Objective To observe the efficacy of bioelectric stimulation of the pelvic floor combined with acupuncture in treating detrusor urine weakness after transurethral resection of the prostate (TURP) in patients with prostate hyperplasia.Methods Forty-seven cases with detrusor urine weakness after TURP were randomly divided into an experimental group and a control group.All of the patients received routine bladder function training and medication.In addition,patients in the experimental group were treated with bioelectric stimulation of the pelvic floor and with acupuncture.The differences between the two groups were compared using the International Prostate Symptom Score (IPSS),maximum urine flow rate (MFR),average flow rate (AFR),detrusor pressure at peak flow (PdetQmax) and residual urine volume in the bladder.Results No significant difference was found between the two groups before treatment,but at two weeks after treatment MFR,AFR and PdetQmax were significantly higher in the experimental group than in the control group,and residual urine volume and IPSS were significantly lower.Conclusion Bioelectric stimulation of the pelvic floor combined with acupuncture is more effective than routine bladder function training and medication alone for alleviating detrusor urine weakness after TURP.

OBJECTIVE:To establish the quality standard of Tangkang capsule. METHODS:Momordica charantia L. and Euonymus alatus(Thunb.)Sieb.,the main ingredients in Tangkang capsule including were identified by TLC, meanwhile, the content of total Momordica charantian saponin was determined by UV-Vis. RESULTS: The TLC spots were clear and the separation degree was good. The linear range of Ginsenoside Rg1 was 72～252 ?g(r=0.999 7) with average recovery rate at 100.68% (RSD=1.43%,n=6). CONCLUSION: The established quality standard can be used for the quality control of this Tangkang capsule.

Objective To explore the methods of first-staged operation and its clinical efficacy in the carcinoma of left colon with obstruction.Methods The clinical datas of 15 cases of patients hospitalized in 1995 to 2005 were retrospectively analyzed.Results Incision infection and liquification occurred in 4 cases.No patient had severe complications such as fistula in anastomosis and infection of abdominal cavity.Conclusion The therapy of incison and anastomosis in the first-staged operation is practicable if we strictly control indications and improve procedures between the pre-and post-operation.

Objective To compare the clinical effect of single-row (SR) and double-row (DR)rotator cuff injury repair.Methods Twenty-three patients with rotator cuff injury were randomized into SR group(12 patients) and DR group (11 patients) by ranom digits table.Two anchors and simple sutures were applied in SR group,three anchors and medial mattress sutures were applied in DR group.Clinical function were compared after operation 24 months,included pain visual analogue scale (VAS) scores,Constant scores,American Shoulder and Elbow Surgeons (ASES) scores,University of California at Los Angeles (UCLA) scores,range of shoulder motion (anteflexion,adtorsion conclination,external rotation),operation time and patient's satisfaction were compared.Re-tear rate and clinical function were also analyzed.Results The operation time in SR group was shorter than that in DR group [(115.8 t25.0) min vs.(124.5 ± 19.7)min,P =0.033].The scores of VAS,Constant,ASES,UCLA in two groups had no significant difference (P ＞ 0.05).The full-thickness re-tear in SR group was 2 patients,in DR group was 2 patients,there was no significant difference (P ＞ 0.05).The full-thickness re-tear and part-thickness re-tear in SR group was 7 patients,in DR group was 2 patients,there was significant difference(P=0.041).Furthermore,the scores of VAS,Constant,ASES,UCLA had no significant difference (P＞ 0.05).Conclusion Compared with SR,2-5 cm rotator cuff injury repaired by DR has no obviously advantage.

Objective To investigate the fluorescence quantitative polymerase chain reaction and teat its diagnostic value of DNA(EBV-DNA)of EB virus in respiratory tract infection in children.Methods To adopt fluorescent quantitative PCR technology(FQ-PCR)to examine EBV-DNA in the aspirate specimen from 127 cases of children.Results 68 cases of EBV-DNA positive(53.5％),including 1year of age:12;3 years of age:23;5 years of age:18;7 years of age:10;9 years of age:3 and 13 years of age:2.The number of peripheral white blood cells ＞ 10.0× 109/L:21 cases;the number of white blood cells ＜ 10.0 × 109/L:8 cases;the number of mononuclear cells ＞ 10％:9 cases;abnormal lymphocyte ＞ 10％:10 cases;thrombocytopenia:4 cases;abnormal liver fumction:5 cases; myocardial enzyme abnormalities:8 cases.Conclusion The fluorescent quantitative PCR technology to test EB-DBA of respiratory tract infection in children had hight diagnostic value.

