum immune index in neonates with neonatal pneumonia. Methods One hundred neonates with neonatal pneumonia were selected, and they were divided into control group and observation group by random digits table method with 50 cases each. The control group was treated with conventional treatment, and the observation group was treated with conventional treatment combined with gamma globulin. The treatment efficacy, various signs disappearing times, length of stay in hospital and immune indexes were compared between 2 groups.Results The total effective rate in observation group was significantly higher than that in control group:96.00% (48/50) vs. 72.00% (36/50), and there was statistical difference (χ2 = 10.71, P<0.01). The disappearing times of cough, crackles, perioral cyanosis and fever and the length of stay in hospital in observation group were significantly shorter than those in control group:(4.39 ± 1.46) d vs. (6.32 ± 1.61) d, (4.21 ± 0.93) d vs. (5.36 ± 1.08) d, (0.83 ± 0.15) d vs. (1.16 ± 0.22) d, (2.53 ± 1.04) d vs. (4.47 ± 1.13) d and (7.68 ± 0.87) d vs. (10.03 ± 1.18) d, and there were statistical differences (P<0.01). The IgG, IgA, IgM, CD4+and CD4+/CD8+levels after treatment in observation group were significantly higher than those in control group: (10.32 ± 2.86) g/L vs. (6.88 ± 0.83) g/L, (1.14 ± 0.13) g/L vs. (0.91 ± 0.25) g/L, (0.94 ± 0.21) g/L vs. (0.85 ± 0.27) g/L, 0.516 ± 0.068 vs. 0.385 ± 0.063 and 1.89 ± 0.35 vs. 1.57 ± 0.33, but the CD8+was significantly lower than that in control group:0.303 ± 0.046 vs. 0.349 ± 0.048, and there were statistical differences (P<0.05). Conclusions The gamma globulin can not only significantly improve the curative effect in neonates with neonatal pneumonia and promote the recovery of the clinical symptoms, but also improve the efficiency of cell immunity and humoral immunity ability. It is a kind of effective treatment.
2.Progress in research on human thyroid cancer xenograft nude models
International Journal of Surgery 2015;42(8):551-554
Thyroid cancer is the most common malignant endocrine tumor.The xenograft nude mice model of thyroid cancer have played an important role in studying thyroid cancer.Different mouse model has distinctive characters and limitations,so it is important to choose a suitable mice model due to the experiment purpose.In this paper,the authors will address the research progress of human thyroid cancer xenograft nude models.
3.INDUCTION OF MYOCARDIUM CELLS FROM P19 CELLS USING DIMETHYL SULFOXIDE IN VITRO
Acta Anatomica Sinica 1954;0(02):-
Objective Observing the process of cell aggregation and differentiating into myocardium after the P19 cells treated with different methods and dimethyl sulfoxide(DMSO) inducer.Methods P19 cells were cultivated with DMSO in suspension for 7 days in Petri dishes or dishes containing a thin layer of soft agar to form cell aggregation.Then the aggregates were plated on gelatin-coated culture dishes,and cultured without DMSO to 19 days.The beating of cells was observed.?-sarcomeric actin and cardiac Troponin T(cTnT) immunocytochemical stains were used to identify cell differentiation.Results Cell aggregations were cohering-cultured with growth culture medium after DMSO induced for 7days,spontaneous and rhythmically beating cells were present within the aggregation outgrowths at day 15,which were ?-sarcomeric actin-positive and cTnT-positive.By day 19,positive rates were about 26% and 15% respectively.Conclusion By using DMSO exposure combination prior aggregation can induce P19 cells differentiate into cardiac myocytes which can spontaneously and rhythmically beat.
4.Imaging Analysis of Enlargement of Cervical Lymph Node
Journal of Practical Radiology 2001;0(10):-
Objective To study the imaging features of cervical lymphnode enlargement so as to improve the diagnosis of cervical lymph node disease.Methods CT and MRI appearances of 66 patients with pathologically-proved cervical lymph nodes disease were comparatively analyzed.Results There were significant difference in distribution of the multiple metastatic lymph node and lymph node tuberculosis(P=0.006).The ring-shaped enhancement were found in 20 cases with metastatic lymph nodes,19 of which were shown as thin-wall enhancement,its rate was 95%, and 5 ring-shaped enhancement of lymph node tuberculosis were shown as thick-wall enhancement.The former of which was significant difference than that of the latter(P=0.000).Of all central necrosis,it was significant difference between the metastatic lymph nodes(78%) and lymphoma(36%)(P=0.018).Conclusion According to the imaging features of cervical lymph nodes enlargement,their location and distribution,all of them may be diagnosed correctly.
