Epithelial-mesenchymal transition (EMT),which means the transformation of epithelial cells into mesenchymal cells under the influence of cytokines,leading to pathological changes in tissues.According to recent research findings,EMT plays an important role in or-gan fibrosis.Various types of EMT in the fibrosis of hepatobiliary system,as well as the characteristics of each stage and the disease progres-sion,are described,which is of great significance for understanding the development,termination,and even reversion of EMT and fibrosis of hepatic and biliary epithelial cells.

As an independent subject, TCM psychology appeared in 1980s, but it could be traced back to the book, the Inner Canon of Huangdi (Huangdineijing) formed in Qin and Han Dynasties. Physicians in the later generations developed TCM psychology based on that occurred in the Inner Canon of Huangdi, which made it became mature gradually, and Sun Simiao was one representative figure among them. The fundamental features of TCM psychological theory of Sun Simiao are summarized here.

This study was aimed to observe the influence of Kang-Xian Y i-Xin (KXYX) formula on the heart size and cardiac function of patients with dilated cardiomyopathy (DCM). A total of 85 cases were randomly divided into the treatment group (43 cases) and the control group (42 cases, with 1 death case). Both groups were treated with routine western medicine. And the KXYX formula was combined in the treatment group once a day. Six months later, changes of the left ventricular end diastolic diameter (LVEDD), left atrial diameter (LAd), ejection fraction (EF) and fractional shortening (FS) were observed by ultrasonography. The results showed that both groups can reduce the LVEDD and Lad. And the effect was obvious in the treatment group (P< 0.05). There was significant difference in the LVEDD of both groups after treatment (P< 0.05). The EF and FS were raised obviously in both groups. There was significant difference before and after treatment in the treatment group (P < 0.01). There was statistical differ-ence between groups after treatment (P< 0.05). It was concluded that the KXYX formula can decrease the LVEDD, Lad, enhance EF and FS, in order to promote the cardiac function of patients with DCM.

EML4-ALK fusion gene is a new molecular type of non-small cell lung cancer,which is formed by inversion of two gene segments on chromosome 2.Up to now,9 subtypes have been found and all of them have deteriorated and carcinogenic abilities. The detection rate of this fusion gene is much higher in patients who have adenocarcinoma,never or seldom smoke and lack EGFR/KRAS mutation.This fusion gene codes ALK tyrosine kinase,that abnormally phosphorylates downstream products through signaling pathways,leading to canceration.Therefore,the specific inhibitors which target this fusion gene could inhibit its expression and have good treatment effect.At present,the relevent inhibitors which are undergoing clinical trials are PF02341066 and TAE684.

ObjectiveTo investigate the expression of psoriasin in actinic keratosis(AK),Bowen's disease and squamous cell carcinoma(SCC) tissues.MethodsImmunohistochemistry was carried out to quantify the expression of psoriasin protein in tissue specimens from the lesions of 20 patients with AK,25 patients with Bowen' s disease,21 patients with well differentiated SCC,and16 patients with poorly differentiated SCC,as well as from the skin of 18 normal human controls.ResultsThe expression rate of psoriasin was 11.1％ in the control specimens,significantly different from that in the AK(95.0％),Bowen's disease (88.0％),well differentiated SCC (95.2％),and poorly differentiated SCC (92.3％) specimens (all P ＜0.01 ).Psoriasin was expressed in the cytoplasm of keratinocytes and dyskeratotic keratinocytes in stratum corneum and upper 1 to 3 layers of stratum spinosums in AK tissue,in the cytoplasm of keratinocytes as well as the membrane and cytoplasm of vacuolated cells in all layers of the epidermis in Bowen's disease tissue.In well differentiated SCC,cornified pearl and dyskeratotic keratinocytes located in the stratum comeum and all layers of the stratum spinosums stained positive for psoriasin,and in poorly differentiated SCC,psoriasin was distributed in the stratum corneum and upper 1 to 5 layers of the stratum spinosums,but absent in poorly differentiated squamous cells.The expression intensity of psoriasin in tissues sequentially increased from AK,Bowen's disease to well differentiated SCC.Though the expression intensity of psoriasin in poorly differentiated SCC decreased,but was still higher than that in normal skin tissue(P ＜ 0.05).ConclusionPsoriasin is abnormally expressed in skin disorders with dysdifferentiation of squamous cells.

