Objective To observe the efficacy of bioelectric stimulation of the pelvic floor combined with acupuncture in treating detrusor urine weakness after transurethral resection of the prostate (TURP) in patients with prostate hyperplasia.Methods Forty-seven cases with detrusor urine weakness after TURP were randomly divided into an experimental group and a control group.All of the patients received routine bladder function training and medication.In addition,patients in the experimental group were treated with bioelectric stimulation of the pelvic floor and with acupuncture.The differences between the two groups were compared using the International Prostate Symptom Score (IPSS),maximum urine flow rate (MFR),average flow rate (AFR),detrusor pressure at peak flow (PdetQmax) and residual urine volume in the bladder.Results No significant difference was found between the two groups before treatment,but at two weeks after treatment MFR,AFR and PdetQmax were significantly higher in the experimental group than in the control group,and residual urine volume and IPSS were significantly lower.Conclusion Bioelectric stimulation of the pelvic floor combined with acupuncture is more effective than routine bladder function training and medication alone for alleviating detrusor urine weakness after TURP.

Objective To analyze the application value of central region cervical lymph nodes dissection in papillary thyroid microcarcinoma (PTMC) with clinically node-negative sides of neck stage.Methods Clinical data of 72 PTMC patients with clinically node-negative sides of neck stage were collected,and they were underwent total thyroidectomy,isthmic portion excision and contralateral lobus glandularis excision and neck dissection.A long term follow-up was made.Results Twenty-four patients were found central region cervical lymph nodes metastasis while other 48 patients were not found central region cervical lymph nodes metastasis.Followed up for 1.5-4.5 (3.4 ± 1.1) years,4 patients were found recurrent laryngeal nerve paralysis and recovered within 4 months.Four patients were found central region cervical lymph nodes metastasis after operation the 3rd years.Conclusions There is a tendency for PTMC patients with clinically node-negative sides of neck stage to occur central region cervical lymph nodes metastasis,it's necessary to perform prophylactic central region dissection.It can decrease the complications and improve the prognosis.

National standards on food contact materials are an important part of national food safety standards in China.New standards system on food contact materials in China has been established through food standards clean-up,integration and other works.In this paper,the framework of the new standard system on food contact materials is summarized.The major changes of the new standard system are compared to the old one.The challenges confronted by the industries and the problems in the new standard system are scientifically analyzed.The suggestions for future work are proposed.

Carcinoma ; classification ; pathology ; Consensus ; Humans ; Thymoma ; classification ; pathology ; Thymus Neoplasms ; classification ; pathology ; World Health Organization

Objective To investigate the distribution and drug resistance state of Pseudomonas aeruginosa, so as to offer reasonable experimental data for clinical therapy. Methods A statistic analy- sis for the specimen souree, distribution of infected departments and drug resistance state to 441 iso- lates was conducted in a retrospective way. Results Among 441 strains, 357 (80. 95%) were isolated from sputum species. The department with the highest isolating rate was ICU (26.5%), followed by department of respiratory disease (20. 63 %), department of neurosurgery (19.05 %) and department of geriatrics (17. 01%). The most sensitive antibiotic was Meropenem, which total drug resistance rate was 13. 61%; the most insensitive antibiotic was ampicillin-sulbactam, which total drug resistance rate was 91.38%. The drug resistance rate of P. Aeruginosa was increasing year by year. Conclusion Clinical isolates of P. Aeruginosa mostly come from sputum samples and ICU, and they are highly and multidrug resistant to various antibiotics.

Objective_To identify the anti-HBV efficiency of AAV-IFNαby infecting HepG2.2.15 cell line, and to develop a new strategy of curing hepatitis B.Methods_Firstly,recombinant vector was packaged into AAV by three plasmids co-transfection method;then AAV-IFNαinfected HepG2.2.15 cell line, and samples were collected on the 1st, 3rd,6th and 9th day;thirdly, The time-course of HBV DNA,HBsAg and HBeAg were detected by qPCR and ELISA.The proliferation of HepG2.2.15 cells detected by MTT.Results_Recombinant AAV-IFNαcan significantly inhibit HBV replication and expression in vitro because of the proliferation of HepG2.2.15 cells inhibited by AAV6-IFNα( P<0.05) .Conclusions_AAV-IFNαis a potential antiviral product for hepatitis B.

OBJECTIVE To study the prevalence,pathogenic characteristics and drug resistance of the invasive fungal infection to provide the reference for clinical treatment.METHODS The fungi were cultured and identified conventionally.The susceptibility test was performed by K-B disk diffusion methods.The data were analyzed by WHONET 5.3 software.RESULTS Among 232 strains of fungi,isolating rate of fungi from sputum was the highest,accounting for 74.1%.The following was urine,accounting for 17.2%.The most common organisms were Candida(96.6%)among which white Candida albicans accounted for 58.6%,following by C.tropicalis(13.8%),C.parapsilosis(10.3%),C.glablate(8.6%)and C.krusei(4.3%).Drug resistance to four commonly used antifungal drugs showed drug sensitivity to amphotericin B and itraconazole was higher,while that to fluconazole and flucytosine was lower.CONCLUSIONS Significant difference of drug resistance occurs among invasive fungus species.The cross resistance to azole antifungals is existing and has a raising tendency.

Objective To evaluate the role of IL-2 dendritic cell in chronic hepatitis B,peri-dendritic cells were cultured,cytokins CD4O,CD86,CD80,CD83 and IL-2 were measured by ELLISA.Methods 20 chronic hepatitis B patients were selected,each one donated 15ml blood.dentritic cells were cultured,and IL-4 1000u/ml rhGM-CSF 1000u/ml was given each well,while IL-2 100u/ml was given each control well.CD40,CD86,CD80 and CD83 were measured by FLSC at 9th day.IL-2 was detected by ELISA at 12th day.Results The level of CD40,CD86,CD80 and CD83 in increased when using IL-2,and dentritic cell secreted IL-2 in chronic hepatitis.Conclusion The results suggest that IL-2 can promote the phenotypic and functional maturation of PC.

Small molecule antibodies are naturally existed and well functioned but not structurally related to the conventional antibodies. They are only composed of heavy protein chains or light chains, much smaller than common antibody. The first small molecule antibody, called Nanobody was engineered from heavy-chain antibodies found in camelids. Cartilaginous fishes also have heavy-chain antibodies (IgNAR, "immunoglobulin new antigen receptor"), from which single-domain antibodies called Vnar fragments can be obtained. In addition, free light chain (FLC) antibodies in human bodies are being developed as therapeutic and diagnostic agents. Comparing to intact antibodies, common advantages of small molecule antibodies are with better solubility, tissue penetration, stability towards heat and enzymes, and comparatively low production costs. This article reviews the structural characteristics and mechanism of action of the Nanobody, IgNAR and FLC.

Breast tumor is the most common malignant tumor in female,its incidence is increasing in recent years.With magnetic resonance equipment,image acquisition and processing technology is improved,perfusion imaging studies gradually become the focus of study.The author reviewed MRI perfusion imaging and its application in breast tumors.