Objective To observe the efficacy of bioelectric stimulation of the pelvic floor combined with acupuncture in treating detrusor urine weakness after transurethral resection of the prostate (TURP) in patients with prostate hyperplasia.Methods Forty-seven cases with detrusor urine weakness after TURP were randomly divided into an experimental group and a control group.All of the patients received routine bladder function training and medication.In addition,patients in the experimental group were treated with bioelectric stimulation of the pelvic floor and with acupuncture.The differences between the two groups were compared using the International Prostate Symptom Score (IPSS),maximum urine flow rate (MFR),average flow rate (AFR),detrusor pressure at peak flow (PdetQmax) and residual urine volume in the bladder.Results No significant difference was found between the two groups before treatment,but at two weeks after treatment MFR,AFR and PdetQmax were significantly higher in the experimental group than in the control group,and residual urine volume and IPSS were significantly lower.Conclusion Bioelectric stimulation of the pelvic floor combined with acupuncture is more effective than routine bladder function training and medication alone for alleviating detrusor urine weakness after TURP.

Objective　To investigate the causes of recurrent spontaneous abortion(RSA) and to know the relation between RSA and antiphospholipid thrombosis syndrome(APL-T). Methods　ELISA and PTT-LA were used to detect anticardiolipin antibody(ACA) and lupus anticoagulant(LA) in 32 patients with RSA and 20 normal controls(NC). Results　There were 17 positive of antiphospholipid antibody(APA) in RSA group. The incidence was significantly higher than that in NC group. The incidence of LA was also significantly higher than that in NC group. 8 patients were diagnosed as APL-T. Conclusion　LA may be the more important reason of RSA. It should be considered as APL-T when RSA is unexplained.

OBJECTIVE:To establish the quality standard of Tangkang capsule. METHODS:Momordica charantia L. and Euonymus alatus(Thunb.)Sieb.,the main ingredients in Tangkang capsule including were identified by TLC, meanwhile, the content of total Momordica charantian saponin was determined by UV-Vis. RESULTS: The TLC spots were clear and the separation degree was good. The linear range of Ginsenoside Rg1 was 72～252 ?g(r=0.999 7) with average recovery rate at 100.68% (RSD=1.43%,n=6). CONCLUSION: The established quality standard can be used for the quality control of this Tangkang capsule.

Objective Compared with surgical operation,to investigate the clinical efficacy and advantages of interventional treatment in blunt liver traum.Methods 32 patients from July 2010 to October 2012,who were diagnosed as blunt liver trauma,received super selective hepatic artery embolization in Chinese People's Liberation Army in 98th Hospital.Another 27 ones received operation at the same time in Peolies's Liberation Army 98th (AAST grade Ⅱ to Ⅳ).Retrospective analysis of related cases was presented.The results were analyzed by t test.Results All of 32 patients that received super selective hepatic artery embolization were stanched bleeding successfully.All patients were followed up for 3 to 18 months,the mean follow-up time was 10 months,with no severe complications occurred or no lost cases.Conclusions Compared with surgical operation,interventional treatment had advantage that included eliable hemostasis,less operation time,minimally invasive and less hospitalization time.Interventional treatment had good effects on blunt liver trauma which were AAST grade Ⅱ to Ⅲ.

Objective:To evaluate the clinical therapeutic effect of L-glutamine granules on intestinal damage of severe burn patients and the safety of the drug.Methods:Twenty-two severe burn patients were randomly divided into control group(C group, n =11) and L-glutamine treatment group ( n =11).GLN treatment group were given L-glutamine in a dose of 30g per day for 7 days and C group were given equal amount of placebo.The plasma L-glutamine concentration,the severity of intestinal mucosa damage, the level of plasma protein and parameters of immunological function were observed and the length of hospital stay was recorded.Results:After 7 days of taking L-glutamine orally,plasma L-glutamine concentration was significantly higher and the severity of intestinal mucosal damage were slighter in GLN treatment group than those in C group.The level of plasma protein and parameters of immunological function were higher in GLN treatment group than those in C group.In addition,the length of hospital stay was shorter in GLN treatment group than those in C group.Conclusion:Administration of L-glutamine can raise the plasma GLN level,abate the severity of intestinal damage obviously, maintain the barrier function of intestinal mucosa, enhance the immunological function, correct the negative nitrogen balance and promote the wound healing with no complications.

