Osthole, the coumarin isolated from the fruits of Cnidum monnieri (L.) Cusson. The effects of osthole on the physiological properties in isolated auricle of guinea pig were studied. It possesses dose—dependent negative inotropic effects, the BaCl_2 inducing automaticity was inhibited, and the functional refractory period (FRP) was prolonged, and the frequence of sinoatrial node was slowed The negative inotropism and automatism suggest that sothole mey be antagonis tic to Ca~(2+), while the decrease of excitability and the prolongation of FRP indicate that it may be due to its inhibition on Na~+ current. The decrease of automaticity and excitability and prolongation of FRP are thought to be its antiarrhythmic action.

An tiinflammatory effects of alcohol extract of Caesalpinia sepiarla Roxb. var. pubescent Tang et wang were shown by ip iujection. The extract significantly decreased xylene induced swelling of mice ear; carrageenin produced edema of hind paw in rats, and decreased volume of pleural exudate on carrageenin-induced acute pleurisy of rats. It also inhibited egg white induced arthritis iu rats.LD50 value is 303.7mg/kg by ip in mice, confidence limit of 95% is 296.5 ? 189.4mg/kg.

It was reported that Os-thole(Ost) was isolated from the fruits of Cnidium monnieri(L. ) Cusson, possessesanti - arrhythmic action and depressed the aortic pressure action in animal studies. Its calcium -antagonistic effects were studied on the isolated rabbit aortic strips.Ost shifted the dose response curves for NE, CaCl2 and KC1 nonparallelly to the right, and depressed their maximal response; pD2' value is 3. 16, 4. 28 and 4. 39 respectively. It produced neither ?- a-drenoceptic stimulating nor ? - adreno-ceptic blocking effects. At the concentration of 100 ?mol ? L-1, Ost significantly inhibited the intra cellular Ca2+ dependent component of NE - induced contraction of the aortic strips, however, it didn't in-hibit the extra cellular Ca2+ dependent component of NE - induced contraction of the aortic strips, These results indicated that Ost produced relaxation on vascular smooth muscle might result from its antagonistic effect to Ca2+ and mainly blocked calcium influx through PDC, and the mode of its anta -gonism is similar to that of verapamil.

Adolescent ; Adult ; Child ; Congenital Abnormalities ; surgery ; Congenital Microtia ; Ear ; abnormalities ; surgery ; Female ; Humans ; Male ; Reconstructive Surgical Procedures ; Treatment Outcome ; Young Adult

Objective:To observe the clinical therapeutic effect of needling the acupoints of Governor Vessel and Bladder Meridian plus cupping on chronic fatigue syndrome. Methods: Fifty cases of chronic fatigue syndrome were divided into the treatment group(n= 25) and the control group(n=25). The patients in the treatment group were treated by needling Dazhui (GV 14), Zhiyang (GV 9),Xinshu (BL 15), Geshu (BL 17), Mingmen (GV 4), Shenshu (BL 23) and Changqiang (GV 1). Even reinforcing and reducing manipulation was used. Every time after the needles were withdrawn, the glass jar with thick and smooth mouth was selected to go on the back. The patients in the control group were treated by taking Chinese medicinal herbs, Caili composition, orally. Results: After 3durations of treatment, the therapeutic effects of the two groups were 92. 0% and 64. 0%respectively. There was significant difference in the therapeutic effect between the two groups(x2 =7.29,P ＜ 0.05).Conclusion: Acupuncture plus cupping is an effective method in treating chronic fatigue syndrome.

Objective To explore the effect for nerve regeneration with immunosuppresssant FK506 a-round the cut after end-to-side neurorrhaphy of peripheral nerve. Methods There were 40 adult SD rats and were divided into two groups randomly by means. In the experiment group, the right peroneal nerve of SD rat was cut off,then an 1mm epineural window was created on the neighboring tibial nerve,the distal end of peroneal nerve was sutured to the windowed tibial nerve by means of end-to-side attachment, application of slow-releasing diaphragm which was implanted around the nerve anastomosis with FK506. In the contrast group,there were not the FK506 around the cut after end-to-side neurorrhaphy. The tibial and peroneal nerve trunks were labeled by being in jected with fast blue ( KB) and fluorescence golden ( FG) respectively. The labeled cells in the dorsal root ganglia (DRG) and spinal cord were observed by fluorescence microscopy. Results There were a lot of FB labeled cells in the experiment group than in the contrast group in the DRG and spinal cord. Conclusion Immunosuppresssant FK506 could promote nerve regenerative speed and quality after end-to-side neurorrhaphy of peripheral nerve.