OBJECTIVE: To analyze the pathogenesis and clinical manifestations of delayed encephalopathy after acute carbon monoxide poisoning, and to investigate the method of complex treatment and the factors influencing prognosis.DATA SOURCE: Related papers were searched by computer in Chinese Journal Full-text Database (CJFD) from January 1983 to October 2000 and CBMdise with the terms of "acute, carbon monoxide poisoning, delayed,encephalopathy", and the language of paper was limited to Chinese.STUDY SELECTION: The papers relevant to the pathogenesis, clinical manifestation, treatment and prognosis of acute carbon monoxide poisoning were collected preliming screening. Inclusion criteria: ① Randomized controlled trial. ② Blind method was not restricted. Exclusion criteria: The same researches repeated.DATA EXTRACTION: A total of 240 pieces of paper related to acute carbon monoxide poisoning induced eneephalopathy were collected, among which, 24 pieces accorded with inclusion criteria.DATA SYNTHESIS: The pathogenesis of acute carbon monoxide poisoning induced encephalopathy was relatively complex, which should be deeply recognized from the clinical manifestations such as symptom, sign,electroencephalogram, cerebral CT and MRL The therapeutic methods included ①Neural cell agonist, vasodilator, anticoagulant and vitamin ②High pressure oxygen ③ Ultraviolet blood irradation and oxygenation, UBIO. ④ Acupuncture therapy. The factors influencing prognosis of delayed encephalopathy after acute carbon monoxide poisoning were usually related to age, degree of poisoning, complications and cure time.CONCLUSION: Acute carbon monoxide poisoning can cause encephalopathy, which is the result of multiple pathogenesis. There is no special treatment for it until now, and complex treatment should be used, but the curative effect is not ideal, and intellectual disturbance and extremity function disorder of different degree might be left after treatment, and agrypnocoma would exist for a long time.

Electromagnetic Fields ; adverse effects ; Humans ; Occupational Exposure ; analysis ; Railroads

Humans ; Occupational Exposure ; Railroads

Objective:To investigate the dissdution behavior of Gansukang capsules from different manufactures in two different dissdution media and to provide theoretical basis for evaluating the quality of medicine. Methods:According to the dissolution test con-ditions in Japan’ s “quality of medical drugs information set” and the physicochemical properties of Gansukang capsules, two dissolu-tion media were chosen. The dissolution behavior of Gansukang capsules from 3 manufacturers in pH 6. 8 phosphate buffer (containing 0. 25% sodium lauryl sulfate) and water (containing 0. 25% sodium lauryl sulfate) was determined by the method I in Appendix XC of Chinese Pharmacopoeia Volume II (2010 edition), and the dissolution rate was determined by HPLC-ELSD. Results:The assay dis-played a good linearity over the concentration range of 2. 0-40. 0 mg·ml-1 for oleanolic acid, the recovery of oleanolic acid in pH 6. 8 phosphate buffer and water was above 98%, and Gansukang capsules had the similar property in the two media. The dissolution behav-ior of Gansukang capsules from 3 manufacturers in water was similar to each other, while in pH 6. 8 phosphate buffer, that was signifi-cantly different. Conclusion:As for the dissolution medium for Gansukang capsule, pH 6. 8 phosphate buffer can reflect more differ-ences in the dissolution of products with different batches and from different manufacturers than water. The method is simple, accurate and reproducible in the dissolution determination of the capsules.

Objective:To investigate the effect of transfection with wild hepaCAM gene on biological behavior of the transitional cell carcinoma of bladder in vitro.Methods:Human TCCB cell line T24 was transfected with pEGFP-N2-hepaCAM,mock plasmid pEGFP-N2 with lipofectamine.Laser confocal microscopy was used to detect the location of hepaCAM in cells.Western bolt was used to determine the expression of target of hepaCAM in stable cells.The cell adhesive and motility ability were tested by cell spreading assay and matrigel invasion assay.Cell proliferation ability was investigated by MTT assay。Results:In well spread cells,hepaCAM localized on the perinuclear membrane,whereas in the cells which contacts,hepaCAM predominantly accumulated at the sites of cell-cell contacts on cell membrane.Cell clone with stable expression of hepaCAM,was acquired.In vitro experiments as cell spreading assays and matrigel invasion assays showed the cell adhesion and cell motility properties of hepaCAM group were apparently enhanced compared with the non-transfection or mock-vehicle groups.The MTT assay showed cell proliferation ability in the hepaCAM group was notably decreased when compared with the non-transfection or mock-vehicle groups.Conclusion:The hepaCAM gene can restrain malignant phenotypes of the human TCCB cells in vitro,and may inhibit the TCCB invasion and metastasis by influencing the function of some adherence and proliferation factors.[