1.Expression of vascular endothelial growth factors (VEGF)-A, -C and -D and their prognostic significance and relationship with angio- and lymphangiogenesis in gastric cancer.
Xiao-lei WANG ; Zi-sheng AI ; Jian-ping FANG ; Ru-yong TANG ; Xi-mei CHEN
Chinese Journal of Oncology 2008;30(11):837-843
OBJECTIVETo investigate the expressions of vascular endothelial growth factors (VEGF)-A, -C and -D and their prognostic significance and relation to angio- and lymphangiogenesis in gastric cancer.
METHODSThe expression of VEGF-A, -C and -D in 123 primary gastric cancers was detected by immunohistochemical staining. The lymphatic vessel density (LVD) and microvessel density (MVD) were assessed after immunohistochemical double-staining with D2-40 and CD34, respectively. The correlation between the expression of those VEGF factors and clinicopathological parameters were analyzed by univariate method. The overall survival was evaluated by Kaplan-Meier method and log-rank test. Multivariate analysis was carried out using Cox proportion hazard model.
RESULTSThe positive expression rate of VEGF-A, -C and -D in primary gastric cancer samples were 64.2%, 65.9% and 41.5%, respectively. High expression of VEGF-A, or -C or -D, or any two of them was correlated with high LVD (P < 0.05). High expression of both VEGF-A and -C was associated with high MVD, lymph node metastasis, LVI and MVI (P < 0.05). Both VEGF-C and -D high expression was correlated with LVI and lymph node metastasis (P < 0.05). The patients with high expression of these factors had a statistically shorter overall survival (P < 0.05). The patients with both VEGF-A and -C expression had the shortest survival (56 months). Multivariate analysis showed that VEGF-A high expression, MVD, lymph node metastasis and depth of tumor invasion were independent survival predictors (P = 0.033, 0.002, 0.019 and P < 0.001, respectively).
CONCLUSIONHigh expression of both VEGF-A and -C imply high potential of lymphangiogenesis, metastasis and poorer survival in gastric cancer patients. High expression of VEGF-C and -D may induce lymphangiogenesis and promote lymph node metastasis, but only VEGF-A is an independent predictor of survival.
Adult ; Aged ; Aged, 80 and over ; Female ; Follow-Up Studies ; Humans ; Lymphangiogenesis ; Lymphatic Metastasis ; Lymphatic Vessels ; pathology ; Male ; Microvessels ; pathology ; Middle Aged ; Neovascularization, Pathologic ; Proportional Hazards Models ; Stomach Neoplasms ; metabolism ; pathology ; Survival Rate ; Vascular Endothelial Growth Factor A ; metabolism ; Vascular Endothelial Growth Factor C ; metabolism ; Vascular Endothelial Growth Factor D ; metabolism
2.Relationship between serum homocysteine and metabolic syndrome: a cross-sectional study
Sheng-Qiang FENG ; Ping YE ; Lei-Ming LUO ; Wen-Kai XIAO ; Ru-Yi XU ; Hong-Mei WU
Chinese Journal of Epidemiology 2012;33(3):256-259
Objective To explore the relationship between serum homocysteine and metabolic syndrome (MS).Methods A cohort with 1680 people involved in a community-based population in Beijing was investigated.Metabolic syndrome was defined by NCEP-ATP Ⅲ criteria.Multivariate logistic regression analysis was used to estimate the odds ratios (OR) of MS.Multiple linear regression analysis was performed to analyze the association between Hcy and characteristic variables.Results Homocysteine was higher in MS population compared to those without MS ( 17.99 μmol/L vs.17.18 μmol/L,P=0.007) after adjusted for age and sex.Levels of homocysteine increased with the presence of MS components (from 0 to 4 or 5) (16.71,16.94,17.62,18.20,17.82 μmol/L respectively,P=0.044 for linear trend).Among the components,groups with larger waist circumference,higher blood pressure and triglycerides showed significantly higher Hcy level than their counterparts.Results from multiple logistic regression analysis revealed that the highest Hcy quartile (Hcy Ⅳ ) was significantly associated with MS.Compared with the lowest Hcy quartile (Hcy Ⅰ ),the adjusted odds ratio of having MS in HcyⅣ was 1.379(1.005-1.892) after adjusting for age,sex,levels on creatinine/estimated glomerular filtration rate (eGFR)/low-density lipoprotein cholesterol (LDL-C) and uric acid,smoking,alcohol intake and exercise.In the partial correlation analyses,Hcy was positively associated with body mass index (BMI),waist circumsternece,blood pressure,LDL-C,triglycerides (TG),uric acid,serum creatinine,eGFR,but inversely associated with high-density lipoprotein cholesterol (HDL-C) and independently with age and sex.In multiple linear regression analysis,age,male sex,BMI,LDL-C,creatinine and uric acid were found to be independently associated with Hcy level.Conclusion There was an association noticed between the MS using NCEP-ATP Ⅲ criteria and the highest quartile level of Hcy in this study.Factors as age and being male,the levels of BMI,LDL-C,creatinine and uric acid were independently associated with the Hcy level.