Objective:To observe the repairing effects and adverse action of bFGF polypeptide dressings to the superficial second degree burn wounds.Methods:30 superficial second degree burn patients were enrolled in this study.In a self-control study,the trial wounds(A) were applied with polypeptide dressings while the control wounds(B) with the saline gauze dressings to observe the promoting effect of bFGF on the wound healing and the possible adverse action.Results:The average healing time of the trial wounds was 12 days and was 3 days shorter than that of the control wounds.In comparison with control wounds,exudate and local inflammation were slighter on the trial wounds.The effective rate of bFGF polypeptide dressings reached 100%.Conclusion:bFGF polypeptide dressings can promote the healing of superficial second degeree burn wounds and shorten the healing time.This dressing is convenient,safe,non-irritative and has no obvious adverse actions.

Objective:To explore changes of tumor necrosis factor alpha (TNF-?),interleukin-6 (IL-6),interleukin-8 (IL-8) in plasma and the effect of propofol on these cytokines in rats following cerebral ischemia-reperfusion injury.Methods:Focal cerebral ischemia model was induced by occluding the middle cerebral artery (MCA).Seventy two adult Wistar rats were divided randomly into three groups:sham operation group (group A),control group ( group B),which was intraperitoneally infused with 0.9% saline (10ml?kg -1 ) before ischemia and subdiuided into four groups two hours after ischemia and reperfused respectively for one hour,six hours,twelve hours,and twenty-four hours.Propofol group (group C),which was intraperitoneally infused with propofol (100mg.kg -1 ) before ischemia and reperfusion.The serum concentration of TNF-?,IL-6,IL-8 and malondialdehyde (MDA) contents and activities of superoxide dismutase (SOD) were measured.Hematoxylin and eosin staining for the cerebral tissue was processed.Results:The serum concentration of TNF-?,IL-6,IL-8 and MDA of propofol group were significantly lower than control group,while the activity of SOD in plasma was higher than control group.Under light microscope,the structure of cells remained perfect in propofol group,while the cells of control group showed swelling,karyorrhexis and karyolysis.Conclusion:In addition to antioxidation,propofol effectively suppresses the production and release of inflammatory cytokine,and therefore protect the brain from ischemia and reperfusion injury.

Objective: To determine the value of CT,DSA,endoscopic ultra sonography(EUS),and a combined protocol for preoperative detection of insulinomas.Methods : We retrospectively studied data from 15 patients with insulinomas who underwent EUS,CT and DSA from 2000 to 2005.All patients were treated surgically.Results: The overall diagnostic sensitivity for helical CT was 36.3%.Endoscopic sonography and DSA showed sensitivity were 72.7% and 80.0% respectively.The overall diagnostic sensitivity for combined helical CT and endoscopic sonography was 72.7%,which was not statistically significant.The sensitivity for combined EUS and DSA was 93.3%,which was statistically significant.Conclusion: EUS is important to detect insulinomas,and the most effective method for revealing insulinomas is a combined imaging protocol that consists of both EUS and DSA.

Objective:To clone and sequence of 16S~23S r RNA intergenic spacer region of Proteus mirabilis and Acinetobacter lowffii for further identification of these two bacteria by means of molecular probe.Methods: The PCR universal clinical common pathogenic bacteria primers for 16S and 23S rRNA's conserved sequences were designed.Then the genomes of these two bacteria were amplified,T Vector clones were constructed with PMD-18 T Vector ad PCR products.The vectors were sequenced and the sequence was submitted to Genbank.Results: T Vector clones were constructed with products amplified by using universal primers;according to Blast analyses,the results of sequencing were determined as ISR sequences of the two kinds of bacteria.And the reports to Genbank were accepted.Conclusion: Genbank accepted the cloning and sequencing results of Proteus mirabilis and Acinetobacter lwoffii as new sequences.And these sequences can be used to identify the two bacteria by universal primers.

Objective:To construct the Resuscitation-promoting factor B(RpfB)expression plasmid of Mycobacterium tuberculosis, and express and purify it in E.coli.Methods:RpfB gene of Mycobacterium tuberculosis was amplified with PCR method from the H37Rv,cloned into pET32a(+)vector,and then transformed into E.coli Top10.After identifying by restriction digestion and DNA sequencing,the constructed recombinant plasmid was transformed into E.coli BL21,and expressed the recombinant protein under the control initiated by T7 after induction with IPTG.The target protein was identified by Western-blot and purified by using affinity chromatograghy.Results:The size of the RpfB gene digestedby restricted enzymes accorded with the theoretical size and the DNA sequence with the reported one in literatures.61KDa protein was found by SDS-PAGE and Western-blot.Conclusion:The recombinant expression plasmid pET32a(+)-RpfBv of RpfB gene was successfully cloned and constructed,and the recombinant protein with high purity was obteined;which laid the basis for further study.