Objective To investigate the effects of the expressions of thymidine kinase 1 (TK1) and Ki67 alone or their combination on the recurrence and metastasis of breast cancer.Methods Sixty-five samples were selected from the Second Affiliated Hospital of Guangzhou Medical University from March 2005 to June 2007,which were resected by surgical operation and confirmed as breast carcinoma by pathology.They were individed into two groups including 37 cases with recurrence or metastasis in 5 years (group A),28 cases without recurrence or metastasis in 5 years (group B).The expressions of TK1 and Ki67 in the two groups were detected by immunohistochemical staining assay.Then,Kaplan-Meier assay was used to describe survival curve.Results The positive expression rate of TK1 in group A was 91.8％,which was dramatically higher than that in group B 67.8％ (x2 =6.116,P =0.013).The positive expression rates of Ki67 in group A and B were 78.4％ and 42.9％ (x2 =8.635,P =0.003).The positive expression rates of TK1 combined with Ki67 in group A and B were 67.6％ and 39.3％ (x2 =5.159,P =0.023).Moreover,disease free survival of patients with positive expression of TK1 combined with Ki67 decreased significantly,compared with patients with positive expression of TK1 or Ki67 alone (x2 =6.137,P =0.046).Conclusion Positive expression of TK1 combined with Ki67 is the high risk factor of the reccurence or metastasis of breast carcinoma,and indicates poorer prognosis compared with positive expression alone.

Objective:To investigate the therapeutic effects of different frequencies of repetitive transcranial magnetic stimulation (rTMS) on first-episode schizophrenia with negative symptoms, providing evidence for diagnosis and treatment of this disease.Methods:The clinical data of 84 patients with first-episode schizophrenia with negative symptoms who received treatment in the Second People's Hospital of Lishui from October 2017 to October 2019 were retrospectively analyzed. These patients were divided into 5 Hz, 10 Hz, and 15 Hz groups according to different rTMS frequencies ( n = 28/group). Patients in the three groups received 5, 10, and 15 Hz of rTMS. All patients received eight courses of the 1-week treatment. The therapeutic effect was compared between groups at the end of treatment. The scores of the Positive and Negative Syndrome Scale (PANSS), Scale for Assessment of Negative Symptoms (SANS), Personal and Social Performance Scale (PSP), and The Loewenstein Occupational Therapy Cognitive Assessment (LOTCA) pre- and post-treatment, as well as adverse reactions, were determined in each group. Results:Total response rate in the 10 Hz group was 92.86% (26/28), which was significantly higher than that in the 5 Hz group [71.43% (20/28)] and 15 Hz group [67.86% (19/28), χ2 = 5.54, P = 0.019]. The scores of PANSS and SANS post-treatment in the 10 Hz group were (61.28 ± 4.16) points and (34.17 ± 5.02) points, respectively, which were significantly higher than those in the 5 Hz group [(67.19 ± 3.89) points, (42.98 ± 4.17) points] and 15 Hz group [(67.10 ± 3.94) points and (43.21 ± 3.56) points, F = 20.09, 40.38, all P < 0.001]. The score of PSP post-treatment in the 10 Hz group was (60.13 ± 3.78) points, which was significantly higher than that in the 5 Hz group [(54.13 ± 4.12) points] and 15 Hz group [(53.20 ± 3.79) points, F = 26.05, P < 0.05]. The score of LOTCA in the 10 Hz group was (40.13 ± 4.12) points, which was significantly lower than that in the 5 Hz group [(46.28 ± 4.20) points] and 15 Hz group [(47.13 ± 3.89) points, F = 24.64, P < 0.001). The incidence of adverse reactions in the 5 Hz, 10 Hz, and 15 Hz groups was 17.86% (5/28), 7.14% (2/28), and 14.28% (4/28), respectively. There was no significant difference in the incidence of adverse reactions among the three groups ( χ2 = 1.46, P = 0.481). Conclusion:10 Hz rTMS can effectively improve the negative symptoms, cognitive function, and social function of schizophrenic patients. The therapy is of high clinical value.

Objective:To investigate the efficacy of tandospirone combined with venlafaxine in the treatment of comorbid anxiety and depression and its effects on neurotransmitters and related factors.Methods:A total of 92 patients with comorbid anxiety and depression who received treatment in the Second People's Hospital of Lishui between June 2019 and June 2020 were included in this study. They were randomly divided into an observation group and a control group ( n = 46/group). The control group was treated with venlafaxine, while the observation group was treated with tandospirone and venlafaxine. Before and after treatment, the scores of Hamilton Anxiety Scale (HAMA) and Hamilton Depression Scale (HAMD), the levels of 5-hydroxytryptamine, brain-derived neurotrophic factor, nerve growth factor, and adverse drug reactions were compared between the two groups. Results:At 4 and 8 weeks after treatment, HAMA scores in the observation group were (11.39 ± 3.11) points and (8.26 ± 2.18) points, respectively, which were significantly lower than (14.72 ± 3.57) points and (10.46 ± 2.37) points in the control group ( t = 4.77, 4.63, both P < 0.05). At 4 and 8 weeks after treatment, HAMD scores in the observation group were (15.95 ± 2.90) points and (9.33 ± 1.54) points, respectively, which were significantly lower than (17.43 ± 2.87) points and (13.28 ± 2.65) points in the control group ( t = 2.46, 8.74, both P < 0.05). After treatment, 5-hydroxytryptamine, nerve growth factor, and brain-derived neurotrophic factor levels in the observation group were (154.59 ± 45.26) μg/L, (13.62 ± 1.16) ng/L, (28.54 ± 2.33) ng/L, respectively, which were significantly higher than (129.99 ± 48.31) μg/L, (11.98 ± 1.04) ng/L, and (25.69 ± 2.51) ng/L in the control group ( t = 2.52, 7.14, 5.64, all P < 0.05). There was no significant difference in the incidence of adverse reactions between the two groups ( χ2 = 0.81, P = 0.369). Conclusion:The adjuvant treatment with tandospirone can markedly improve anxiety and depression and protect neurological function of patients with comorbid anxiety and depression, and is highly safe.