3.Relationship between serum N-terminal pro-brain natriuretic peptide and metabolic syndrome: a cross-sectional study
Sheng-Qiang FENG ; Ping YE ; Lei-Ming LUO ; Wen-Kai XIAO ; Ru-Yi XU ; Hong-Mei WU
Chinese Journal of Cardiology 2013;41(2):130-134
Objective To explore the relationship between serum N-terminal pro-brain natriuretic peptide (NT-proBNP) and metabolic syndrome (MS).Methods A total of 1323 Beijing residents (559male) were investigated.MS was defined by the modified 2004 Chinese Diabetes Society criteria and 439 cases were diagnosed as MS according to this criteria.Multivariate logistic regression analysis was used to estimate the odds ratios (OR) of MS.Multiple linear regression analysis was performed to analyze the association between NT-proBNP and characteristic variables.Results NT-proBNP was significantly lower in MS group compared to non-MS group [32.51 (29.17,36.14) ng/L vs.38.55(35.73,41.50) ng/L,P =0.012] after adjusted for age and gender.NT-proBNP level decreased with the presence of MS components(from 0 to 4 or 5) (45.92,37.24,35.40,31.55 and 33.65 ng/L respectively,P =0.043 for linear trend).Among the components,groups with larger waist circumference,higher fasting glucose and triglycerides were associated with lower NT-proBNP level.After adjustment for potential confounders,compared with the lowest NT-proBNP quartile,the adjusted odds ratio of the second,third and fourth quartile for having MS were 0.782 (95 % CI: 0.544-1.122,P > 0.05),0.709 (95 % CI: 0.489-1.028,P > 0.05),0.604 (95 % CI:O.405-0.900,P <0.05),respectively.Multiple linear regression analysis showed that female gender(β =0.248,P <0.001),age(β =0.167,P < 0.001),systolic blood pressure (β =0.154,P < 0.001) were positively related to NT-proBNP level while waist circumference (β =-0.082,P =0.004),diastolic blood pressure (β =-0.085,P =0.015),triglycerides (β =-0.101,P < O.001),total cholesterol (β =-0.078,P =0.004),eGFR (β =-0.150,P < 0.001) were negatively corelated to NT-proBNP level.Conclusion In this cohort,higher serum NT-proBNP concentration is associated with lower incidence of metabolic syndrome.
4.Association between peroxisome proliferator-activated receptor gene single nucleotide polymorphisms and arterial stiffness in adult Chinese population
Ru-Yi XU ; Ping YE ; Li SHENG ; Lei-Ming LUO ; Hong-Mei WU ; Wen-Kai XIAO
Chinese Journal of Cardiology 2013;41(4):288-292
Objective To analyze the association between single nucleotide polymorphisms(SNPs)of peroxisome proliferator-activated receptor(PPAR) and arterial stiffness in adult Chinese population (> 50 years).Methods Cardiovascular risk factors from participants of Beijing epidemiological investigation were analyzed.Carotid-femoral pulse wave velocity (cfPWV) was measured by Complior system.The subjects were divided into normal arterial stiffness group (cfPWV < 12 m/s,n =844) and increased arterial stiffness group (cfPWV > 12 m/s,n =530).Three valid SNPs including rs1053049,rs1800234 and rs8192678 in the PPAR and PPARγCla gene were genotyped by TaqMan allelic discrimination assays.Results The age [(67.9 ±8.8) years vs.(58.0 ±9.7) years],prevalence of hypertension[71.1% (377/530) vs.30.5% (257/844)] and diabetes mellitus [21.7% (115/530) vs.11.0% (93/844)] were all significantly higher in increased arterial stiffness group than in normal group (all P < 0.05).The frequencies of CC,CT and TT type of rs8192678 [CC:32.2% (272/844) vs.30.8% (163/530),CT:48.7% (411/844) vs.52.1% (276/530),TT:19.1% (161/844) vs.17.2% (91/530)],rs1053049 [CC:55.7% (470/844) vs.51.3% (272/530),CT:36.7% (310/844) vs.39.1% (207/530),TT:7.6% (64/844) vs.9.6% (51/530)] and rs1800234 [CC:88.4% (746/844) vs.90.4% (479/530),CT + TT:11.6% (98/844) vs.9.6% (51/530)] were similar between the two groups.There was also no association between haplotypes and the increased arterial stiffness in this cohort.Conclusions In this community-based population,we found that aging,hypertension and diabetes mellitus were associated but SNPs of PPAR and PPARγCla were not associated with arterial stiffness.
