Objective To establish a simple,rapid,accurate and sensitive method for determination of free toluene diisocyanate in paint.Method The samples were diluted by acetone and separated using GC,25%SE-30column.The contents of free toluene diisocyanate in the samples of paint were analysed by gas chromatography.The qualitative and the quantitative analyses were carried out based on the retention time and peak height or peak area respectively.Re -sults The linear range of the method was0.000～0.025%with r=0.9997.The RSD was in the range of0.08～0.75%and the recovery rates ranged95.0～106.7%.Conclusion The method was simple,rapid and accurate,which was suit-able for the determination of free toluene diisocyanate in paint.

BACKGROUND: The root canal filling materials available have different degrees of noxious stimulation to periapical tissue and cannot induce growth and regeneration of bone at the periapical region. Moreover, the operation of canal filling is not easily controlled.OBJECTIVE: To investigate biocompatibility and osteoconductivity between periapical tissue and compound coral paste after root canal filling.DESIGN: Controlled observation.SETTING: Department of Stomatology in the Renmin Hospital of Wuhan University.MATERIALS: The experiment was carried out in the Key Laboratory for Oral Biomedical Engineering by the State Ministry of Education, School of Stomatology in Wuhan University from September 2002 to January 2006. Eight adult female mongrel dogs, weighed 15 kg, were offered by the Wuhan General Hospital of Guangzhou Military Area Command of Chinese PLA (SYXK-2003-0007). Each dog contained 32 root canals, and totally 256 root canals were utilized in this study.METHODS: Experimental animals underwent general anesthesia by the intravenous injection of sodium pentobarbital (30 mg/kg), then tracheal intubation was utilized for the root canal filling, and the canals were randomly distributed into two groups: experimental group (n=128) with compound coral paste (coral 40 g and iodoform 8 g, offered by the Key Laboratory for Oral Biomedical Engineering by the State Ministry of Education, School of Stomatology in Wuhan University); control group (n=128) with gutta-percha point+zinc oxide-eugenol sealer. One animal was sacrificed by anesthetic overdose at 2, 4, 12 and 24 weeks after root canal filling, dental film by X-ray and light microscope were used for the examination.MAIN OUTCOME MEASURES: ①Periapical inflammation: Cells were counted in a high power field, and three levels were defined as mild (＜100 cells/mm2), moderate (100-200 cells/mm2) and severe (＞200 cells/mm2).②destruction and regeneration of periapical tissues.③bone substitute of compound coral paste in periapical regions.RESULTS: Eight mongrel dogs were all involved in the result analysis.①Periapical shadow by X-ray film: Distinct shadow at root apex area can not be observed in the experimental group at each stage. While two cases appeared the root apex shadow in the control group at 12 and 24 weeks, respectively.②Histopathological observation: At 2 weeks after root filling,inflammatory cell infiltration was observed in each group, which was dominantly neutrocyte. There was a mild inflammation in experimental group and a moderate inflammation in control group. Four weeks after root filling, there were focal inflammatory cells infiltrated around the coral particles in the experimental group, but in the control group there were a great deal of inflammatory cells in periapical tissue. Twelve weeks after root filling, in experimental group, there was no inflammatory cell infiltration was observed, the deposition of bone combined tightly with coral particles was detected, and apical foramen became smaller;, but in the control group, there were still inflammatory cells circumvohiting the gutta-percha point. After 24 weeks, coral particles was not observed at the root filling region in the experimental group, and they were replaced by a great deal deposition of bones, root foramens were sealed completely and grew into root canal wall. Root apex was coated with fibrous tissue in the control group.CONCLUSION: This compound coral paste shows good compatibility after filling, promotes the osteoconductivity, and seals the root foremen, so it can be used as a root canal filling material.

