BACKGROUND:Stem cel transplantation is a promising strategy for treatment of ischemic heart diseases, which has obtained great achievements in recent years. OBJECTIVE: To analyze the clinical trends of stem cel therapy for ischemic heart diseases reported from 2001 to 2015 in Medline, CNKI, Wanfang, ClinicalTrials.gov and Chinese Clinical Trial Register. METHODS:The relevant articles addressing stem cel therapy for ischemic heart diseases were retrieved using the keywords of “stem cel transplantation” and “ischemic heart disease” in Chinese and English in Medline, CNKI, Wanfang, ClinicalTrials.gov and Chinese Clinical Trial Register folowed by statistical analysis. The retrieval time was from 2001 to 2015, including 2001-2005, 2006-2010 and 2011-2015. RESULTS AND CONCLUSION: In Medline database, there were 219 clinical studies about stem cel therapy for ischemic heart disease published from 2001 to 2015, and the number of retrieved articles was most from 2006 to 2010, and decreased from 2011 to 2015. In CNKI and Wanfang databases, the number of relevant articles which had a similar trend with that in the Medline database decreased remarkably after 2011. In ClinicalTrials.gov, there were 63 clinical trials about stem cel therapy for ischemic heart disease, most of which were registered from 2006 to 2015 and came from Europe and North America. In Chinese Clinical Trial Register, there was no clinical trial about stem cel therapy for ischemic heart disease, which may result from the strict management in China.

Objective To investigate the cause of postoperative colorectal cancer anastomotic fistula,the treatment and prevention measures.Methods 2007 -2014,The department of General surgery in Qingdao Municipal hospitals for abdominal colorectal cancer resection(Dixon surgery)were 208 cases,including 13 cases of anastomotic leakage occurred.Retrospective summarized the cause,treatment and preventive measures of abdominal colorectal cancer anastomotic fistula.Results All of 13 patients were discharged,and had no adhesions obstruction,anastomotic stricture,pelvic abscess and other symptoms following -up 6 months after discharge.Conclusion Grasping preopera-tive risk factors of abdominal colorectal cancer for anastomotic fistula,actively preoperative preparation,and master of fine anatomical and surgical point are the main points of the prevention of anastomotic leakage.

Objective To study the effects of severely burned rats serum on migration of BMSCs and mechanism.Methods Severely burned rats model was established,and the preparation of severely burned rats serum.Experimental groups:normal train-ing group(containing 10% fetal bovine serum,group C),burn serum group(containing 10% burns in the rat serum,group B),burn serum+blockers(10% burns in the rat serum+final concentration of 10 μmol/L PI3K signaling pathway inhibitor LY294002 train-ing,group B+LY).Activity of cells was examined with MTT;migration of cells was examined with Transwell chambers testing;protein expression of p-AKT/AKT was determined with Western blot;microtubule structure of cells was examined with immuno-fluorescence.Results Compared with group C,group B burn serum treatment after 24 h,BMSCs activity(P <0.01),p-AKT levels (P <0.05),increased migration quantity(P <0.001);cell microtubule structures appear rupture,after adding inhibitor,compared with group B,group B+LY BMSCs activity(P <0.01),to reduce the number of migration(P <0.001),p-lower AKT(P <0.05), cell microtubule structure similar to the normal group.Conclusion Severely burned rats serum can promote BMSCs migration,may burn serum cytokine activation of PI3K/AKT signal pathway,resulting in cell microtubule structure change,promote the migration of BMSCs.

The degradation kinetics of chlorogenic acid (5-CQA), cryptochlorogenic acid (4-CQA), and neochlorogenic acid (3-CQA) in aqueous solution at 37 degrees C and different pH values (7.05, 7.96, 9.25) were investigated in the present work. The results indicated that 3-, 4- and 5-CQA tended to remain stable in acidic pH circumstance, and unstable in neutral and alkaline pH circumstance. With the increase of the alkalinity, the degradation of 3-, 4- and 5-CQA was increased leading to a less amount of total CQA and was satisfactorily described by the Weibull equation. Meanwhile, caffeic acid was not detected after the degradation of CQA. Moreover, the degradation of 3-CQA and 5-CQA tended to be converted to 4-CQA, and the degradation of 4-CQA tended to be converted to 3-CQA rather than 5-CQA. The comparison of the degradation kinetics parameters of 3-, 4- and 5-CQA at neutral and alkaline pH values showed that the orders of the rate constant (k) values were 4-CQA > 3-CQA > 5-CQA, while the orders of the degradation half life (t½) values were 4-CQA < 3-CQA < 5-CQA, indicating the orders of the stabilities of 3-, 4- and 5-CQA at 37 degrees C and neutral and alkaline pH values were 4-CQA < 3-CQA < 5-CQA.
Caffeic Acids ; Chlorogenic Acid ; analogs & derivatives ; chemistry ; Hydrogen-Ion Concentration ; Kinetics