6.Effects of bradykinin on the proliferation, apoptosis and differentiation of human keratinocytes.
Li-wei RAN ; Wei-ming TAN ; Sheng-shun TAN ; Ru ZHANG ; Zhen-ping CAO ; Xiao-Bing LEI
Chinese Journal of Burns 2005;21(4):289-292
OBJECTIVETo investigate the effects of bradykinin (BK) on the proliferation, apoptosis and differentiation of human keratinocyte (HKC) and the underlying mechanisms.
METHODSHKCs were cultured together with 1 x 10(-4) - 1 x 10(-9) mol/L of BK. With methyl thiotetrazole (MTT) and trypan blue staining it was shown that the BK in dose of 1 x 10(-4) mol/L possessed most powerful inhibitory effect, and the survival rate of HKC was 69.3%. Therefore, BK was employed in the dose of 1 x 10(-4) mol/L in the following studies. When the growth of HKCs reached the logarithmic phase, BK in the concentration of 1 x 10(-4) mol/L was added, and it was categorized as the test group (E). HKCs without BK served as the control group (C). The cell cycle and apoptosis were detected by flow cytometry after being cultured for 24 and 48 hours. The change in intracellular calcium [Ca(2+)](i) was determined by means of laser scanning confocal microscopy with calcium fluorescence probe Fluo-3/AM technique. The expression of HKC differentiation labeling protein keratin10 (K10) and involucrin were detected with Strept Avidin-Biotin Complex (SABC) immunocytochemical assay.
RESULTSThe cell ratio in G0/G1 phase in E group increased by 34.57% while in S phase decreased by 58.91% in reference to that in C group. The G1/S phase switching of HKCs was obviously inhibited by BK, and apoptosis was stimulated (apoptotic rate of 15.34% in E group vs 5.60% in C group, P < 0.05). The [Ca(2+)](i) increased transiently in HKCs by 163.0% in E group after 3 minutes of BK activation and decreased thereafter in reference to that in C group. The K10 expression in HKC was down-regulated in E group with positive cell rate of 2.20%, which was lower than that of C group (6.89%, P < 0.05).
CONCLUSIONThe cell cycle process of HKC could be inhibited by high concentration of BK with increased apoptosis and an increase in [Ca(2+)](i), which might be the mechanism of inhibition of growth of HKC in vitro. Furthermore, the epithelial regeneration and HKC differentiation can also be inhibited by BK.
