Objective To study the effect of methotrexate on the neointimal formation after arterial balloon injury. Methods Male rabbits were randomized into four groups: MTX 0.5 mg/kg per week, MTX 1.5 mg/kg per week, MTX 5 mg/kg per week and the control. Drugs were administered by intramuscular injection. Rabbit carotid arteries were harvested 2 and 4 weeks after injury. Results Histologically, the intimal areas were reduced significantly in MTX treated animals compared with the controls. The VSMC proliferation in injured vessels was identified by immunostaining for proliferating cell nuclear antigen (PCNA). In comparison, PCNA-positive cells in both intima and media were significantly reduced by treatment of MTX. But MTX did not enhance reendothelialization in the injured carotid arteries as determined by Evans blue stain.Conclusion Low dosage of MTX could attenuate neointimal formation after arterial injury by inhibiting VSMC proliferation.

Objective To acquire high spatial resolution two-dimension pulse pressure distribution for objectifying research of pulse diagnosis in traditional Chinese medicine.Methods A sensor array with flexible packing was designed and implemented.The array was formed on a circle substrate with 37 silicon piezoresistive pressure sensors,which were distributed as equilateral triangles with 1.95 mm pitch.The diameter of the substrate was 15 mm.By using flexible packing,the array had the hardness similar to that of human finger,which made this method closer to the traditional Chinese diagnosis.After differential amplifying,37-way pulse pressure signals were digitalized and reconstructed to obtain the pulse pressure distribution.Results With the application of the sensor array,the pulse pressure signals were measured and transformed into pulse pressure distribution.Conclusions The experimental result contains the relevant characteristics of traditional Chinese pulse diagnosis' description,providing a new way for objective study of traditional Chinese pulse diagnosis and further study.

Female ; Humans ; Infant, Newborn ; Mandibulofacial Dysostosis

Objective To study and observe the effect of astragalus membranaceus on serum free amino acids and chemokines in patients with rheumatoid arthritis. Methods 60 patients with rheumatoid arthritis in Lishui Hospital of traditional Chinese medicine from October 2014 to May 2016 were selected as the research object,and 60 patients were divided into control group and observation group with 30 cases in each group,according to the method of random number table,the control group were treated with conventional treatment of rheumatoid arthritis,the observation group were treated with astragalus mongholicus on the basic of control group,then the serum free amino acids and chemokines of two groups before the treatment and at 10th,20th,40th and 60th day after the treatment were respectively detected and compared. Results The serum free amino acids and chemokines of two groups before the treatment were compared, while the serum chemokines of observation group at 10th,20th,40th and 60th day after the treatment were all lower than those of control group,the serum free amino acids glutamic acid,asparagic acid and phenylalanine were all lower than those of control group,leucine,γ-aminobutyric acid, glycine and taurine were all higher than those of control group,the difference was statistically significant (P<0.05). Conclusion The influence of astragalus mongholicus for the serum free amino acids and chemokines of patients with rheumatoid arthritis is better,so its application in the patients with rheumatoid arthritis is higher.

Objective To study a correlativity of amplitude of low frequency fluctuation change of restingstate brain activity and aphasia quotient in aphasia patients after stroke and investigate recovery and mechanism of the aphasia.Methods Adopting amplitude of low-frequency fluctuation (ALFF) in blood oxygenation level dependent functional MRI(BOLD-fMRI) and Siemens version 3.0T MR Scanner was used to obtain 12 aphasia patients and 20 normal volunteers of fMRI data.The fMRI data were processed with the software of DPARSF and analyzed by ALFF,and group analysis was performed with two sample t-test by REST software to obtain increased and decreased ALFF map.Brain regions,in which,ALFF of aphasia group was lower than that of normal control group,were done correlation analysis with aphasia quotient.Results As compared with those in normal subjects,the regions showing decreased ALFF in aphasia patients were distributed in left middle temporal gyrus,left medial prefrontal gyrus,right cerebellum,which were positively correlated with aphasia quotient (r Roi1 =0.48,r Roi2 =0.36,r Roi3 =0.28,respectively);while the regions showing increased ALFF covered left occipital lobe,precentral gyrus,insula,right precuneus.Conclusion As compared with those in normal subjects,the regions positively correlated with aphasia quotient show decreased ALFF in aphasia patients,which are one of the mechanisms of aphasia.The regions increased ALFF in aphasia patients may participate in language function of recovery.

Purpose To explore the relationship between the mutations of epidermal growth factor receptor ( EGFR) and KRAS genes and clinicopathological characteristics in patients with non-small cell lung cancers (NSCLC). Methods Clinical samples from 431 NSCLC patients were obtained for EGFR and KRAS gene analysis. PCR based direct DNA sequencing was used to investigate mutations in exon 18-21 of EGFR gene and codon 12 and 13 of exon 2 of KRAS gene. Results The overall EGFR mutation rate of primary NSCLC was 53. 6% (231/431) in this study cohort and eight cases showed double EGFR mutations. Mutation rates in female and male were 65. 2% (122/187) and 46. 9% (98/209), respectively. The mutation rate was higher in patients with non-smokers and adeno-carcinoma and adenosquamous carcinoma subtypes than in their counterparts (P<0. 05), with the percentage of 57. 2% (124/216), 60. 3% (199/330), 42. 9% (6/14), respectively. In squamous cell carcinomas and other subtypes, EGFR mutation rates were 11. 6% (5/43) and 11. 1% (1/9), respectively. The EGFR mutation types included exon 18 point mutations (4. 0%, 9/227), exon 19 deletion mutations (4. 5%, 101/227), exon 20 insert or point mutations (9. 7%, 22/227) and exon 21 point mutations (41. 4%, 94/227). Activating mutations of KRAS gene were detected in 7. 8%(31/396) of NSCLC. Twenty-eight patients showed codon 12 mutations ( G>T, G>A, G>C) , and three patients had codon 13 mutations ( G>A, G>T) . Most of these mutations were G to T transversion (64. 5%, 20/31). Conclusion Polymerase chain reaction-direct sequencing is a reliable and effective method for the detection of the EGFR and KRAS gene mutation in NSCLC patients. The mutation rate of EGFR is higher in Chinese patients, especial-ly in non-smoking female patients with adenocarcinoma.