Preventing and curing emergency infectious diseases,such as A H1N1 influenza,is a public health practice,and its relevant ethical problems are pubic health ethical problems,mainly manifesting as the conflicts between individual interests and public interests,individual freedom and quarantine,individual privacy right and releasing partial medical information,and choosing one's own doctors and compulsory treatment.The core of the issue is that individual interests should be submitted to the public interests,and at the same time government and society should respect individual's freedom,privacy and right of health.

OBJECTIVE: To investigate the compatible stability of cefotiam hydroloride for injection with fructose injection.METHODS: The content changes of the mixture within 8h at 25℃ were determined by UV spectrophotometry,and its external appearance was observed,and the pH value as well as particle size of the mixture were measured.RESULTS: The mixture was clear within 6h,showing no visible changes in appearance,pH value and contents of cefotiam,with particles in line with the standard stated in China Pharmacopeia(2005 edition).CONCLUSION: The mixture of ceforiam hydrochloride for injection and fructose injection is stable within 6h.

s: Recently, following the development of basic experimental research, great progress has been acquired in the fields of the understanding and management of the acute coronary syndromes (ACS). This review summarizes the molecular and cellular bases, the management of the unstable plaque, risk stratification and the management of ACS.

Objective:To investigate the expression of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor-C (VEGF-C) in papillary thyroid carcinoma and their relationship to cervical lymph metastases.Method:In this study, the expressions of COX-2 and VEGF-C were examined by immunohistochemistry in papillary thyroid carcinoma tissues of 40 patients, and analysis was performed on the correlation of cervical lymph metastases with COX-2 and VEGF-C expression.Result:Positive expressions of COX-2 and VEGF-C were 70.0%(28/40)and 75.0%(30/40)respectively in papillary thyroid carcinoma. The positive rates of COX-2 and VEGF-C expression were 80.8%(21/26)and 84.6%(22/26)respectively in patients with cervical lymph metastases, and 50.0%(7/14)and 57.1%(8/14)respectively in patients without cervical lymph metastases, with a statistically significant difference between two groups(P<0.05, all). COX-2 was positively correlated to VEGF-C expression in papillary thyroid carcinoma(r=0.378, P<0.05).Conclusion:The results suggest that COX-2 and VEGF-C were highly expressed in papillary thyroid carcinoma, with possible interaction of their expressions, and may play a critical role in the cervical lymph metastases of papillary thyroid carcinoma.

Objective To assess the characteristics of heart rate turbulencer (HRT) in elderly patients with decreased left ventricular diastolie function.Methods 40 patients were divided into two groups:20 patients with enlarged left atrium and 20 patients without enlarged left atrium.20 healthy people were selected as controls. Turbu1ence onset (TO) and turbulence slope (TS) were measured,and correlation was analyzed between TO,TS and the E/A,index of Macruz and heart rate variability (HRV).Results TO was higher (P<0.05) and TS was lower in patients (P<0.01),TO was higber in patients with enlarged left atrium than in with out enlarged lef atrium people (P<0.05) and TS was lower (P<0.01).TO was positively (P<0.01) and TS Was negatively (P<0.05),correlated with the index of Macruz.TO Was negatively (P<0.05) and TS was positively (P<0.05) related to SDNN and SDANN.Conclusion HRT can be used as a potential risk predictor in decreased left ventricular diastolic function patients.

Objective To study the anti-vertigo function of polysaccharides of Gastrodia elata (GEP) and polysaccharides of Armillaria mellea (AMP). Methods Regard vertigo mice caused by machinery rotation as research object, observe the escaping time of electrical shock in maze experiment and jumping platform test, and observe the food intake. Results GEP and AMP can obviously shorten the escaping time of electrical shock (P