Human herpesvirus-8 (HHV-8) also referred to as Kaposi’s sarcoma-associated herpes-virus (KSHV),is a novel tumor causing virus,which is thought to be the etiologic agent of KS,and also is associated with primary effusion lymphoma(PEL) and multicentric castleman’s disease(MCD) HHV-8 encodes a number of viral proteins, including the proteins associated with HHV-8 latent infection and lytic infection,and the proteins unique to HHV-8,which are critical players in the development of KS and HHV-8-associated diseases. The proteins encoded by HHV-8 and association between HHV-8 and KS were reviewed.

Objective To investigate the risk factors of cervical cancer in Chinese married women in recent 10 years in order to provide evidence based approaches for cervical cancer prevention and control Methods Eight case-control studies from 2002 to 2011 were selected from research literatures by using keywords such as cervical cancer, risk factors, influential factors and case-control study, cancer, risk factors, factors andcase-control study as the search term.We adopted the Mentel-Haensel fixed effect model and Dersimonion-Laird random effect model to gain a comprehensive and quantitative assessment of cervical cancer and its risk factors.Results Among the 8 case-control studies,the total number of cases and controls were 2868 and 8045,respectively.The risk factors included human papilloma virus (HPV) (RR =5.47,95 ％ CI:3.40-8.82),family history of cervical cancer (RR =2.40,95 ％ CI:1.39-4.16),number of abortions (RR =1.74,95％ CI:1.49-2.03),first sexual intercourse age number of sexual partners (1.72,95％ CI:1.36-2.16),low cultural level (RR =1.68,95％ CI:1.18-2.40).Conclusion The major risk factors for cervical cancer among married women in China included HPV,family history of cervical cancer,number of abortions,first sexual intercourse age number of sexual partners and low cultural level.

Objective To explore the method and efifcacy of intervention aortopulmonary septal defect (APSD) in young infant. Methods A four month old APSD infant had undergone interventional treatment using homemade ventricular septal de-fect occluder, and was followed up to observe the curative effect. Results The APSD had been successfully closured, and the clinical symptoms improved instantly. The follow-up showed heart function improved, heart shadow reduced, and growth well. Conclusions APSD interventional therapy is a safe, effective treatment in young infant.

Objective To study of effects Medlar flavone on NO and NOS in H 2 O2 damaged vascular endothelial cells.Meth-ods Human Umbilical Vein Endothelial Cells(HUVEC)have been set as research object.HUVEC cells was damaged by H 2 O2 in-duced oxidative stress.Experiments were divided into normal control group,H 2 O2 injury group,VitC control group,and low,medi-um,high flavonoids protective groups(100,200,400 mg/L).MTT method was used to observe cells activity.The effects Medlar fla-vone on MDA、TNOS、iNOS and NO in H 2 O2 damaged vascular endothelial cells was observed.Results (1)MTT result demonstra-ted the IR of HUVEC cells was 38.8% in H 2 O2 injury group.Which in low,medium,high flavonoids protective groups were 34.0%,30.7%,25.5%.All were lower than H 2 O2 injury group(P <0.01).(2)Compared with normal control group,NOS activity was decreased in H 2 O2 injury cell,while TNOS,iNOS,NO was more.Compared H 2 O2 group,the change of NO,TNOS,iNOS, MDA was opposite in flavone intervention group,and which were dose dependent (P <0.01 ).Conclusion Medlar flavone has a protective effect on H 2 O2 induced HUVEC cells injury,and there may be some kind of relevance between them.

Objective To explore the differentiation of neural stem cells (NSCs) in hippocampus of rat model of Parkinson's disease (PD).Methods Rat models of PD were made by injection 6-hydroxydopamine (6-OHDA) into striatum. 5-bromodeoxyuridine (Brdu) was injected into the abdomens of PD rats for 14 days before they were killed. The expressions of Brdu/Glial fibrillary acidic protein (GFAP) and Brdu/ Neuronal nuclear antigen (Neun) were determined by immunocytochemistry. The distinctive marker for Brdu/GFAP and Brdu/Neun labeling method was used to determine the phenotype of the differentiated cells.Results After PD models were established, the Brdu/GFAP and Brdu/Neun positive cells appeared at 7 d, increased gradually at 14 d, and peaked at 28 d. In these double labelled cells, Brdu/GFAP positive cells were more than Brdu/Neun positive cells.Conclusions Direct injection of 6-OHDA into the striatum stimulates the proliferation of inherent NSCs in hippocampus of rats. Large amounts of NSCs differentiate into astrocytes and small amounts of them turn into neurons.