Objective To observe the efficacy of bioelectric stimulation of the pelvic floor combined with acupuncture in treating detrusor urine weakness after transurethral resection of the prostate (TURP) in patients with prostate hyperplasia.Methods Forty-seven cases with detrusor urine weakness after TURP were randomly divided into an experimental group and a control group.All of the patients received routine bladder function training and medication.In addition,patients in the experimental group were treated with bioelectric stimulation of the pelvic floor and with acupuncture.The differences between the two groups were compared using the International Prostate Symptom Score (IPSS),maximum urine flow rate (MFR),average flow rate (AFR),detrusor pressure at peak flow (PdetQmax) and residual urine volume in the bladder.Results No significant difference was found between the two groups before treatment,but at two weeks after treatment MFR,AFR and PdetQmax were significantly higher in the experimental group than in the control group,and residual urine volume and IPSS were significantly lower.Conclusion Bioelectric stimulation of the pelvic floor combined with acupuncture is more effective than routine bladder function training and medication alone for alleviating detrusor urine weakness after TURP.

OBJECTIVE:To establish the quality standard of Tangkang capsule. METHODS:Momordica charantia L. and Euonymus alatus(Thunb.)Sieb.,the main ingredients in Tangkang capsule including were identified by TLC, meanwhile, the content of total Momordica charantian saponin was determined by UV-Vis. RESULTS: The TLC spots were clear and the separation degree was good. The linear range of Ginsenoside Rg1 was 72～252 ?g(r=0.999 7) with average recovery rate at 100.68% (RSD=1.43%,n=6). CONCLUSION: The established quality standard can be used for the quality control of this Tangkang capsule.

Objective To compare the clinical effect of single-row (SR) and double-row (DR)rotator cuff injury repair.Methods Twenty-three patients with rotator cuff injury were randomized into SR group(12 patients) and DR group (11 patients) by ranom digits table.Two anchors and simple sutures were applied in SR group,three anchors and medial mattress sutures were applied in DR group.Clinical function were compared after operation 24 months,included pain visual analogue scale (VAS) scores,Constant scores,American Shoulder and Elbow Surgeons (ASES) scores,University of California at Los Angeles (UCLA) scores,range of shoulder motion (anteflexion,adtorsion conclination,external rotation),operation time and patient's satisfaction were compared.Re-tear rate and clinical function were also analyzed.Results The operation time in SR group was shorter than that in DR group [(115.8 t25.0) min vs.(124.5 ± 19.7)min,P =0.033].The scores of VAS,Constant,ASES,UCLA in two groups had no significant difference (P ＞ 0.05).The full-thickness re-tear in SR group was 2 patients,in DR group was 2 patients,there was no significant difference (P ＞ 0.05).The full-thickness re-tear and part-thickness re-tear in SR group was 7 patients,in DR group was 2 patients,there was significant difference(P=0.041).Furthermore,the scores of VAS,Constant,ASES,UCLA had no significant difference (P＞ 0.05).Conclusion Compared with SR,2-5 cm rotator cuff injury repaired by DR has no obviously advantage.

Objective To investigate the fluorescence quantitative polymerase chain reaction and teat its diagnostic value of DNA(EBV-DNA)of EB virus in respiratory tract infection in children.Methods To adopt fluorescent quantitative PCR technology(FQ-PCR)to examine EBV-DNA in the aspirate specimen from 127 cases of children.Results 68 cases of EBV-DNA positive(53.5％),including 1year of age:12;3 years of age:23;5 years of age:18;7 years of age:10;9 years of age:3 and 13 years of age:2.The number of peripheral white blood cells ＞ 10.0× 109/L:21 cases;the number of white blood cells ＜ 10.0 × 109/L:8 cases;the number of mononuclear cells ＞ 10％:9 cases;abnormal lymphocyte ＞ 10％:10 cases;thrombocytopenia:4 cases;abnormal liver fumction:5 cases; myocardial enzyme abnormalities:8 cases.Conclusion The fluorescent quantitative PCR technology to test EB-DBA of respiratory tract infection in children had hight diagnostic value.

