Objective To investigate the efficacy of treatment with a single loading dose of rosuvastatin before percutaneous coronary intervention (PCI) for prevention of complications after surgery in elderly patients underwent PCI with coronary artery disease.Methods 216 elderly patients with coronary artery disease admitted to Department of Cardiology of Tianjin First Center Hospital were divided into two groups at random (108 patients per group). Within 24 hours before PCI, the patients of loading dose group received a loading dose of rosuvastatin 20 mg, while the patients of regular dose group received a regular dose of rosuvastatin 5 mg, and after surgery, both groups received rosuvastatin 5 mg/d orally. The serum creatinine (SCr), endogenous creatinine clearance rate (CCr), cardiac Troponin I (cTnI), creatine kinase-MB isoenzyme (CK-MB) and hypersensitive C-reactive protein (hs-CRP) before and after PCI were detected, and the incidences of contrast induced nephropathy (CIN) and myocardial infarction were calculated in both groups. The patients were followed up for 1 year after PCI, and the incidence of major adverse cardiac events (MACE) was observed.Results There were no statistically significant differences in the levels of SCr, CCr, cTnI, CK-MB or hs-CRP before PCI between two groups (allP > 0.05). While after PCI in two groups, the levels of SCr, cTnI, CK-MB and hs-CRP were higher than those before PCI, but CCr was lower than that before PCI, and the changes in regular dose group were more significant as compared with loading dose group [SCr (μmol/L): 89.52±21.79 vs. 83.45±19.17, cTnI (μg/L): 0.49±0.23 vs. 0.35±0.18, CK-MB (μg/L): 5.98±1.16 vs. 3.05±0.71, hs-CRP (mg/L): 8.31±0.06 vs. 2.46±0.02, CCr (mL/min): 62.61±19.54 vs. 73.48±20.14, allP < 0.05]. The incidence of CIN [7.41% (8/108) vs. 17.59 (19/108)] and myocardial infarction [1.85% (2/108) vs. 8.33% (9/108)] in loading dose group was evidently lower than that in regular dose group (bothP < 0.05). The incidence of MACE within 30 days after PCI in loading dose group was lower than that in regular dose group [5.56% (6/108) vs. 13.89% (15/108),P < 0.05], while within 1 year after PCI, the comparison of MACE incidence between loading dose group and regular dose group showed no statistically significant difference [19.44% (21/108) vs. 27.78% (30/108),P > 0.05].Conclusion A single loading dose of rosuvastatin given before PCI in elderly patients with coronary cardiac disease can protect renal function and myocardium, reduce the incidences of CIN, myocardial infarction and the occurrence of MACE at early stage after PCI.

Bacteria ; genetics ; pathogenicity ; Dental Research ; Genomic Islands ; Periodontitis ; microbiology

BACKGROUND:An increase in activity of muscle contraction can induce transcription of a variety of signaling molecules to activate a large number of gene expression within the nucleus through proprietary signaling pathway OBJECTIVE:To review studies related the peroxisome proliferator-activated receptor-gamma coactivator 1(PGC-1)and exercise induced skeletal muscle adaptations.METHODS:A computer-based online search of Pubmed was performed for relevant English articles published from January 1995 to January 2009 with the keywords of"PGC1,skeletal muscle,exercise,adaptations"The relevant articles about PGC-1 and exercise induced skeletaI muscle adaptations were included.and repetitive contents were excluded.PGC1 and mitochondrial oxidative metabolism,and exercise-induced skeletal muscle adaptation and PGC1s changed served as the evaluation indicators.RESULTS AND CONCLUSION:Of 57 collected articles,34 were classified and sorted according to the criteria.Endurance training results in profound adaptations for skeletal muscle,including mitochondrial biogenesis,capillary density,and fiber composition.Transcription factors are highly dependent on coactivator molecules to regulate training physiologic adaptation processes.The majority of transcription factors target genes are involved in mitochondrial biogenesis and metabolism.These transcriptional patterns may provide a basic framework for understanding the integration of mitochondrial biogenesis and function with signaling events that exercise induced energetic properties Post-transcription of PGC-1 alpha protein,there are a variety of protein modifications,with a variety of biological processes closely related to the possible adaptive mechanism of exercise-induced skeletal muscle.

