1.INDUCTION OF MYOCARDIUM CELLS FROM P19 CELLS USING DIMETHYL SULFOXIDE IN VITRO
Acta Anatomica Sinica 1954;0(02):-
Objective Observing the process of cell aggregation and differentiating into myocardium after the P19 cells treated with different methods and dimethyl sulfoxide(DMSO) inducer.Methods P19 cells were cultivated with DMSO in suspension for 7 days in Petri dishes or dishes containing a thin layer of soft agar to form cell aggregation.Then the aggregates were plated on gelatin-coated culture dishes,and cultured without DMSO to 19 days.The beating of cells was observed.?-sarcomeric actin and cardiac Troponin T(cTnT) immunocytochemical stains were used to identify cell differentiation.Results Cell aggregations were cohering-cultured with growth culture medium after DMSO induced for 7days,spontaneous and rhythmically beating cells were present within the aggregation outgrowths at day 15,which were ?-sarcomeric actin-positive and cTnT-positive.By day 19,positive rates were about 26% and 15% respectively.Conclusion By using DMSO exposure combination prior aggregation can induce P19 cells differentiate into cardiac myocytes which can spontaneously and rhythmically beat.
2.Knowledge mapping of journal Military Medicine
Bulletin of The Academy of Military Medical Sciences 2009;33(6):513-518
Objective:To grasp the macro trend of the U.S.military medical advances through the knowledge mapping of 2 966 records in journal Military Medicine.Methods:CiteSpace Ⅱ running on Java platform was used.Results:Military medicine was closely related to federal medicine in the U.S.,and this relation was definite and clear.The missions of the U.S.military medical research institutions were different and were not obviously overlapping.A number of national agencies and military agencies as well as some experts formed the solid foundation for the development of military medicine. Key literature of military medicine could be visually displayed. The U.S. military medicine focused on combat casualties, PTSD, veteran affairs, dental emergencies and health promotion.Conclusion:Novelty, necessity and applicability of the knowledge mapping analysis method are displayed in the field of military medicine.
6.Surgeon's decision upon pathological judgement.
Chinese Journal of Surgery 2009;47(20):1523-1524
7.A primary study of evolution of hepatitis B virus based on motif discovery.
Lei MA ; Qing-Qing YI ; Qi ZHANG ; Jian-Feng HE
Chinese Journal of Virology 2014;30(1):13-18
Hepatitis B is a serious infectious disease worldwide, and hepatitis B virus (HBV) is the direct cause of this disease. In recent years, as an essential part of its evolutionary process, HBV mutation has been extensively studied domestically and globally. However, the study on the conserved sequences in HBV sequences is still in its infancy. In this study, we applied multiple EM for motif elicitation (MEME) algorithm to discover HBV motif and proposed a new metric, conservative index (CI), to carry out phylogenetic analysis based on HBV sequences. Then, the constructed phylogenetic tree was subjected to reliability assessment. The results demonstrated that the new metric CI combined with the MEME algorithm can effectively help to discover motifs in HBV sequences and construct a phylogenetic tree based on them and to analyze the evolutionary relationship between HBV sequences; in addition, the possible ancestral sequences of samples may be obtained by conservative analysis. The proposed method is valuable for the exploratory study on large HBV sequence data sets.
Computational Biology
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Conserved Sequence
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Evolution, Molecular
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Hepatitis B virus
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genetics
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Humans
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Nucleotide Motifs
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Phylogeny
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Reproducibility of Results
8.Expression of MMP-9 and TIMP-1 in ectopic and eutopic endometrium of adenomyosis treated with mifepristone
lei, CAI ; qing, YUAN ; wen-qing, LONG ; duan-duan, LA
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(08):-
Objective To study whether the mechanism of mifepristone in treating adenomyosis is suppressing matrix metalloproteinase-9 and tissue inhibitors of metalloproteinase-1(MMP-9/TIMP-1). Methods Thirty-five patients in the mifepristone treated group(19 cases of adenomyosis) and the control group(16 cases of adenomyosis,non-drug treated) underwent hysterectomy.Endometrium was looked as eutopic endometrium and adenomyosis as ectopic endometrium.Expression of MMP-9 and TIMP-1 in eutopic and ectopic endometrium were measured by immunohistochemical techniques. Results The ectopic endometrium of the mifepristone treated group expressed lower level of MMP-9,higher level of TIMP-1 and lower ratio of MMP-9/TIMP-1 than the ectopic endometrium of the control group(P
9.Screening and Identification of Bacillus fusiformis Bioconverting Isoeugenol to Vanillin
Li-Qing ZHAO ; Lei-Lei ZHU ; Zhi-Hao SUN ;
Microbiology 1992;0(01):-
Using isoeugenol as the sole carbon source,a novel strain,producing high amounts of vanillin from isoeugenol,was isolated from soil.According to the physiological and biochemical characteristics and its 16S rRNA gene sequence analysis,it was identified as Bacillus fusiformis.The initial results showed that 4.20 g/L vanillin was obtained by bioconversion of 2% isoeugenol with Bacillus fusiformis.