1.Effects of Transcranial Direct Current Stimulation on Naming of Visual and Auditory Modality in Post-stroke Aphasia
Yuan-yuan TAO ; Rong SUN ; Jie LE ; Hai-xia MI ; Xiao-xia DU ; Lu-ping SONG
Chinese Journal of Rehabilitation Theory and Practice 2019;25(5):506-512
Objective:To observe the effects of transcranial direct current stimulation (tDCS) on the naming of visual and auditory modality in patients with post-stroke aphasia. Methods:From March to November, 2018, 32 patients with post-stroke aphasia were randomly divided into control group (
2. Establishment and evaluation of a nomogram model of systemic inflammatory response syndrome after percutaneous nephrolithotomy
Fang-ming XU ; Lu BAI ; Sen ZHANG ; Ya-le LU ; Chao MI ; Jie DUAN ; Shu-bin CAO ; Shu-yuan CHEN ; Li GAO
Journal of Medical Postgraduates 2019;32(9):968-972
Objective The main cause of systemic inflammatory response syndrome(SIRS) after percutaneous nephrolithotomy(PCNL) was still unclear. The purpose of this study was to investigate the risk factors associated with SIRS after PCNL and establish the nomogram model. Methods A retrospective analysis of 213 cases of PCNL patients due to upper urinary calculi admitted to urology department in affiliated hospital of guilin medical college from December 2017 to December 2018 was performed. According to the occurrence of SIRS, patients were divided into SIRS group (SIRS patients) and control group (patients without SIRS). Logistic regression was used to analyze the risk factors of SIRS after PCNL, and a nomogram model was established based on logistic regression model. Results There were 54 cases in the SIRS group and 159 in the control group. Gender(OR=2.547, 95%cl:1.229-5.275), diabetes (OR=5.027, 95%cl: 1.442-17.525), calculi surface area (OR=2.657, 95%cl: 1.206-5.853), NLR immediately after surgery (OR=3.793, 95%cl: 1.749-8.02), operation time (OR=2.985, 95%cl: 1.305-6.826), and blood transfusion (OR=12.50, 95%cl: 12.50). 1.954-80.056) were the risk factors of SIRS after PCNL (
3.Mechanism of Proliferation and Apoptosis of Acute Promyelocytic Leukemia Cell Line NB4 Induced by TPA.
Pan ZHAO ; Chong ZHANG ; Xue-Mei DONG ; Lu-Wei YAN ; Le-Yuan MI ; Ya-Jiao LI ; Jia-Chao KANG ; Jing WANG
Journal of Experimental Hematology 2023;31(5):1296-1302
OBJECTIVE:
To investigate the effect of phorbol-12-myristate-13-ace-tate (TPA) on the proliferation and apoptosis of acute promyelocytic leukemia cell line NB4 and its molecular mechanism.
METHODS:
The effect of different concentrations of TPA on the proliferation of NB4 cells at different time points was detected by CCK-8 assay. The morphological changes of NB4 cells were observed by Wright-Giemsa staining. The cell cycle and apoptosis of NB4 cells after TPA treatment were detected by flow cytometry. The mRNA expressions of NB4 cells after TPA treatment were analyzed by high-throughput microarray analysis and real-time quantitative PCR. Western blot was used to detect the protein expression of CDKN1A, CDKN1B, CCND1, MYC, Bax, Bcl-2, c-Caspase 3, c-Caspase 9, PIK3R6, AKT and p-AKT.
RESULTS:
Compared with the control group, TPA could inhibit the proliferation of NB4 cells, induce the cells to become mature granulocyte-monocyte differentiation, and also induce cell G1 phase arrest and apoptosis. Differentially expressed mRNAs were significantly enriched in PI3K/AKT pathway. TPA treatment could increase the mRNA levels of CCND1, CCNA1, and CDKN1A, while decrease the mRNA level of MYC. It could also up-regulate the protein levels of CDKN1A, CDKN1B, CCND1, Bax, c-Caspase 3, c-Caspase 9, and PIK3R6, while down-regulate MYC, Bcl-2, and p-AKT in NB4 cells.
CONCLUSION
TPA induces NB4 cell cycle arrest in G1 phase and promotes its apoptosis by regulating PIK3/AKT signaling pathway.
Humans
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Leukemia, Promyelocytic, Acute
;
Caspase 3/metabolism*
;
Caspase 9/pharmacology*
;
Phosphatidylinositol 3-Kinases/metabolism*
;
Proto-Oncogene Proteins c-akt/metabolism*
;
bcl-2-Associated X Protein/metabolism*
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Cell Line, Tumor
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Cell Division
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Apoptosis
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RNA, Messenger
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Cell Proliferation
4.Effect of Dichloromethane Extraction Phase of Patrinia Scabiosaefolia Fisch. Stem on Proliferation and Differentiation of K562 Cells.
Le-Yuan MI ; Ke-Jing LI ; Shan LI ; Ting LIU ; Xiao-Jing CHAI ; Ying ZHANG ; Juan LI
Journal of Experimental Hematology 2023;31(1):25-32
OBJECTIVE:
To explore the effect of dichloromethane extraction phase of ethanol extract from stem of Patrinia scabiosaefolia Fisch.(DPSS) on proliferation and differentiation of K562 cells and its related mechanism.
METHODS:
MTT assay was used to detect the effects of DPSS at 0, 25, 50, 100 and 200 μg/ml on the proliferation of K562 cells at 24, 48 and 72 hours. Flow cytometry was used to analyze the changes of cell cycle and apoptosis at 24 and 48 hours. Wright-Giemsa staining was used to observe the morphological changes of K562 cells. The cell surface antigens CD33 and CD11b were detected by flow cytometry.
RESULTS:
The proliferation of K562 cells treated with different concentrations of DPSS was inhibited in a time-dose dependent manner (r=-0.96). Cell cycle analysis showed that with the increase of DPSS concentration, cells in G2/M phase increased (r=0.88), and cells were blocked in G2/M phase. Flow cytometry results showed that with the apoptosis rate of K562 cells was the highest when treated with 200 μg/ml DPSS for 48 h. Morphological observation showed that the K562 cell body increased, the amount of cytoplasm increased, the ratio of nucleus to cytoplasm decreased, and the nuclear chromatin was rough after DPSS treatment. Cell differentiation antigen, CD33 and CD11b, were positively expressed after treated with DPSS.
CONCLUSION
DPSS can induce apoptosis through cell cycle arrest, inhibit the proliferation of K562 cells, and induce K562 cells to differentiate into monocytes, which has a potential anti-leukemia effect.
Humans
;
K562 Cells
;
Patrinia
;
Methylene Chloride/pharmacology*
;
Apoptosis
;
Cell Proliferation
;
Cell Differentiation