Purpose To analyze the image quality of brain CT with 256-slice wide detector axial scanning mode,routine axial scanning mode and spiral scanning mode,and to provide a more effective brain CT examination method for patients.Materials and Methods The prospective study was conducted on 90 patients accepting routine brain CT examination,and they were randomly divided into three groups.CT examination with 160 mm axial scanning mode,40 mm axial scanning mode and 40 mm spiral scanning mode were respectively conducted using GE Revolution CT.The scanning condition was adjusted to remain constant radiation dose,and then the image quality was analyzed.CT attenuation of gray and white matter,contrast-to-noise ratio (CNR) of white-gray matter and image noise of the three scanning modes were compared.Subjective scoring on image quality of the three scanning modes was also performed.Results On body lateral cerebral ventricle level,there were no significant difference in CT attenuation of gray and white matter and CNR (P＞0.05).On centrum semiovale level,the CT attenuation of gray matter [(31.71 ± 1.82) HU],white matter [(22.97± 1.50) HU] and CNR 2.05±0.42 of 160 mm axial scanning mode was significantly different from the other two scanning modes (F=26.74,47.16 and 3.85,P＜0.05).On centmm semiovale level,image noise of 160 mm axial scanning mode was lower than the other two kinds of scanning methods (F=6.31,P＜0.05),in the basal ganglia and posterior fossa there were no statistically significant differences in the image noise between the three scanning modes (P＞0.05).The subjective score of the three scanning modes all met the diagnostic requirements,and there was no significant difference (P＞0.05).The effective dose and scanning time of 160 mm axial scanning mode was lower than those of the other two scanning modes,and the X-ray utilization was higher.Conclusion 160 mm wide detector axial scanning mode is more suitable for brain CT scan,and it can be used as the preferred scanning mode in the emergency and among non-cooperative patients.

AIM: To investigate the clinical effect of vitamin B12 eye drops for vision fatigue caused by visual display terminals (VDT).
METHODS: Totally 50 patients (100 eyes) with vision fatigue caused by VDT were averagely divided into two groups. The control group were treated with normal saline,the treatment group were treated with vitamin B12 eye drops, 3 times per day, one drop each time, continuous for 60d. Accommodative parameters and Schirmer Ⅰtest were measured and analyzed before and after treatment.
RESULTS: After treatment, the results of Schirmer Ⅰtest, accommodative amplitude and accommodative facility of the treatment group were higher than those of the control group ( all P < 0. 05 ). And the results of accommodation lag of the treatment group were lower than those of the control group (P<0. 05).
CONCLUSION: Vitamin B12 eye drops can lessen symptoms of dry eye, improve accommodative function and treat vision fatigue caused by VDT.

Animals ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; methods ; Multiple Organ Failure ; complications ; drug therapy ; prevention & control ; Phytotherapy ; Systemic Inflammatory Response Syndrome ; complications ; drug therapy ; prevention & control

Objective:To discuss the therapy for ureteral endometriosis.Methods:The clinical data of 25 cases of histopathologically confirmed ureteral endometriosis during 2001 -2015 were retrospectively analyxed.Results:In the 25 cases,all the patients took urinary ultrasound for examination before sur-gery,of whom 21 (84%)were examined by CT and 5 (20%)by MRI.Three (12%)cases underwent preoperative KUB and intravenous pyelogram (IVP)examination.Four (16%)cases were examined by retrograde pyelography before surgery.Eight (32%)of them took cystoscope for examination and 11 (44%)took preoperative radionuclide renal dynamic imaging examination.All of these cases were affec-ted with unilateral ureter,13 (52%)in the right and 12 (48%)in the left.The ureter lesions were at the upper part in 21 (84%)cases and at the middle part in 4 (16%)cases.In these cases,11 (44%) received partial ureteral resection and end-to-end ureteral anastomosis,10 (40%)received partial ure-teral resection and ureterocystoneostomy,3 (12%)received retroperitoneal laparoscopic nephroureterec-tomy,and 1 (4%)received endoscopic resection of ureteral endometriosis lesion.All of these cases were confirmed with ureteral endometriosis by post-surgery pathology results,with the expression of CA125 and ER in the glandular tissue and expression of PR in the mesenchymal tissue inside the ureteral muscle de-tected by immunohistochemistry.Four (16%)cases took postoperative adjuvant hormonal therapy.And no recurrence was found among 16 (64%)cases with the successful follow-up which ranged from 3 to 76 months and the median follow up was 28 months.Fifteen cases were submitted to the follow-up by urinary ultrasound (93.8%)and 5 (31.3%)underwent CT for examination.All the cases relieved from hydro-nephrosis,and symptoms of 10 (63.5 )cases disappeared.The cases with double-J stent all had the stent removed within 3 to 6 weeks.Conclusion:Surgical procedures should be considered as the main therapy for ureteral endometriosis.We recommend ureterolysis for patients with mild ureteral obstruction and hydronephrosis.As for those with moderate and severe ureteral obstruction and hydronephrosis,we recommend partial ureteral resection.When the situation comes to patients with little renal function of the affected side,the recommended management is nephroureterectomy.