Using human rotavirus strain VNHR203-027 to inoculate and passage 11 consecutive times on Africa green monkey kidney (Vero) cells with inoculum medium at different trypsine concentrations, their results are VNHR203-027 have been adapting and growing on Vero cells with trypsin concentration of 30g/ml. Using human rotavirus strain VNHR203-037 to multiply on 4 lots of primary monkey kidney cells and 6 lots of Vero cells gave very high titre on both kinds of cell, the average titre on primary monkey kidney cells was 8,47 Iog10 FFU/ml and of Vero cells was 8.65 Iog10 FFU/ml. No significant difference in potency is found among Vero cells and primary monkey kidney cells (T=10-2 < t=1.86 with =0.05).
Rotavirus ; Cell Culture Techniques