OBJECTIVE:To establish a method for the contents determination of cholic acid,hyodeoxycholic acid and deoxy-cholate acid in Naoxueshuan tablet. METHODS:HPLC-ELSD was performed on the column of SHIMADZU VP-ODS C18 with mo-bile phase of acetonitrile-0.2% formic acid(gradient elution)column temperature was 30 ℃,at flow rate of 1.0 ml/min,and the vol-ume injection was 10 μl. RESULTS:The linear range was 0.039 84-0.398 4 mg for cholic acid,0.032 064-0.320 64 mg for hyode-oxycholic acid and 0.0339 52-0.339 52 μg for deoxycholate acid(r≥0.999 0),and the logarithm value of volume injection and peak area showed good linear relationship;RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 101.33%-104.05%(RSD=0.95%,n=6),95.56%-101.38%(RSD=2.54%,n=6)and 96.07%-97.12%(RSD=0.44%,n=6),re-spectively. CONCLUSIONS:The method is simple and accurate,and suitable for the contents determination of cholic acid,hyode-oxycholic acid and deoxycholate acid in Naoxueshuan tablet.

Aiming at the shortcomings of the traditional nuclear medicine teaching model and attempting to adapt to the needs of the development of clinical nuclear medicine,we changed teaching methods,strengthened practice teaching,performed online learning,introduced the self-made teaching materials,enriched the teaching contents,proposed the concept of comparative imaging and reformed on examination method.These measures greatly improved students' interests in learning and clinical thinking ability and helped students understand and master the theoretical knowledge of medicine.

Objective To construct a lentiviral expression vector of murine RelB for RNAi,and then to effectively silence the RelB gene expression of murine bone marrow derived dendritic cells for constructing the bone marrow tolerogenic dendritic cell,in order to provide an experimental foundation for a novel clinical therapy of autoimmune disease.Methods RelB shRNA sequence of mouse was designed by on-line designer software on Corp.Invitrogen,after synthesis and annealing,double strand oligonucleotides(dsoligoes)were cloned into the pENTRTM/U6 plasmid,then after sequencing,a positive clone was further subcloned into pLenti6/BLOCK-iTTM-DEST vector.It was then transformed into stb13 competent cell,and after sequencing,293FT cell line was transfected by above positive recombined plasmid and lentiviral packing materials.It was incubated for 48 to 72 h in a 37℃,5% CO2 incubator.Culture supernatant was harvested and stored at-80℃,then the virus titer was determined by serial dilution assay.Results It was showed from sequencing figures that all the pENTRTM/U6-RelB-shRNA plasmids were positive clone vector,and this positive recombinant vector was recombined with pLenti6/BLOCK-iTTM/U6-DEST vector.It was then transformed into stb13 competent cell and screen positive clone by ampicillin,and resequenced by the use of primer forward U6 primer.The results also showed that recombinant lentiviral vector was positive clone.Viral particle was packaged with other packaging material mediated by lipofectamine 2000 in 293FT cell line.Cultural supernatant was collected and stored at-80℃,and lentiviral particle titer was determined by serial dilution assay with 6?105/transduced unit.Conclusion Lentiviral shRNA expression vector of murine RelB gene for RNAi was successfully constructed.It might be a rational procedure to make tolerogenic DC,and then to develop a DC vaccine and DC-based immunotherapy for autoimmune diseases.

Objective To evaluate the value of power Doppler and contrast-enhanced ultrasonography in the detection of tumor after microwave tissue coagulation (MTC). Methods Ultrasound Microwave Theraphy-1 (UMT-1) equipment was used,and a 9 mm length antenna was inserted directly into the tumor at laparotomy in 10 VX 2-bearing rabbits. The energy output was 60 W?120 s per bout. Two-dimensional, power and contrast-enhanced (Levovist, 300 mg/ml, 0.3 ml/kg) imaging was performed pre- and post-treatment. Pathology examination was applied in contrast. Results Along the needle being narrow hyper echoic imaging, broad-equable hypo echoic imaging was observed around the center after MTC immediately and the edge of tumor was unclear. Intratumoral blood flow was observed in 4 VX 2 tumors with power ultrasonography while there was enhanced-signal in 10 VX 2 tumors using contrast-enhanced ultrasonography. These tumors were confirmed incomplete necrosis by pathology. Conclusions Contrast-enhanced power Doppler ultrasonography shows more sensitive in assessing the therapeutic effect of microwave on VX 2 tumor. Residual tumor can be seen as persistent color enhancement by using contrast-enhanced ultrasonography.

