Objective: To study the effect of esomeprazole in treating elderly patients with NSAIDs correlated peptic ulcer bleeding. Methods: Patients with NSAIDs correlated peptic ulcer bleeding in our hospital from January, 2010-December, 2012 were enrolled and randomly divided into observation group given esomeprazole and control group given omeprazole. Then the baseline data were surveyed and effect of ulcer bleeding, condition of abdominal pain alleviation and ulcer ulcer, adverse reaction cases were observed. Results: (1)there were no significant difference between the baseline data of two group;(P>0.05);(2)in observation group, excellence rate was 42/70(60%), effective rate was 24/70(34.3%), the overall treatment effect was better than that of control group, the difference had statistical significant(P<0.05);(3)there were no significant difference between two group of abdominal pain and ulcer healing;(4) during treatment, there were no serious adverse reactions in the two groups. Conclusion:Compared with omeprazole, esomeprazole has equal effect on abdominal pain relief and ulcer healing, and it is much more effective in treating elderly patients with NSAIDs correlated peptic ulcer bleeding.

Premixed insulin is currently the most popular insulin starter in China.According to the different ratios of basal and prandial components,premixed insulin analogues could be classified into low-and midmixtures.The former is now mainly used as insulin starter in the patients with type 2 diabetes mellitus after the failure of monotherapy or combination with oral antidiabetic drugs,while the later is usually used as simplified intensive insulin treatment in patients with type 2 diabetes mellitus inadequately controlled on insulin once-or twice-daily.

Pyroptosis is a newly discovered form of programmed cell death, which has a close relationship with infectious diseases and spontaneous inflammatory disease and autoimmune disease.Gasdermin-D is the effector of pyroptosis.Inflammatory caspases cleave Gasdermin-D, break the structural autoinhibition and active the pore-forming activity of Gasdermin-D.Gasdermin-D-N domain is the active form of Gasdermin-D.Oligomerized Gasdermin-D-N domain forms membrane pores.Both pyroptosis and necroptosis can form pores.In this article, the progress of the research on pyroptosis, the involvement of Gasdermin-D in the molecular mechanism of pyroptosis, the distinguishment of pyroptosis from necroptosis and pyroptosis-related diseases are reviewed.

Objective To realize centralized supply of hospital compressed air to reduce repeate investment.Methods Air turned into compressed one after passing through air pressure pump, which undergoes preliminary cooling and drying by refrigerated drier, then the compressed air went into air tank for further cooling and storage, and finally was transmitted into hyperbaric oxygen chamber, ICU and operating room as desired.Results Centralized supply was realized for hospital compressed air with little technical modification and investment.Conclusion The compressed air of hyperbaric oxygen chamber is introduced into ICU and operating room and gains economic and social benefits, and thus is worth popularizing practically.

ObjectiveTo investigate the clinicalefficacy of warm needling plus Chinese herb fumigation in treating plantar fasciitis.MethodSixty-one patients with plantar fasciitis were randomly allocated to a treatment group of 31 cases and a control group of 30 cases. The treatment group receivedwarm needling plus Chinese herb fumigation and the control group, warm needling alone.ResultThe total efficacy rate and the cure rate were 100.0% and 61.3%, respectively, in the treatment group and 96.7%and 40.0%, respectively, in the control group. There was no statistically significant difference in the total efficacy rate between the two groups (P>0.05), but the cure rate was significantly higher in the treatment group than in the control group and there was a statistically significant difference between the two groups (P<0.05). There was a statistically significant pre-/post-treatment difference in the VAS score in the two groups (P<0.01), indicating that both the groups had a good analgesic effect. There was a statistically significant post-treatment difference in the VAS score between the treatment and control groups (P<0.01), indicating that theanalgesic effect was better in the treatment group than in the control group.ConclusionThe efficacy of warm needling plus Chinese herb fumigation is better than that of warm needling alone in treating plantar fasciitis.

Objective To investigate the intervention of Chinese herb Shengjiang San(SJS)for the imbal-ances of serum Th1/Th2 cells and related regulatory factors in sepsis patients. Methods Fifty-five sepsis patients were randomly divided into two groups:conventional treatment group of 27 cases and SJS group of 28 cases. Nine cases of healthy volunteers were enrolled as control group in the study. Cases of conventional group were treated with western medicine only and cases of SJS group were treated with both western medicine and Chinese herb SJS (100 mL twice one day). The therapy course of both groups was 3 days. Score of Chinese medical syndromes ,se-rum level of leukocyte count,C reactive protein(CRP)and procalcitonin(PCT),serum T-bet,GATA-3,Th1&Th2 cells in the proportion of whole CD4+Th cells and Th1/Th2 ratio were compared respectively in each group be-fore and after treatment. Results There were significant differences between SJS group and conventional group in score of Chinese medical syndromes,serum level of leukocyte count,T-bet,GATA-3,Th1&Th2 cells in the pro-portion of whole CD4+Th cells and Th1/Th2 ratio(P<0.05 or P<0.01). There were no significant differences be-tween the two groups in serum level of CRP,PCT and GATA3. Compared with control group,there were signifi-cant differences in all indicators except GATA3 of the two groups(P<0.01). Conclusion Chinese herb Shengji-ang San has an effective benefits to Chinese medical syndromes,inflammatory reaction,the imbalance of serum Th1/T2 and related regulatory factors(T-bet&GATA-3)in sepsis patients.

