From laboratory Physicians's localization to discuss the clinical practice teaching of undergraduate student,to cultivate the ability of turning laboratory data into effective information;to strengthen the teaching of evidence based laboratory medicine,to participate in clinical pathology discuss and to combine clinic to make scientific researches.It is very important to cooperate with the clinic in the practicing teaching.

Objective To investigate the effect of methylprednisolone pulse therapy on spinal nerve functions in patients with acute spinal cord injury (ASCI) and evaluate its safety. Methods 200 patients with ASCI treated in our hospital from January 2010 to December 2016 were selected and were randomly divided into two groups,with 100 cases each group. The patients in control group were treated with routine basic treatment while patients in the study group were treated with methylprednisolone pulse therapy. On the basis of the American spinal cord injury Society scoring criteria (ASIA), the neurological functional recovery scores were performed were scored before and 6 weeks after treatment between the two groups and the adverse reactions were recorded. Results The scores of sensory and motor function of the patients in the two groups were higher than those before the treatment and there was statistical difference (P<0.05), and the scores in the study group was significantly higher than those in the control group, and the difference between the two grouPs was statistically significant (P<0.05). The incidence of adverse reactions in the study group (25.00％) was significantly higher than that in the control group (10.00％), and the difference was statistically significant (P<0.05). The incidence of lung infection and gastrointestinal reaction in the study group were significantly higher than those in the control group and there were statistical difference (P<0.05). Conclusion Methylprednisolone pulse therapy has significant effect on the recovery of neurological function in patients with ASCI, but the incidence of adverse reactions is high, so it is necessary to strengthen the monitoring and intervention in clinic.

0.05). Conclusion The movement of train has not obviously affect to the routine blood test.

Uveitis is a kind of inflammatory disease affected in ocular posterior segment.Uveitis sustains a long duration and causes a significant damage of visual function.It often leads to serious complications,such as cystoid macular edema (CME),cataract,glaucoma,retinal detachment and choroidal neovascularization (CNV).In the pathological process of uveitis,inflammatory factors promote the release of vascular endothelial growth factor (VEGF) through a variety of pathways.It has been verified that VEGF level is elevated in vitreous in the patients with uveiticinduced CME.Intravitreal injection of anti-VEGF drugs,including ranibizumab and bevacizumab,can improve the visual acuity in most patients with noninfectious uveitic-induced CME and CNV.However,intravitreously repeated injections of anti-VEGF drugs may be required in some patients.Intraocular injection of anti-VEGF drugs is an optional and effective way for those with persist macular edema and not being suitable for the use of corticosteroid or immunosuppressive therapy.However,anti-VEGF drugs may affect the efficacy of corticosteroid.Therefore,more clinical and basic researches are still needed.The status and progresses in the use of anti-VEGF drugs for uveitisinduced CME and CNV were reviewed.

Objective The aim of our study was to evaluate the efficacy and safety of tirofiban in patients with acute coronary syndromes with thrombus-containing lesions.Methods We randomized 38 acute coronary syndromes(ACS)patients with thrombus-containing lesions into tirofiban group and control group.In the tirofiban group,10～30 mL of tirofiban was administered intracoronar before and/or after percutanous transluminal coronary angioplasty(PTCA),then stent was implanted as needed.Intravenous tirofiban was used for 24～48 hours after the procedure.In the control group,standard percutanous coronary intervention(PCI)was used for the patients.TIMI thrombosis grade,TIMI blood flow,no reflow and/or slow flow,corrected TIMI frame count(cTFC),myocardial blush grade(MBG),left ventricular eject fraction(LVEF)were measured in the two-dimensions untrasonography;major adverse cardiac events(MACE)and bleeding were observed.Results TIMI thrombosis grade and cTFC decreased more in the tirofiban group than that of the control group[0.90?0.14 vs 1.53?0.54,P

The number of projects from free applications of the national natural science foundation of China (NSFC) about pharmacology of antitumor drugs in 2001 increased evidently than before. Those projects were mainly about the exploration of the natural materials with antitumor activity, investigation in new targets of antitumor drugs, study in the inhibition of angiogenesis in tumor cells,etc. The tumor pharmacology in China has made some progress, but it still lags much behind the international study level. Basic work and creative ideas should be strengthened for further development.