BackgroundWith the increasing prevalence of dry eye and the continuous improvement of living standards,the problem of dry eye more and more get the attention of people.At present,China still lacks the large population-based epidemiological data of dry eye. Objective To investigate the prevalence and possible risk factors of dry eye in a community of Huidong County of population aged 14 and over.Methods From September 2010 to January 2011,using questionnaires and examination of dry eye related,2800 people were selected randomly for cross-sectional survey.Those suspected as dry eye were examed by the SchirmerⅠtest ( S Ⅱ T),tear-film breakup time(BUT),corneal fluorescein staining(F1).Results In the 2475 questionnaire effectively,154 persons were diagnosed as dry eye,and the prevalence rate of dry eye was 6.22％,8.06％in females,4.14％in males.The prevalence rate increases with age.The S Ⅰ T and BUT decreased with increasing age.S Ⅰ T and BUT in females are less than males.Foreign body sensation is the primary complaints of patients.Logistic analysis showed that the most common risk factors in dry eye are age and gender.The system disease and eye diseases,eye fatigue and long exposure to dust are also main determinants.ConclusionsThe population prevalence rate of dry eye increased with age,the prevalence rate of dry eye in females is higher than that in males.The key factors associated with dry eye are age,gender,systemic disease and eye diseases,occupation,working environment.

OBJECTIVETo study the effect of Q-switched 1 064 nm Nd:YAG laser treatment on the proliferation of dermal collagen and expression of immunoglobulin binding protein/glucose related protein 78 (BiP/GRP78) in rats' skin and the mechanism of endoplasmic reticulum stress.

METHODSDorsal skin of 25 Wistar rats was divided into two parts equally after hair removal. Q-switched 1 064nm Nd:YAG laser was applied to treat rats' dorsal skin for 4 times at an interval of 2 days in the experiment part. The control part received no laser treatments. The rats' dorsal skin samples were taken on the 14th and 30th day after laser treatment to measure the dermis thickness and collagen bundles under HE stain and to measure the hydroxyproline content by alkaline hydrolysis method after laser treatment. The expression of BiP/GRP78 was also detected by immunohistochemical method. Statistics was used to analyze the data.

RESULTSThe dermis thickness increased by 29. 6% on the 14th day and 16.7% on the 30th day after laser treatment. The collagen bundles became thicker and denser. The hydroxyproline in the skin was also raised after laser treatment (P < 0.05). The immunohistochemical result showed the expression of BiP/GRP78 increased to 100% after laser treatment, showing a significant difference from the control group(X2 = 28.76, P < 0.01).

CONCLUSIONSThe Q-switched 1064 nm Nd:YAG laser treatment can induce endoplasmic reticulum stress, so as to enhance the protein folding and synthesizing precisely. The proliferation of dermis collagen is the base of effect of non-ablative skin rejuvenation.

Animals ; Endoplasmic Reticulum Stress ; Female ; Hydroxyproline ; chemistry ; Lasers, Solid-State ; Rats ; Rats, Wistar ; Rejuvenation ; Skin ; radiation effects ; Skin Aging

Objective To explore the replantation methods of the amputated tisue mass of fingers. Methods Fifteen cases were replanted using the physiological blood circulation replantation and the no physi- ological blood circulation replantation.Results All eleven cases survived with the physiological blood circu- lation replantation,one case failure with no physiological blood circulation replantation.Postoperative follow up ranged from six months to two years,with an average of fifteen months,the function and appearance were satis- factory.According to Hand Surgery of Chinese Medical Association' s functional evaluation in digital replanta- tion,eleven cases were excellent and two cases were good,the excellent and good rates were up to 86.7%. Conclusion For the amputated tissue mass of fingers,the physiological blood circulation replantation is the best choose.

DNA, Catalytic ; genetics ; Gene Expression ; Genetic Therapy ; Hep G2 Cells ; Hepacivirus ; genetics ; Humans ; Ribosomes ; genetics ; Transfection