Angioplasty, Balloon, Coronary ; Coronary Disease ; therapy ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Phytotherapy

ObjectiveTo evaluate and compare long-term clinical outcomes of arthroscopic anterior cruciate ligament (ACL) reconstruction with remnant preservation versus remnant resection.MethodsFrom October 1999 to May 2005,standard ACL reconstruction with autogenous quadrupled hamstring tendons under arthroscopy using remnant resection with transtibial techniques was conducted in 87 patients.From June 2005 to May 2010,ACL reconstruction with autogenous quadrupled hamstring tendons using outside-in bone tunnel establishment and remnant preservation was performed in 221 patients.Only the patients who underwent simple ACL reconstruction together with more than three years follow-up records were enrolled in the study,including 66 patients in remnant preservation group and 39in remnant resection group.ResultsPatients of both groups were all followed up for 36-60 months,which showed insignificant difference between groups ( P ＞ 0.05).Compared with the preoperative data,the latest follow up data demonstrated satisfactory outcomes in both groups.The two groups showed no significant differences regarding the preoperative data including age and gender distribution,average time from injury to surgery,joint instability extent and functional score of the affected knee ( P ＞ 0.05 ).There was no statistical difference in regards of the thigh muscle atrophy and Lachman test between groups ( P ＞0.05).However,the remnant preservation group was superior to the remnant resection group in aspects of the range of motion of the affected knee,Lysholm score,stability in anterior drawer test,stability in pivot shift test and international knee documentation committee (IKDC) grade ( all P ＜ 0.05 ). ConclusionCompared with standard ACL reconstruction using remnant resection with transtibial techniques,the ACL reconstruction using outside-in bone tunnel establishment and remnant preservation has better longterm clinical outcomes including stability and function of the affected knee.

Objective To evaluate the effective treatment for and prevention of complications. Methods The diagnosis and treatment of 32 patients with acute arterial ischemia of the lower extremity were analyzed (retrospectively). 4 cases were treated with thrombolytic therapy, 28 cases underwent emergency operation, of which 3 cases underwent amputation after embolectomy, and 25 cases were treated by arteriotomy of femoral artery and embolectomy with Fogarty catheter(7 cases underwent concomitant arterioplasty).Results One (patient) in the nonoperative group died; but there was no mortality in the operated group. Limb amputation was done in 3 cases, and limbs were saved in 28 cases. Conclusions For acute arterial ischemia of the lower (extremity), emergency arteriotomy of femoral artery plus embolectomy is the treatment of choice. This (therapeutic) approach can lower mortality and limb amputation rate.

China ; Critical Pathways ; Humans ; Medicine, Chinese Traditional ; Myocardial Infarction ; therapy

[Objective] To investigate the treatment in the use of allograft bone grains combined with autograft bone grafting in giant cell tumor.[Method]From March 1996 to March 2006,24 cases of giant cell tumor closed to knee were verified by pathology in this department,including 11 on thighbone and others on tibia.Before operation allografts was sheared to grains after antigen extracted by repeat freeze thawing and mechanical methods.Giant cell tumor of bone was completely erased in surgical fringe and the defect was thoroughly closed-up with allograft grains combined with cancellous bone autografts.The knee joints exercise was made for 12 weeks with external orthosis fixation.[Result]No severe complication occurred in early stage after operation in the 24 patients.All of them were followed up for 1 to 6 years with an average of 2 years and 8 months.None of them recidivated after the operation,and no insert was taken off for severe rejection,one case showed tolerant pathologic fracture.All the transplantations showed ideal appearance in X-ray photograph and the motion of joints had no significant difference than before.Curative effects were evaluated by Mankin criteria.The outcome was excellent in 20 cases,good in 4 cases.[Conclusion]Bone allograft grains combined with autograft is an effective and few rejection in filling of giant bone defect in surgical operation of giant cell tumor near knee joints.