Objective To optimize the extraction conditions of Hyperici Perforate Herba, Coptidis Rhizoma, Schisandrae Chinensis Fructus, and Ziziphi Spinosae Semen forJinzao Yuanzhi Capsule.Methods Hyperin extraction rate was set as index, and HPLC was used for determination. The extraction conditions of ethanol concentration, ethanol quantity, extraction time and other factors were studied with orthogonal test and comparative test.Results Ethanol concentration and extracting times were remarkable factors in the test. The optimum extraction technology forJinzao Yuanzhi Capsule was to reflux and extract the materials with 8 times of 70% ethanol for 2 times, reflux extraction for 1.5 hours, filtration. Then 6 times of 70% ethanol was added for 1.5 hours’ reflux, filtering, and merging extracted liquid.Conclusion The optimum extraction technology is reasonable and feasible.

Objective To investigate the clinical manifestations and pathogen distribution of the neonatal sepsis, and to analyze the antibiotic resistance of the pathogens. Methods Review the Medical records of 38 sepsis cases of full-term and premature neonates in our hospital from October 2011 to February 2014 were col-lected and analyzed. Results Ten cases were caused by Gram-positive bacteria among the 18 full-term neonates with sepsis. Eight of ten of the isolates were resistant to oxcillin. Nine of ten of the cases were belonged to late onset infection, and the cases with no nosocomial infection were found. In the other eight full-term neonatal cases caused by Gram-negative bacteria , six cases were nosocomial infection. Among the 20 premature neonates with sepsis , 17 cases were infected Gram-negative bacteria , in which Escherichia coli , Klebsiella pneumonia and En-terobacter cloacae were the most common agents (16/17). Early onset type and nosocomial infection were identi-fied for 11 (11/20) and 9 (9/20) cases in the premature neonates, respectively. The penicillin G, methicillin resistant rates of the Gram-positive bacteria were close to or over 70%. All the Gram-positive bacteria were sensi-tive to vancomycin. All the Gram-negative bacteria were resistant to amoxicillin , but over 60% of them were sen-sitive to piperacillin-tazobactam and other compounds containing enzyme inhibitor , and 100% of them were sensi-tive to carbapenems and aminoglycoside. Conclusion The full-term neonatal sepsis admitted into our hospital were mainly caused by Gram-positive bacteria , which were usually resistant to oxcillin. The premature sepsis were mainly caused by Gram-negative bacteria , which were always sensitive to carbapenems and aminoglycoside.

BACKGROUND: Several studies have demonstrated that the range,thickness and morphology of oral prosthesis directly influence patient's phonetic function,and titanium base and polymethyl methacrylate(PMMA)resin base yield different influences on phonetic function due to different thicknesses of materials.OBJECTIVE: To analyze the phonetic parameters in one complete denture patient who wear titanium base and PMMA resin base simultaneously and investigate the influences of maxillary complete denture base on phonetic function.METHODS: Through the use of computer-based voice analysis software PRAAT4.4.34,frequency value of the first concentrated frequency area when speaking consonant sounds/x,sh,r,zh,ch,j,q/and voice onset time when speaking consonant sounds/g,k,zh,ch,j,q/were statistically analyzed in the same patient who separately wear titanium complete denture base and PPMA resin complete denture base,and spectrogram of velar stops between complete denture bases was compared.RESULTS AND CONCLUSION: MMPA resin complete denture base was to some extent poor in promoting the recovery of phonetic function owing to great base thickness.Titanium complete denture base with less thickness in the tongue or palate region would help recovery of phonetic function.Extraction and analysis of phonological parameters,such as frequency value of first concentrated frequency area and voice onset time,using CSL computer*based voice analysis system,would provide objective evidence for lamprophony analysis after wearing complete denture.Maxillary denture with thinner base in tongue and palate regions better promotes the recovery of patient's phonetic function.

Objective:Tumor suppressor gene nitrogen permease regulator like 2 (NPRL2), also called tumor suppressor candidate gene 4 (TUSC4), widely exists in all kinds of human tissues and is highly conserved in different kinds of biological species. The expression of NPRL2 is markedly decreased in many human tumor tissues, such as lung cancer, breast cancer,renal carcinoma, et al. NPRL2 is located in human chromosome 3p2.13, and participates in DNA mismatch modification, regulation of signal transduction du-ring cell cycle progression, and apoptosis induction. NPRL2 gene inactivation is related with the initiation and progression of several malignant tumors. The mechanism underlying the tumor-inhibiting activity of NPRL2 gene is not clear and needs to be further investigated.