Apoptosis ; drug effects ; Bradykinin ; pharmacology ; Cell Cycle ; Cell Differentiation ; drug effects ; Cell Proliferation ; drug effects ; Cells, Cultured ; Humans ; Keratinocytes ; cytology ; metabolism ; Keratins ; metabolism
7.Study on the source of human infection with H9N2 influenza virus from live poultry markets in Changsha
Ru-sheng ZHUANG ; Zheng HUANG ; Xiao-lei LIU ; Dong YAO ; Jing-fang CHEN ; Jie YUAN ; Xin-hua OU ; Bian-cheng SUN
Chinese Journal of Disease Control & Prevention 2019;23(5):567-572
Objective To explore the source of human infection H9N2 avian influenza virus (AIV). Methods Environmental AIV nucleic acid monitoring was conducted for live poultry markets in Changsha city from 2014 to 2015, and data of human infection H9N2 subtype AIV cases worldwide were collected. Phylogenetic trees of hemagglutinin(HA), neuraminidase(NA)and non-structural protein(NS)genes from human infection H9N2 subtype AIV, the live poultry markets environmental H9N2 subtype AIV and partial avian H9N2 subtype AIV were constructed using the MEGA 6.06 software, respectively. Results In 2014-2015, H9 subtype AIV had the highest nucleic acid positive rate (44.76%) in the live poultry markets environment of Changsha city, and the pollution was serious. A total of 27 cases of human infection with H9N2 subtype AIV had been reported worldwide, and most of these patients recovered after treatments.Epidemiological survey showed that 59.26% (16/27) of cases had a clear history of exposure to poultry or live poultry markets. The phylogenetic trees of HA, NA and NS genes showed that the human infection H9N2 subtype AIV isolates isolated from Hunan and Guangdong were closely related to the H9N2 subtype AIV isolated from the live poultry markets environment in Hunan and Guangdong provinces from 2013 to 2016. The nucleotide similarity was as high as 97%-99%. Conclusion Live poultry market is one of the sources of human infection with H9N2 influenza virus.
8.Correlation between obesity index and blood pressure in Kazak childhood from Yili, Xinjiang
Yong-Jie XU ; Min LI ; Pei-Ru XU ; Ying-Sheng ZHENG ; Lei ZHANG ; Ming-Gang YE ; Yu LIU
Chinese Journal of Epidemiology 2012;33(8):774-778
Objective To investigate the correlations between obesity index such as waist circumference,hip circumference,skin-fold thickness,body mass index,waist to hip ratio,waist to height ratio etc,and blood pressure among Kazak children living in Yili of Xinjiang.Methods In May-June 2009,2438 Kazak children aged 7 to 14 at primary schools in Yili of Xinjiang,were investigated.Waist circumference,hip circumference,skin-fold thickness,systolic and diastolic blood pressure were measured to calculate the body mass index,waist to hip ratio and waist to height ratio.Analysis on the differences in age and gender,blood pressure (BP) between hypertensive or normal BP group under the standard set for children.SPSS 17.0 software was used for statistical analysis on its correlation.Results The total prevalence of hypertension was 5.7% in Kazak ethnic school-age children in Yili,with 4.4% in the boys and 7.0% in girls.Data from waist circumference,hip circumference,waist-height-ratio,waist-hip ratio,systolic and diastolic blood pressure showed gender and age differences (P=0.000).Average systolic blood pressure was correlated to waist circumference,hip circumference,skin-fold thickness,body mass index and waist to hip ratio (P=0.000),with hip circumference the most (man:r=0.618,female:r=0.655).The average diastolic blood pressure was correlated to waist circumference,hip circumference,skin-fold thickness,body mass index and waist to hip ratio (P=0.000),with hip having the strongest relation (r=0.489 for male and r=0.548 for females).Significant differences were seen between Hypertension groupand normal blood pressure group on waist circumference,hip circumference,skin-fold thickness,body mass index and waist-hip ratio (P<0.05).Conclusion In Yili of Xinjiang,the prevalence of hypertension was at the low level in Kazak ethnic school-aged children when comparing to data from national and foreign standards.Indicators related to obesity as waist circumference,hip circumference,body mass index and waist to hip ratio were positively correlated with blood pressure,with hip in particular.
9.A 26-year clinical observation of splenic auto-transplantation and oesophageal transection anastomosis: a new treatment strategy in patients with portal hypertension.
Lei ZHANG ; Jin-shan HUO ; Hong-wei ZHANG ; Ru-fu CHEN ; Jie ZHANG ; Obetien MAPUDENGO ; Tian-lin FANG ; Ya-jin CHEN ; Qing-jia OU ; Ji-sheng CHEN
Chinese Medical Journal 2007;120(6):452-457
BACKGROUNDSurgical treatment options for patients with cirrhosis and portal hypertension are complicated. In this study, we evaluated the effectiveness of a new treatment strategy, splenic auto-transplantation and oesophageal transection anastomosis. We report results from clinical observations, splenic immune function and portal dynamics in 274 patients.
