MicroRNA (miRNA) regulate the expression of target mRNA according to the specific base pairing. They are involved in proliferation,differentiation and apoptosis of cells. The expression of miRNA has distinct differences between normal tissues and cancers, miRNA can be oncogenes but also suppressor genes, they play an important role in cancer. And miRNA are promising to be a new therapeutic target. In this review, we will discuss the advance in the research of miRNA in cancer.

BACKGROUND: Cecropins are a kind of micromolecule protein with antibacterial activity. Eukaryotic cell-expressed or artificially synthesized Cecropins is characterized by low efficiency and high cost. OBJECTIVE: To clone and express an antibacterial peptide gene of Musca domestica Cecropin A, and to identify recombinant expression product. METHODS: Mature Musca domestica Cecropin A encoding nucleotide sequence was searched from the GenBank and amplified by RT-PCR. The gene of Musca domestica Cecropin A was cloned into prokaryotic expression vector pET32a and fused with gene of Thioredoxin (Trx) and expressed in E.coli BL2l (DE3). After induction by isopropyl-β-D-thiogalactoside, the sera of the immunized rabbits were collected after rabbits were immunized with the hemolymph of housefly larvae. Recombinant protein was identified by western blot analysis and N-[Tris(hydroxymethyl)methyl]glycine-sodium dodecylsulfate-polyacrylamide gel electrophoresis.RESULTS AND CONCLUSION: After induction by isopropyl-β-D-thiogalactoside, E.coli BL21 expressed mature Cecropin. Rabbit anti- housefly larvae sera, N-[Tris(hydroxymethyl)methyl]glycine-sodium dodecylsulfate-polyacrylamide gel electrophoresis and western blot analysis results confirmed that expression products were mature Cecropin. These suggest that prokaryotic expression system can be utilized to obtain natural mature Cecropin.

Objective: To observe the clinical effects of needling acupoints Shenting(GV 24),Neiguan(PC 6) and Sanyinjiao(SP 6) in the treatment of migraine. Methods: Thirty cases of Germen patients with migraine were treated mainly by Shenting(GV 24), bilateral Neiguan(PC 6) and Sanyinjiao(SP 6), as well as bilateral Hegu(LI 4) and Taichong(LR 3), Touwei(ST 8),Yangbai(GB 14), Cuanzhu(BL 2), Sizhukong(TE 23), Shuaigu(GB 8) and Sibai(ST 2) in the affected side of head, auricular Sympathetic (MA-AH 7), Er Shenmen (MA-TF 1), Heart (MA-IC) and Endocrine (MA-IC 3); another 28 cases were treated by routine acupuncture. The clinical results and immediate pain-relieving effects were observed. Results: The total effective rate was 90.0% in treatment group and 75.0% in control group, χ2= 4.57, P ＜ 0.05; the 24-hour pain-relieving effects were obviously better in treatment group than that in control group, χ2 = 10.11, P＜ 0.01. Conclusion: Needling Shenting(GV 24), Neiguan (PC 6) and Sanyinjiao(SP 6) has better effects than routine acupuncture in the treatment of migraine.

Objective To understand the freshmen’s status of hepatitis B virus infection,immunological effect after vaccina-tion against hepatitis B from a university in Ankang city,to provide a scientific basis for university’s prevention from hepati-tis B.Methods Analyzed the results of five markers of hepatitis B virus infection and alanine aminotransferase (ALT)of 25 895 freshmen in a college in Ankang for resent 12 years,and observed the HBsAb serocoversion in serum 1 year after vacci-nation against hepatitis B and after strengthened immunity.Results The HBeAg positive rate of the freshmen was 4.07%, among which the male was 5.58% (281/5 035),and the female was 3.14% (258/8 214).The difference had significance of statistic (P<0.05).The college students’HBeAg positive rate from rural area and from urban area were 4.77% and 2.93%respectively,the difference had significance of statistic (P<0.05).There was no significant statistic difference in immune effect of hepatitis B vaccine for different sex (χ2=5.80,P<0.05).Their HBsAb positive rate was 95.15% after injected with 10μg hepatitis B and 5μg hepatitis B vaccine,which had significance of statistic (P<0.05).Conclusion The hepatitis B virus infection rate and the hepatitis B protective antibody of the college freshmen in Ankang district was lower than that of the whole country and had a decreased tendency.It showed the male’s infection rate of hepatitis B virus was higher than the female,the rural was higher than the urban,and the immune effect of hepatitis B vaccine proved to be good.It should be-strengthened immunity for non-responders to ensure the immune effect if necessary.