Objective To compare the effectiveness of acetic acid injection(AI) and microwave(MW) coagulation in the treatment of rabbits hepatic VX 2 tumors. Methods Eighteen rabbits with VX 2 tumor were divided into two groups randomly,one was treated by precutaneous acetic acid injection,and the other by microwave at laparotomy. Contrast-enhanced ultrasonography (Levovist) was used to assess the therapeutic effect after treatment. Pathologic changes were observed chronologically. Results Anomalo-necrosis areas including tumors and surrounding normal liver tissue were observed after AI,multiple point or sheet necrosis was confirmed by pathology. Long oval-shap coagulated areas were observed after MW,incomplete necrosis after treatment was immediately confirmed by pathology and changed to complete necrosis in two weeks. Conclusions AI is more effective in the treatment of liver tumors,but diffuses easily to normal liver and the necrosis area is not stable. MW thermal field is steady and necrosis area is reliable.

Objective To evaluate the differentiation diagnostic value of acoustic radiation force impulse imaging (ARFI) technique to the benign and malignant breast lesions .Methods A total of 87 breast masses in 84 patients were selected and per‐formed the routine ultrasound ,virtual touch imaging(VTI) and virtual touch quantification(VTQ) examination .The shear wave ve‐locity was obtained .The average optical density of the tumor was obtained by processing the VTI images by using the image J soft‐ware .The pathological results served as the gold standard and the ROC curve was drawn .Finally ,the significance of ARFI was e‐valuated .Results All 87 pathologically verified breast tumors included 31 malignant lesions and 56 benign lesions .In malignant le‐sions ,there were 21 lesions with VTQ value≥3 .49(threshold value) and 10 lesions with VTQ value<3 .49 .In benign lesions ,there were 3 lesions with VTQ value≥3 .49 and 53 lesions with VTQ value<3 .49 .In malignant lesions ,there were 28 lesions with the mean optical density value≥202 .25(threshold value) and 3 lesions with the mean optical density value<202 .25 .In benign lesions , there were 6 lesions with the mean optical density value≥202 .25 and 50 lesions with the mean optical density value<202 .25 .There were statistically significant differences in the shear wave velocity measured by VTQ and average optical density measured by VTI between malignant and benign lesions(P=0 .000) .The sensitivity ,specificity ,positive predictive value ,negative predictive value ,ac‐curacy rate and Youden index of conventional ultrasound were 96 .8% ,67 .8% ,62 .5% ,97 .4% ,78 .2% ,0 .645 ,respectively ;which for ARFI were 93 .5% ,98 .2% ,96 .7% ,96 .5% ,96 .6% and 0 .917 ,respectively .Conclusion The ARFI is superior to the conven‐tional ultrasound in the specificity and accuracy rate in diagnosing breast tumor .

Objective To study the effect of morin on LPS induced acute lung injury mouse model and its mechanism .Meth‐ods Thirty male C57B/L mice were randomly divided into control group ,LPS group and LPS+ morin group ,with 10 in each group .5 mg/kg LPS was instilled into the lung from an trachea intubation in LPS group and LPS+morin group .Then the mice in LPS+morin group received an intraperitoneal injection of morin (40 mg/kg) every day for the next 3 d .Others received an equal a‐mount of saline .After 72 h ,the mice were sacrificed .The bronchoalveolar lavage fluid (BALF) was collected and centrifuged;the sediments were stained with Wright‐Giemsa for total cell and neutrophil count and the supernates were prepared for ELISA .The wet and dry weight of lung was weighed to calculate the wet/dry weight ratio .HE staining was performed to examine the pathologi‐cal change of lung .Western blot was used to determined the expression of TLR4 ,IKK and NF‐κB .Results Intratracheal instillation of LPS successfully established ALI model in mouse .LPS caused significant pathological changes including inflammatory cells infil‐tration ,alveolar septa thickness ,hemorrhage and edema .The wet/dry weight ratio ,the total cell count ,neutrophil count ,TNF and IL‐1βlevel in BALF ,and the expression of TLR4 ,NF‐κB ,and IKK were all increased significantly (P<0 .05) ,which were allevia‐ted by intraperitoneal injection of morin .Conclusion Morin can dampen the inflammatory response during LPS induced ALI in mouse ,which is potentially attributed to its inhibitory effect on the activation of NF‐κB .