In this article,suggestions about how to set up basic computer courses system in medical universities are brought forward.In addition,how to give prominence to emphases after teaching reform of computer and researches and reform of teaching mode of computer courses are discussed.

This article briefly introduced the concept and classification of assistive products for persons with disability, the development of assistive products in the developed countries as well as several types of new representative assistive products.

Humans ; Liver, Artificial

OBJECTIVE To investigate the bacterial distribution and the extent of drug resistance in lower respiratory tract disease among aged people.METHODS From Jan 2006 to Dec 2006 we collected 304 sputum samples of the old patients who were diagnosed lower respiratory tract infection,bacterial distribution and antibiotic resistance of 178 strains of the pathogens were analyzed retrospectively.RESULTS The most common four kinds of Gram-negative bacilli were Pseudomonas aeruginosa,Klebsiella pneumoniae,Escherichia coli and Acinetobacter baumannii.For above bacteria,the ratio of the resistance to ampicillin,cefazolin and compound sulfamethoxazole was over 40%.The isolating rate of ESBLs-producing E.coli and K.pneumoniae was 29.8%.CONCLUSIONS Monitoring the common bacteria and ESBLs bacteria precisely can provide therapy,guide to early cure and control lower respiratory tract infection of aged patients effectively.

Objective To investigate the effects of antisense oligonucleotide against miRNA-155 (AS-miRNA-155) on proliferation,apoptosis and invasion and migration abilities of human cutaneous squamous cell carcinoma cell line A431. Methods AS-miRNA-155 was transfected into human cutaneous squamous cell carcinoma A431 cells by using LipofectamineTM 2000. Blank control without transfection and transfected with non-sense sequence were used as non-sense sequence control. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression of miRNA-155 in A431 cells. Cell proliferation was analyzed using dimethyl thiazolyldiphenyl tetrazolium (MTT) assay. Cell cycle arrest and apoptosis were studied by flow cytometry (FCM). Invasion and migration were measured by Transwell chamber assays. Results The relative expression of miRNA-155 mRNA was lower in the transfection group than that in the blank control group and the negative control group (F=634.57, P<0.01), but there was no significant difference between the blank control group and the negative control group. After 72 h transfection, the survival rate was significantly lower in the transfection group than that of the blank control group and the negative control group, and the transfection rate decreased significantly by 120 h (P<0.05). Cells of G0/G1 phase increased, Cells of S phase reduced, the overall PI value decreased in transfection group, and the apoptosis rate of A431 cells, migration and invasion of cells increased (P<0.05). There was no significant difference in G2/M cycle between transfection group, blank control group and negative control group. There were no significant differences in A431 cell apoptosis rate, cell migration and invasive ability between blank control group and negative control group. Conclusion Antisense oligonucleotide against miRNA-155 can inhibit the expression of miRNA-155, the proliferation and promote the apoptosis of human cutaneous squamous cell carcinoma A431 cells, which indicates that miRNA-155 may become a new target for the regulation of gene expression in cutaneous squamous cell carcinoma.

The antimicrobial abuse has become an indisputable fact as well as a global public health concern.At the 2016 G20 summit in Hangzhou,the issue of antimicrobial resistance was also put on the agenda of world leaders.This paper analyzed the status quo of global antimicrobial resistance.With reference to the report Tackling drug-resistant infections globally: final report and recommendation written by British Lord Jim O′Nell published in May 2016,the main influencing factors of antimicrobial resistance were described.In addition,this paper proposed countermeasures for antimicrobial resistance,including carrying out large-scale promotional activities,improving global public health conditions,reducing antimicrobial use in agriculture,strengthening the global surveillance of antimicrobial resistance,developing new treatment products for infectious diseases,and building a new pattern of global response.

