Objective To analyze the clinical characteristics and drug resistance of gram -negative bacillus in Department of Respiratory Medicine.Methods 100 patients in Department of Respiratory Medicine were selected, analyzed the clinical characteristic and drug resistance of 125 strains of gram -negative bacillus that was separated. Results 125 strains of pathogenic gram negative bacilli were isolated out of 100 patients,among which 34 strains were pneumonia klebsiella bacteria,24 strains were acinetobacter baumannii,18 strains were pseudomonas aeruginosa, 10 strains were enterobacter cloacae,18 strains were escherichia coli and 21 strains were other bacteria.Results The drug resistance rates were 79.4%,76.5%,76.5% and 76.5% respectively in klebsiella pneumoniae to amikacin, cefotaxime,ampicillin and imipenem,was more sensitive to meropenem and cefoperazone,the drug resistance rate was 100.0% in acinetobacter baumannii to ampicillin and cefazolin,and was 91.7% to ceftriaxone,and the resistance rate for other drugs are higher generally.The drug resistance rate was 100.0% in pseudomonas aeruginosa to ampicillin and ceftriaxone,and was 94.4% to cefotaxime and levofloxacin,the drug resistance rate was 90.0% in enterobacter cloacae to ampicillin,and was 60.0% to ceftriaxone,cefazolin and cefoxitin,the drug resistance rate on the other drug was relatively low.Conclusion Klebsiella pneumoniae,acinetobacter baumanni and pseudomonas aeruginosa were the main gram negative bacillis the cause hospitalized patients with drug resistance.

Objective To explore the efficacy of neuromuscular electrical stimulation (NMES) in treating severe and chronic obstructive pulmonary disease (COPD).Methods Seventy-eight COPD patients were randomly divided into an NMES group of 38 and an exercise therapy group of 32.In addition to the routine drug therapy and basic rehabilitation training,the NMES group received 8 weeks of NMES of the lower limbs while the exercise group was provided with strength and endurance training of the lower limbs,also for 8 wceks.Before and after the 8 weeks of treatment,pulmonary function was tested along with isokinetic quadriceps strength,cardiopulnonary capacity,and lung capacity using Saint George's respiratory questionnaire (SGRQ).Results The two groups showed no significant difference in any of the measurements before the treatment.After the treatment,forced expiratory volume in 1 second of (FEV1),FEV1/forced vital capacity (FVC),FEVI in percentage of the predicted value,medical research council dyspnea scale (MRC) score and the 6-minute walk distance (6MWD) improved in both groups.However,the average body mass index and 6 minute walk distance differed significantly between the groups after the treatment.The quadriceps' peak torque increased after the treatment in both groups,but the average in the exercise group was significantly higher than that in the NMES group.VO2max and Wmax also increased significantly in both groups,and the average time to exhaustion increased significantly in the exercise group.Significant improvement was observed in the BODE assessment after the intervention for both groups.Conclusion Neuromuscular electrical stimulation is as effective as therapeutic exercise in promoting the rehabilitation of severe COPD patients.However,lower limbs exercise is still the first choice in managing such patients.