Objective To clarify the morphological features,types,regional distributions and cell densities of gastrointestinal endocrine cells in various parts of the digestive tracts of Canis lupus and Canis familiaris. Methods Immunohistochemical techniques of streptavidin-peroxidase(SP) method was used. Results Seven kinds of gastrointestinal endocrine cells were found positive in the digestive tracts of Canis lupus and Canis familiaris.The endocrine cells were mainly located between the epithelia cells of gastric gland,intestinal villus and intestinal gland.There were also some endocrine cells distributed in the lamina propria and submucosa.The endocrine cells found in the digestive tract were in various shapes.Most of the endocrine cells had long cell processes run ning out to the lumen or the nearby cells.Among these endocrine cells,5-hydroxytryptamine(5-HT) positive cells had a widest distribution,followed by vasointestinal poplypetide(VIP) positive cells.The distributions of other cells were limited.From the comparison of the characters of endocrine cells of Canis lupus and Canis familiaris,most of the endocrine cells had similar morphological features and regional distributions except pancreatic polypeptide(PP) positive cells and substance P(SP) positive cells.There were differences in cell densities of some endocrine cells.Conclusion The results of Canis lupus and Canis familiaris reflect the similarity of mammals in the characteristics of the morphological features,types,regional distributions and cell densities of gastrointestinal endocrine cells,as well as the similarity in digestive physiology of canine animals.At the same time,the results of Canis lupus also exhibit characteristics of gastrointestinal endocrine cells of wild animals.

0.01), but the significant difference was found respectively between group C and B and between group C and A (P

Objective To study the effects of violet flower Bidai extracts on body weight,blood lipid level,serum leptin level,the activity of lipoprotein lipase and hepatic lipase of obese rats,and to discuss the mechanism of violet flower Bidai extracts on weight reduction.Methods Male obese rats which induced by high energy diet were randomly divided into four groups.All rats were given high energy diet.One control group was gavaged with normal saline and one western medicine group was gavaged with sibutramine,and two violet flower Bidai extracts groups was gavaged with water solutions of violet flower Bidai extracts at doses of 6 g(crude drug)/kg BW,12 g(crude drug)/kg BW,respectively.After six weeks,body weight,obese index,weight of abdominal fat tissue,fasting lipid,leptin in serum,the activity of lipoprotein lipase and hepatic lipase were measured.Results Body weight,obese index,weight of abdominal fat tissue in the two violet flower Bidai extracts groups were significantly lower than those in obese control group(P

0.05).The splicing destruction among each group was the main breakage method between materials and fiber posts.The surface treatment of fiber posts with sandblast or hydrogen peroxide significantly enhanced the bond strength of the composite resin tested.The surface treatment of fiber posts with silane did not enhance the bond strength of the composite resin.

Imitating the structure of coupled biological nervous system, artificial neural network (ANN) can be applied to pattern analysis, signal procession and etc. This paper mainly explains physical concepts to make the medical students and workers familiar with the neural network, especially ANN and its applications to the detection and analysis of medical image.

Statins may not only lower cholesterol concentration,reduce the incidence of heart diseases and relieve kidney damage,but also stimulate bone formation obviously,as suggested by a number of observation studies and animal researches.However,the exact mechanism of promoting bone formation has not been very clearly yet.Moreover,their adverse effects and their interaction with other drugs still need further clinical trials.In this review,on the basis of clinical data about the effects of hydroxymethylglutaryl-CoA(HMG-CoA)reductase inhibitors,we summarize current hypotheses on their mechanism and safety,in order to provide reference for the domestic bone reconstruction research.