METHODSFrom 1979 to 2005, 274 cirrhosis patients with portal hypertension underwent the new treatment strategy, and were followed up to compare results with those patients who underwent traditional surgical treatment. From 1999 to 2002, a randomized controlled trial (RCT) was performed on 40 patients to compare their post-operative immune function. From 1994 to 2006, another RCT enrolled 28 patients to compare portal dynamics using three-dimensional dynamic contrast-enhanced magnetic resonance angiography (3D DEC MRA) investigation post operation.
RESULTSAmong 274 patients (mean age 41.8 years), the emergency operative mortality (4.4%), selective operative mortality (2.2%), complication rate (17.9%), prevalence of hepatic encephalopathy (< 1%), rate of portal hypertension gastritis (PHG) bleeding (9.1%), and morbidity of hepatic carcinoma (8%) were similar to those patients undergoing traditional operation; the spleen immunology function (Tuftsin, IgM) decreased in both groups 2 months post operation, but this decrease did not reach statistical significance. Through 3D DCE MRA, the cross sectional area and the velocity and volume of blood flow of the main portal vein decreased significantly after operation in both groups. The velocity and volume of blood flow in the auto-transplantation group was significantly lower than that in the control group.
CONCLUSIONSSplenic auto-transplantation and esophageal transection anastomosis is a safe, effective, and reasonable treatment strategy for patients with portal hypertension with varicial bleeding. It not only can correct hypersplenism, but may also achieve complete hemostasis. Spleens auto-transplanted into the retroperitoneal space can preserve immune function and establish broad collateral circulation.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Anastomosis, Surgical ; Child ; Esophagus ; surgery ; Female ; Humans ; Hypertension, Portal ; immunology ; surgery ; Imaging, Three-Dimensional ; Immunoglobulin M ; blood ; Male ; Middle Aged ; Prospective Studies ; Spleen ; transplantation ; Transplantation, Autologous
10.Associations of plasma homocysteine and high-sensitivity C-reactive protein levels with arterial stiffness in Chinese population: a community-based study.
Sheng-Qiang FENG ; Ping YE ; Lei-Ming LUO ; Wen-Kai XIAO ; Yong-Yi BAI ; Dan FENG ; De-Jun LIU ; Hong-Mei WU ; Ru-Yi XU ; Jie BAI
Chinese Medical Journal 2012;125(1):44-49
BACKGROUNDArterial stiffness increases with age and is also associated with traditional cardiovascular risk factors. Little is known about the relations of homocysteine and high-sensitivity C-reactive protein (hs-CRP) to arterial stiffness in the Chinese community. The aim of the present study was to investigate the association of plasma homocysteine and hs-CRP levels with arterial stiffness in a community-based cohort.
METHODSWe related levels of homocysteine and hs-CRP to four measures of arterial stiffness (carotid-femoral pulse wave velocity (PWV), carotid-radial PWV, carotid-ankle PWV and heart rate corrected augmentation index) in 1680 participants from two communities of Beijing, China. Arterial stiffness was measured within two days of the time of biomarker measurement.
RESULTSIn univariate analysis, homocysteine was positively associated with the carotid-femoral PWV (r = 0.211, P < 0.0001), carotid-radial PWV (r = 0.120, P < 0.0001) and carotid-ankle PWV (r = 0.148, P < 0.0001), whereas it was inversely related to the augmentation index (r = -0.052, P = 0.016). Hs-CRP was positively associated with the carotid-femoral PWV (r = 0.074, P = 0.001) and carotid-ankle PWV (r = 0.050, P = 0.02). In multiple-adjusted models (R(2) = 0.57), homocysteine levels remained a significant determinant of the carotid-femoral PWV (standardized β = 0.065, P = 0.007), whereas the association of hs-CRP with measurements of arterial stiffness was not present.
CONCLUSIONSIn the Chinese population, plasma homocysteine levels are associated with alterations of aortic stiffness, whereas plasma levels of hs-CRP are not independently related to artery stiffening.
Aged ; Asian Continental Ancestry Group ; C-Reactive Protein ; metabolism ; China ; epidemiology ; Cross-Sectional Studies ; Female ; Homocysteine ; blood ; Humans ; Male ; Middle Aged ; Vascular Stiffness ; physiology