[Objective]To explore the incidence, causes, training courses at high risk and location of the body of training injury in a special force corps, provide evidence for effective prevention of the injury.[Method]A questionnaire on training injuries and relative situations, and review of medical records from Nov 2005 to Oct 2006 were performed in 823 soldiers and officers in a special force corps at Nov 2006. Data were input into computers after quantization, and descriptive analysis was conducted with SPSS 13.0 software. [Result]Of 823 soldiers and officers, 738 (89.67%) experienced one or more injuries in that year, 525 (63.79%) were affected in normal training, 221 (26.85%) lost more than one training day due to the injuries. Confirmed with the medical records, 213 (25.90%) lost more than one training day due to injuries. A total of 1696 injuries occurred in 823 soldiers and offices in that year, of them, 1263 injuries interfered with normal training. Injury rate was 12.78 per 100 per month. 24.12% of injuries were due to over-exertion, 15.86% due to accident and 13.33% due to environmental field. Injury occurred mainly in runing related training courses including 5km running (22.17%) and 400m obstacle running (20.87%), followed by weight lifting (9.67%). The most frequent site of injury was the lower extremities, accounted for 65.74 % of the inj urie s including the foot ( 18.75 %),the ankle (13.50%), the leg (13.56%) and the knee (14.39%), followed by low back (14.21%). [Conclusion]Persistent over-exertion basic training, the courses at high risk of injury, should be improved in focus. Lower extremities and low back, the most frequent locations of injury, should be taken as key points of prevention and treatment of training injury.

OBJECTIVE:To investigate osteogenic and chondrogenic differentiation of adipose-derived stem cells following a short time induction with bone morphogenetic protein 2(BMP-2) or BMP-7.METHODS:The subcutaneous adipose tissue was obtained from adult New Zealand rabbits under aseptic condition,and cultured in vitro with collagenase digestion.All cells were divided into 3 groups:in the BMP-2 group,cells were cultured with medium containing 0.1 g/L vitamin C,10 mmol/L?-sodium glycerophosphate and 10?g/L BMP-2 for 10 minutes,followed by 4-14 days inoculation with density of 18?104 cells per pore.In the BMP-7 group,cells were cultured with BMP-7 with the same methods as BMP-2 group.The cells were cultured with simple culture medium in the control group.RESULTS:Compared to the control group,the number of adipose-derived stem cells,protein level and ALalkaline phosphatase(ALP) activity of BMP-2 and BMP-7 groups was up-regulated 1.78-1.79,1.15-1.95,and 32-40 times,respectively.At days 4 after a 15 minutes induction,the runx-2 gene expression,osteopontin gene,and biglycan gene were increased 1.9,2.2 and 1.3-1.7 folds than that of the control group.Meantime,only the biglycan gene expression was increased 1.3-1.7 folds in the BMP-7 group,the runx-2 gene expression,osteopontin gene was not changed.At day 14 after a 15 minutes induction,there was no alter of runx-2,osteopontin,biglycan,as well as aggrecan gene expression in the BMP-2 group;while down-regulated runx-2,osteopontin,biglycan 1.8,5.0 and 1.7 folds,with increased aggrecan gene in the BMP-7 group.CONCLUSION:Following a short time induction,BMP-2 can stimulate runx-2 and osteogenic expression at 4 days after re-culture,whereas BMP-7 down-regulate genes expression at days 14,yet down-regulate aggrecan mRNA expression.

ObjectiveTo observe the effect of early rehabilitation and insulin treatment on acute cerebral infarction. Methods160 cases with acute cerebral infarction (without diabetes and the serum glucose were all above 6.7 mmol/L) were divided into treatment group and control group. Each case of both groups was treated with routine treatment, and those in the treatment group were treated with insulin instantly and early rehabilitation in the first day after onset when physical signs were stable and conditions didn't deteriorate. They were assessed with Neurological Impairment, Fugl-Meyer Assessment and Barthel Index 4 weeks after treatment. ResultsCompared with the control group, the neurological function improved obviously in the treated group (P＜0.05). ConclusionEarly rehabilitation and insulin treatment can improve neurological function and the activity of daily living.