Objective To explore the clinical effect and safety of levosimendan,milrinone,and dobutamine in treatment of patients with ADHF.Methods All 336 patients with ADHF accepted in Xi'an Center Hospital from April 2013 to April 2016 were selected and divided into group A,B,and C with 112 cases in each group.Patients in three groups were given levosimendan,milrinone,and dobutamine respectively.Then the clinical effect,hemodynamics,serum indexes,and safety of two groups were observed and compared.Results The total efficacy of group A (90.18％) was obviously higher than group B (58.93％) and group C (65.18％) with statistically significance (P ＜ 0.05).PCWP,PAMP,RAP,and SVR of three groups after treatment were lower than before (P ＜ 0.05).And PCWP,PAMP,and SVR of group A were obviously lower than those of group B and C (P ＜ 0.05).RAP among three groups after treatment had no difference.NT-proBNP,ET-1,and NE of three groups after treatment were lower than before (P ＜ 0.05),and group A was obviously lower than group B and C (P ＜ 0.05).Adverse reaction rate of group A was obviously lower than that of group B and C with statistically difference (P ＜ 0.05).Conclusion Levosimendan has better effect and safety than milrinone and dobutamine in treatment of patients with ADHF,which is worth clinical application.

The formation causes of medical disputes in the department of medical registration are analyzed from the aspects of doctors,registration personnel,patients and computer operational system.Furthermore,some feasible countermeasures are also put forward as well.

Humans ; Occupational Exposure ; Railroads

Objective To explore the methods of first-staged operation and its clinical efficacy in the carcinoma of left colon with obstruction.Methods The clinical datas of 15 cases of patients hospitalized in 1995 to 2005 were retrospectively analyzed.Results Incision infection and liquification occurred in 4 cases.No patient had severe complications such as fistula in anastomosis and infection of abdominal cavity.Conclusion The therapy of incison and anastomosis in the first-staged operation is practicable if we strictly control indications and improve procedures between the pre-and post-operation.

Objective:To investigate the effect of transfection with wild hepaCAM gene on biological behavior of the transitional cell carcinoma of bladder in vitro.Methods:Human TCCB cell line T24 was transfected with pEGFP-N2-hepaCAM,mock plasmid pEGFP-N2 with lipofectamine.Laser confocal microscopy was used to detect the location of hepaCAM in cells.Western bolt was used to determine the expression of target of hepaCAM in stable cells.The cell adhesive and motility ability were tested by cell spreading assay and matrigel invasion assay.Cell proliferation ability was investigated by MTT assay。Results:In well spread cells,hepaCAM localized on the perinuclear membrane,whereas in the cells which contacts,hepaCAM predominantly accumulated at the sites of cell-cell contacts on cell membrane.Cell clone with stable expression of hepaCAM,was acquired.In vitro experiments as cell spreading assays and matrigel invasion assays showed the cell adhesion and cell motility properties of hepaCAM group were apparently enhanced compared with the non-transfection or mock-vehicle groups.The MTT assay showed cell proliferation ability in the hepaCAM group was notably decreased when compared with the non-transfection or mock-vehicle groups.Conclusion:The hepaCAM gene can restrain malignant phenotypes of the human TCCB cells in vitro,and may inhibit the TCCB invasion and metastasis by influencing the function of some adherence and proliferation factors.[

Objective: to study the relationship between immune complex(IC)and the origin of atherosclerosis(AS),and to evaluate the simvastatin's potential effect on immunomodulation. Methods: Thirty eight truebred New Zealand rabbits were randomly divided into four groups: control group,high-cholesterol fed group,simvastatin-protecting group and simvastatin-treatment group.the structural change of artery and the deposit of IC(and their classes)in plaque lesions of AS were analyzed with hematoxylin and eosin stain and immunohistochemistry. Results: plaque lesions of AS were obvious after 8-weeks high-cholesterol feeding.With the development of AS,the IC of IgG accumulated in plaque lesions,while the IC of IgM did not.The immunohistochemical stain was stronger in high-cholesterol feed group than that in the simvastain-intervening groups.The distribution of immune complexes was under the endothelium in the early plaque while mainly localized in the core or the shoulder of mature plaque lesions. Conclusion: The IC of IgG exists in the plaque lesions of AS.The IC is close related to the origin and the progress of AS.Simvastatin can inhibit the formation of IC as well as lowering the lipid levels and regressing the plaque,which maybe related to it's effect on the immunomodulation.