The study aims to identify the rate of pregnancy patients in childbearing-age female who have biliary ascariasis. Fetal abnormalities have not found. Complications as biliary infection, acute pancreatitis and pancreatic reactions were observed.
Ascariasis ; pregnancy ; pregnant women

A study on 126 patients with biliary ascariasis (female/male: 4/1) in December 1997 in Hue Central Hospital has shown that the disease is initiated suddenly with severe pain in the right subcostal area and right chest (most common), vomiting, icterus, history of disease (80%). The common complications are bile duct infection, acute gallbladder infection, pancreatic reactions, acute pancreatitis, bile duct haemorrhage, bile duct abscess. Drugs: Pyrantil, antibiotics.
Ascariasis ; diagnosis ; therapy ; therapeutics

Background: It is important to diagnosis and properly treat patients with diarrhea is having a highly sensitive and specific technique to rapidly identify the caused bacteria, especially Diarrheagenic Escherichia Coli (DEC) isolates. Objectives: To develop and complete the bacterial DNA extraction procedure and optimize the DNA concentration for multiplex PCR for DEC. Materials and method: 7 reference strains of DEC and 10 fecal samples taken randomly were tested using DNA extraction and PCR techniques. Results. A bacterial DNA extraction procedure has been developed and optimized. This is a simple process and does not require expensive equipment. The test result is available after 90 \u2013 100 minutes. The minimum DNA content required for PCR to give positive results is 100ng per reaction. Conclusion: The development and completion of DNA extraction procedure plays an important role in early detection of DEC in fecal samples and serves as a base for further research on diarrheagenic bacteria.
DNA extraction ; PCR ; Escherichia coli

As the associations of sperm DNA fragmentation with morphology have not been examined in detail, this study aimed to investigate the relationship between abnormalities of morphological details and DNA integrity in human sperm. Methods: In this cross-sectional study, men from infertile couples were enrolled at Hue Center for Reproductive Endocrinology and Infertility, Vietnam. Conventional semen parameters, including morphological details, were analyzed following the World Health Organization 2010 criteria. Sperm DNA fragmentation was evaluated using a sperm chromatin dispersion assay. The relationships and correlations between semen parameters, sperm morphology, and the type of halosperm and the DNA fragmentation index (DFI) were analyzed. Results: Among 130 men in infertile couples, statistically significant differences were not found in the sperm halo type between the normal and abnormal sperm morphology groups. The percentage of round-head spermatozoa was higher in the DFI >15% group (16.98%±12.50%) than in the DFI ≤15% group (13.13% ±8.82%), higher values for amorphous heads were found in the DFI >15% group, and lower values for tapered heads were observed in the DFI ≤15% group; however, these differences were not statistically significant. Small-halo sperm and the DFI were positively correlated with round-head sperm (r=0.243, p=0.005 and r=0.197, p=0.025, respectively). Conclusion: The rate of general sperm morphological abnormalities in semen analysis was not related to sperm DNA integrity. However, round sperm heads were closely associated with sperm DNA fragmentation.

Objective: This study aimed to compare the efficacy of physiological intracytoplasmic sperm injection (PICSI) and intracytoplasmic sperm injection (ICSI) in terms of the fertilization rate and embryo quality using sibling oocyte cycles. Methods: This prospective, cross-sectional study collected data from 76 couples who underwent their first cycle at the Hue Center for Reproductive Endocrinology and Infertility, Vietnam, between May 2019 and November 2021. The inclusion criteria were cycles with at least eight oocytes and a sperm concentration of 5×106/mL. Sperm parameters, sperm DNA fragmentation (SDF), fertilization, and the quality of cleavage-stage embryos on day 2 and blastocysts on day 5 were examined. Results: From 76 ICSI cycles, 1,196 metaphase II (MII) oocytes were retrieved, half of which were randomly allocated to either the PICSI (n=592) or ICSI (n=604) treatment group. The results showed no significant difference between the two groups in terms of fertilization (72.80% vs. 75.33%, p=0.32), day 2 cleavage rate (95.13% vs. 96.04%, p=0.51), blastulation rate (52.68% vs. 57.89%), and high-quality blastocyst rate (26.10% vs. 31.13%, p=0.13). However, in cases where SDF was low, 59 cycles consisting of 913 MII oocytes produced a considerably higher blastulation rate with PICSI than with ICSI (50.49% vs. 35.65%, p=0.00). There were no significant differences between the pregnancy outcomes of the PICSI and ICSI embryo groups following embryo transfer. Conclusion Using variable sperm quality provided no benefit for PICSI versus ICSI in terms of embryo outcomes. When SDF is low, PICSI appears to be able to produce more blastocysts.