Studies on theory and clinic of massage in treating lumbar disc herniation were usually done fome aspects of traditional meridians and acupoints theory or modern biomechanics theory,few was down on basis of the guidance of musculature theory for massage in treating lumbar disc herniation.In fact,it was used generally by civilian doctors and minor ethnic doctors in clinic.So,this article discussed the guidance of musculature theory for massage in treating lumbar disc herniation from following aspects:basic concept of musculature theory,the understanding on etiopathogenisis and pathogenesis of lumbar disc herniation in musculature theory,the selection of massage location and manipulation maneuver,etc.

Objective To analyse pathogens distribution and antibacterial susceptibility of bacteria isolated from clinical speci‐mens in neurosurgery ,in order to provide laboratory basis for rational use of antibacterial agents .Methods Isolation and culturing of bacteria were carried out in various clinical samples collected in neurosurgery from January 2012 to September 2012 in the First Affiliated Hospital of Xi′an Jiaotong University ,all strains were identified by using automatic microbial analyzer ,and the sensitivi‐ties to antibacterial agents were verified by using instrument method and K‐B method .WHONET5 .6 software was used for data a‐nalysis .Results A total of 1 319 strains of non‐repeating pathogenic bacteria were isolated ,including 482 strains (36 .6% ) of gram‐positive bacteria ,797 strains (60 .4% ) of gram‐negative bacteria ,and 40 strains (3 .0% ) of fungi .Most of pathogenic bacteria were isolated from sputum ,cerebrospinal fluid ,urine and blood specimens ,and the most common isolates were Staphylococcus aureus , Acinetobacter baumannii ,Klebsiella pneumoniae ,Pseudomonas aeruginosa and Escherichia coli .Isolated gram‐positive bacteria and gram‐negative bacteria were resistant to antibacterial agents in different degree .The sensitivity rate was 100 .0% only in linezolid and vancomycin against Staphylococcus aureus .The resistant rates of carbapenems against Acinetobacter baumannii were higher than 80 .0% ,and carbapenems‐resistant Klebsiella pneumoniae strains had also appeared .Carbapenems‐resistant Escherichia coli strains had not yet been found .The sensitive rate of Piperacillin‐Tazobactam against Pseudomonas aeruginosa was above 90 .0% . Conclusion Gram‐negative bacteria is the predominant pathogen infecting patients in neurosurgery ,w hile Staphylococcus aureus are the most common isolates .For the increase of multiple drug‐resistant and pan‐drug‐resistant bacteria ,clinical rational use of antibac‐terial agents for anti‐infection treatment may be very important .

Objective:Based on the hospital resource planning(HRP) system, design in delicacy maintenance information management system of medical equipment, aims to solve problems for preventive maintenance, repair decision support, repairing parts management, fault library, practical training, cost analysis, workload statistics and other aspects.Methods: The five issues including establishment of a standardized procedure for solving maintenance, electronic document management, scientific and material support, comprehensive personnel management, cost analysis, statistical and intelligent decision support by the Visual Basic for application programming technology were solved to realize the database interaction. Constructing standardization process of equipment maintenance management and making full use of the stored information can provide guidance for equipment maintenance management activities.Results: The equipment maintenance management and integrated decision analysis, provides a strong reference for all types of decision-making of the hospitals. To realize information sharing among departments make hospital equipments maintenance comply with a standardized and scientific management requirements.Conclusion: It can be widely applied in hospital equipment maintenance management process and is significant to the achievement of scientific management of medical equipment.

OBJECTIVE:To establish a HPLC method for determination of the content of baicalin in Bifukang oral liq?uid.METHODS:The ODS C 18 column was used with methanol-water-glacial acetic acid(45∶55∶1.5)as mobile phase,and de?tective wavelength was274nm,the flow rate was1ml/min.RESULTS:Within the range of0.215?g～4.3?g,baicalin presented a fine linear relationship between amount and peak area(r=0.9995).The average recovery was99.44%(RSD=0.79%,n=5). CONCLUSION:This HPLC method is convenient,rapid and accurate,and can be used for quality control in the production of Bifukang oral liquid.