Micro-syringe as a result of the high frequency of clinical use, direct contact with patients, the failure of its equipment will have a direct impact on the patient's disease status. Researchers in this paper, by simulating the test fluid, electrical safety testing and analysis of statistical methods such as maintenance records, consider six kinds of common injection pump, in terms of performance, safety and the failure rate of three performance. Compared the performance analysis, summarized the various types of injection pump characteristics, researchers make suggestions to clinical departments, equipment procurement and equipment management departments for usage and improvement.
Equipment Failure ; Equipment Safety ; Syringes

Objective To evaluate the clinical outcome of the technique of step-by-step in situ rod bending used in posterior internal fixation of lumbar burst fractures.Methods A retrospective case control analysis was conducted on 76 cases of lumbar burst fractures hospitalized between March 2007 and March 2014.There were 48 males and 28 females,with a mean age of 40.4 years (range,18-57 years).Fractured segments included L1 in 32 cases,L2 in 24,L3 in 14 and L4 in 6.According to the different rod bending methods,the subjects were divided into preliminary rod bending group (34 cases) and in suit rod bending group (42 cases).Operation time,amount of bleeding during and after operation,restoration of anterior vertebral height and Cobb angle,and visual analogue score (VAS) were compared between groups.Results Seventy patients were followed up for 12-36 months (mean,20.9 months).One patient in preliminary rod bending group presented internal fixation loosening,and had implant removal 9 months after operation.No implant breakage occurred.Comparison of preliminary rod bending group and in situ rod bending group showed significant differences in operation time [(88.1 ± 16.0)min vs.(79.9 ± 14.7)min],amount of bleeding during operation [(88.3 ± 21.2) ml vs.(74.0 ± 12.5) ml],amount of bleeding after operation [(71.5 ± 17.3)ml vs.(62.1 ± 16.0) ml],restoration of anterior vertebral height [(74.1 ±7.8) ％ vs.(79.5 ± 8.5) ％],and restoration of Cobb angle [(10.2 ± 2.7) °vs.(8.8 ± 2.6) °] (P ＜0.01 or 0.05).VAS presented no significant differences between preliminary rod bending group and in situ rod bending group before operation [(7.5 ± 0.9) points vs.(7.2 ± 1.0)points] and 1 week after operation [(2.9 ± 0.7) points vs.(3.0 ± 0.7) points] (P ＞ 0.05),while there was significant difference 9 months after operation [2.2±0.7)points vs.(1.9 ±0.7)points] (P＜0.01).Conclusion In suit rod bending for lumbar burst fractures has advantages over preliminary rod bending in terms of operation time,blood loss,precise restoration and back pain.

Objective To construct a lentiviral expression vector of murine RelB for RNAi,and then to effectively silence the RelB gene expression of murine bone marrow derived dendritic cells for constructing the bone marrow tolerogenic dendritic cell,in order to provide an experimental foundation for a novel clinical therapy of autoimmune disease.Methods RelB shRNA sequence of mouse was designed by on-line designer software on Corp.Invitrogen,after synthesis and annealing,double strand oligonucleotides(dsoligoes)were cloned into the pENTRTM/U6 plasmid,then after sequencing,a positive clone was further subcloned into pLenti6/BLOCK-iTTM-DEST vector.It was then transformed into stb13 competent cell,and after sequencing,293FT cell line was transfected by above positive recombined plasmid and lentiviral packing materials.It was incubated for 48 to 72 h in a 37℃,5% CO2 incubator.Culture supernatant was harvested and stored at-80℃,then the virus titer was determined by serial dilution assay.Results It was showed from sequencing figures that all the pENTRTM/U6-RelB-shRNA plasmids were positive clone vector,and this positive recombinant vector was recombined with pLenti6/BLOCK-iTTM/U6-DEST vector.It was then transformed into stb13 competent cell and screen positive clone by ampicillin,and resequenced by the use of primer forward U6 primer.The results also showed that recombinant lentiviral vector was positive clone.Viral particle was packaged with other packaging material mediated by lipofectamine 2000 in 293FT cell line.Cultural supernatant was collected and stored at-80℃,and lentiviral particle titer was determined by serial dilution assay with 6?105/transduced unit.Conclusion Lentiviral shRNA expression vector of murine RelB gene for RNAi was successfully constructed.It might be a rational procedure to make tolerogenic DC,and then to develop a DC vaccine and DC-based immunotherapy for autoimmune diseases.

Objective To explore a simple and effective method of culturing primary human bronchial epithelial cells.Methods Bronchial epithelial cells were obtained from human bronchial tissues obtained from lobectomy or pneumectomy.Epithelial cells were isolated by three methods as fellows:1.proteinase XIV digestion with mechanical scraping,2.proteinase XIV digestion with mechanical stripping,and 3.trypsin digestion with mechanical scraping.Subsequently the cells were cultured in serum-free hormone-supplemented DMEM/F12.The quantity,purity and viability of cells isolated by three methods were compared.The cultured cells were identified with cytokeratins AE1/AE3 by immunohistochemistry.Results The quantity of cells [(9.37?1.62)?105] obtained from proteinase XIV digestion with mechanical scraping was significantly higher than that of proteinase XIV digestion with mechanical stripping [(5.20?0.75)?105](P