7.Mitochondrial mechanisms of apoptosis induced by artesunate in human leukemia HL60 cells
Lei NIE ; Yilong YIN ; Shaofu YIN ;
Chinese Journal of Clinical Pharmacology and Therapeutics 2000;0(02):-
AIM: To explore mitochondrial mechanisms of apoptosis induced by artesunate in human leukemia HL60 cells. METHODS: Human leukemia HL60 cell lines were treated by artesunate. The inhibitory effect on cell proliferation was assessed by MTT assay. Electron microscopy and DNA gel electrophoresis was applied to demonstrate the presence of apoptosis. The level of MMP, cell cycle were determined by flow cytometry (FCM). The activity of caspase 3 was observed by colorimetry. RESULTS: Art showed a dose dependent and time dependent manner on the growth of HL60 cells (P
8.Effects of combined exercise program on the executive function in primary students with Chinese learning difficulties
Aiguo CHEN ; Lei CUI ; Hengchan YIN
Chinese Journal of Behavioral Medicine and Brain Science 2016;25(12):1123-1127
Objective To explore the effects of combined exercise program ( fun game + rope skip-ping + figure running) on executive function ( EF) in primary students with Chinese learning difficulties. Methods Fifty-four Chinese learning disabled students were selected and randomly divided into experimen-tal group and control group. Three tasks ( the Flanker task,the 1-back task and the More-odd shifting task) were used to measure pupil’ s EF ( inhibition,updating,shifting) . Results ( 1) Compared with the control group,the primary students with Chinese learning difficulties in experimental group showed a significant en-hancement in executive function (F(1,43)=9.45, P<0.01). (2) The positive effects of 10 weeks combined ex-ercise program on executive function in primary students with Chinese learning difficulties were different. Specifically,a beneficial effect on updating(F(1,43)= 11.37, P<0.01) was got,but there were no significant effects on inhibition (F(1,43)=1.52, P>0.05) and shifting(F(1,43)=2.57, P>0.05). Conclusion The effect of combined exercise program ( fun game+rope skipping+figure running) plays a positive role in promoting updating function of primary school students with Chinese learning difficulties.
9.Applied anatomy and cast observation in lingual arterial chemoembolization
Xuemin YIN ; Jingxing DAI ; Lei YU
Journal of Practical Stomatology 2000;0(06):-
Objective: To provide anatomical basis for the chemotherapy of lingual arterial embolization in clinic. Methods: The origin, course, branch distribution and anastomosis of the lingual artery were observed in 15 cases of adult head specimens. Results: As one of principal branches, the lingual artery arises from the external carotid artery at the level of the major cornu of hyoeides, approaches to the carotid bifurcation. With original diameter of (2.4?0.3) mm, it runs upward and passes deepwards to the hyoglossus muscle, gives off the dorsal lingual artery and the terminal branch -profunda lingual artery. Limited in each side of intrinsic muscles, two profunda lingual arteries creep tortuously along the muscular fibers and anastomose freely to structure submucous arterial rete. Conclusion: With concentrated origins, wide vascular diameter, constant course and enrichment of submucous arterial rete, the lingual artery is an ideal blood vessel for arterial chemoembolization.
10.The Effects of Different CO2 Pneumoperitoneum Pressures on Laparoscopic Cholecystectomy under Epidural Anesthesia: A Prospective Randomized Controlled Trial
Qinghua YIN ; Yajin CHEN ; Lei ZHANG
Chinese Journal of Minimally Invasive Surgery 2005;0(09):-
0.05).Conclusions Epidural anesthesia and lower pneumoperitoneum pressure(8 mm Hg) can completely meet with demands of most LC operations,and have the advantages of safety,economy and minimal invasion.