OBJECTIVE:To study the mechanism that apoptosis of NB4 cells were induced by artesunate. METHODS: The apoptosis of NB4 cell induced by artesunate was determined by Annexin V-FITC FCM analysis and cytomorphology and the activity of Caspase-3 was determined by ELISA assay. RESULTS: Artesunate could induce apoptosis of NB4 cell with the activity of Caspase-3 increasing. CONCLUSIONS: The apoptosis of NB4 cell induced by artesunate is achieved by activating Caspase-3.

The deficiencies in reading popularization at present were analyzed, the concepts of liberal education and liberal platform were described, and the successful experiences in liberal platform-based reading popularization in academic libraries were summarized, such as experiencing human library by stressing on communication between users, achieving the reading and education effect by applying the liberal concept, selecting reading materials based on the needs of teachers and students, and establishing special reading popularization team.

The contents, methods and commons of social information service in Markerere University Library were analyzed according to the social information service in domestic academic libraries and the enlightenments on extending social information service in domestic academic libraries were elaborated, such as active participating in study of social problems and attaching importance to librarians training .

Objective Compared with surgical operation,to investigate the clinical efficacy and advantages of interventional treatment in blunt liver traum.Methods 32 patients from July 2010 to October 2012,who were diagnosed as blunt liver trauma,received super selective hepatic artery embolization in Chinese People's Liberation Army in 98th Hospital.Another 27 ones received operation at the same time in Peolies's Liberation Army 98th (AAST grade Ⅱ to Ⅳ).Retrospective analysis of related cases was presented.The results were analyzed by t test.Results All of 32 patients that received super selective hepatic artery embolization were stanched bleeding successfully.All patients were followed up for 3 to 18 months,the mean follow-up time was 10 months,with no severe complications occurred or no lost cases.Conclusions Compared with surgical operation,interventional treatment had advantage that included eliable hemostasis,less operation time,minimally invasive and less hospitalization time.Interventional treatment had good effects on blunt liver trauma which were AAST grade Ⅱ to Ⅲ.

At present, there are 8 sources of neural stem calls (NSCs), such as the early embryo, umbilical cord blood, brain tissue of adults and bone marrow, which are used for experiments. Four methods are also provided to isolate NSCs, for example, the serial passage and the gene transduction. Most of the hotspots at home and abroad are on the modulation of NSC transplantation in vivo, to make it proliferate and differentiate in hand. Most findings are achieved through experiments on rodents, no matter in vitro or vivo, which indicate that the proliferation and differentiation of NSCs can be affected by a variety of biological factors and drugs. Even more, the effects are multiple according to the factors and drugs of different kinds and the same kind of different concentrations, even the same factor would have different roles in different process of the culture of NSCs. The survival of NSCs during transplantation can also be affected by local microenvironment. Neither the NSC endogenous nor exogenous treatment is efficient. Furthermore, the body also rejects the exogenous NSCs. Taken together, it is tough for us to conclude that a factor does have some effects on the NSCs. Moreover, there are still a series of key problems unveiled clinically, such as how to perfect transplantation and whether to choose exogenous or endogenous NSCa for transplantation.

The paper analyzes the current research on ontology related to biomedicine based on literature review through investiga-tions on related literatures. Seven hot points are elaborates by co - word analysis and cluster analysis, including the conduction of gene expression, protein - related research, development of ontology - related system and software, related research on gene and chromo-some, subject heading research related ontology, methodology research on computational biology and genomics, gene expression re-search.

ObjectiveTo explore the prognosis and relationship between soluble differentiation antigen of cluster designation 14 (sCD14) and the injury severity score (ISS) of casualties with multiple injuries.Methods A total of 86 casualties with multiple injuries were enrolled from October 2009 to March 2010,and the severity of trauma of casualties were assessed in ISS score within the first 24 hours after accident,and the patients were divided into survival and non-survival groups as per the outcomes in 28 days after accident. Another 20 healthy subjects served as control group. In multiple injuries group,sCD14 concentrations were detected and APACHE Ⅱ scores were calculated on the 1st,3rd,5th and 7th days after accident.The related coefficient between sCD14 and ISS was calculated and then the values of sCD14 in predicting prognosis were analyzed by ROC curve. ResultsCompared with control group, sCD14 concentrations were obviously higher in casualties of multiple injuries group at all observation intervals ( P ＜0.01). Compared with survival group, casualties of non-survival group had more higher sCD14 concentrations and APACHE Ⅱ scores ( P ＜ 0.05 ).The sCD14 concentration was correlated with ISS and the related coefficient was 0.469 ( P ＜ 0.01 ). ROC curve analysis suggested sCD14 had values in predicting prognosis of casualties with multiple injuries and area under ROC curve was 0.820.Conclusions The concentration of sCD14 is correlated with ISS and can be used for predicting the prognosis of casualties with multiple injuries.

BACKGROUND:The effect of placenta-derived growth factor(PLGF)on promoting vascular regeneration remains uncertain.In studies of models with PLGF deletion,PLGF has been considered to play critical role in inducing angiogenesis.OBJECTIVE:To summarize the research advances in effect of PLGF and its receptor in vascular regeneration and ischemic cerebral disease.METHODS:A computer-based online search of Pubmed and CNKl was performed for relevant articles published between 1990 and 2009 with key words of"PLGF"in Chinese and"PLGF angiogensis"Articles related with biological characteristics of PLGF and its roles in vascular regeneration and articles related with angiogenesis and PLGF expression after cerebral ischemia were selected.Repetitive articles were excludedRESULTS AND CONCLUSION:The role of PLGF and its receptor in vascular regeneration,mechanism of PLGF and its receptor to promote angiogenesis,vascular endothelial growth factor A,and the expression of PLGF and its receptor in brain after cerebral ischemia were introduced.PLGF can promote pathological angiogenesis,arteriogenesis,branch formation and hemopoietic progenitor cell mobilization.PLGF and its receptor play an important role in the angiogenesis of ischemic cerebral disease and display a good clinical prospect in the treatment of ischemic cerebral disease However,the specific role and mechanism require further study.

Acupuncture Therapy ; instrumentation ; Adolescent ; Adult ; Aged ; Facial Paralysis ; therapy ; Female ; Humans ; Male ; Middle Aged ; Young Adult

Objective To study the problems in clinical practice of nursing students and their experience in the operating room.Methods Interview,on-the-spot recording and note-taking were used in the investigation among 16 nursing students at their clinic internship in the operating room.The data were analyzed by way of type analyzing method.Result Four factors were found, including un-adaptability to the clinical environment,distance between nursing students and teachers,lack of high-tech,and emotional experience.Conclusion The nursing teachers should make clear teaching aim,reduce their mistakes in the work in the operation room,and attach more importance to the cultivation of humanistic quality and interpersonal communications.