Nucleotide analysis of restricted fragment DNA extremities at definite cut sizes identified: there were 3 extremities: flat; cross with DNA 5’; cross with DNA 5’. This study presented a method which able to characterize unknown restriction enzymes by analyzing the cutting extremities of restricted fragments. The method is based on Sanger sequencing of DNA around the cut site and non-radioactive chemical. Klenow reaction had two active characters: analyzing DNA 5’3’ and exonucleasa 3’5’, allowing to determine the type of cutting of restrictive enzymes. This results allowed to identify the cut sizes of two new enzymes: for Sml1, the cut sites occurs within the recognition nucleotide sequence; BciVI recognizing a non-palindrome sequence and cuts outside this sequence.
DNA ; Nucleotides ; analysis ; extremities ; Enzymes

A study was performed to identify a high activity restriction enzyme recognizing 5’-GCGC-3’. Isoschizomer Hhal is a endonucleaza, which has been found in a bacteria isolated from a soil sample picked up in Nghean province. This enzyme has a similar activity with Hha l but it is a other protein of other host bacteria, therefore, it is temporary called a isoschizomer of Hhal. About the enzyme’s specific, Hhal cleaves 5 standard DNA molecules at 384 sites, in which 215 sites at DNA , 103 sites at DNA T7, 18 sites at x174, 31 sites at pBR 322 and 17 sites at pUC 19. Isoschizomer Hhal with high activity, stable met the need of users and laboratory of Vietnam.
Enzymes ; Bacteria

A rapid agglutination – inhibition test was realized with hCG-coated sheep erythrocytes and rabbit antibodies to hCG. Determined amount of hCG on the red blood cells and titrated concentration of antibodies allowed the test to be sensitive at 200 UI/l for confirmation of pregnancy at 2-3 weeks (0-5 days following the first missed period). 3.000 tests were implemented in Nha Trang Pasteur Institute and Hanoi Newborn & Mother Protection Hospital showed that confident rate was 99%. The test was then used for diagnosing the urines of women just before their abortion. The results indicated that more than 11% of these women, at less than 15 days following the first their abortion, were not pregnant and should avoid abortion.
Pregnancy Tests ; Early Diagnosis

An IS 6110 fingerprint analysis of 168 Mycobacterium tuberculosis strains isolated from Vietnamese patients has been performed; 67 strains are isolated in the North (N strains), strains isolated in the South comprise 66 strains from tuberculosis patients without AIDS (S strains) and 35 strains from tuberculosis patients with AIDS (A strains). The number of IS 6110 copies per strain varies from 1 to 22 copies. The population of N strains are different from the population of the overall South strains where there was a high prevalence of strains with more than 15 IS copies. That regional difference between the Vietnamese strains was clearly shown by the fingerprint patterns of strains with more than 6 IS. The populations S strains and the A strains have similar fingerprint patterns and 80% of the South strains belong to one big family. In the contrary, a high polymorphism was seen among the N strains
Tuberculosis ; Mycobacterium ; Sprains and Strains ; epedemiology

Background: Bacterial vaginosis is a very common vaginal infection. It occurs when bacteria that normally exist in small numbers in the vagina multiply rapidly. With the purpose of early diagnosis and timely treatment infection of vaginalis tract. Objectives: The study was conducted with 2 purposes: Firstly, identification of bacterial and fungical causa is of the vaginalis. Secondly, comparison of the cause among 2 groups of women with clinical symptoms and group came for health examination. Subjects and method:A cross-sectional study was carried out in sampled groups: 325 women with ginecological symptom and 385 women came for health examination at the clinic of the Institute of mother and Newborn protection. Both 2 groups were clinical examined and microbiological testing. Results: The results were showed: The prevalence of vaginalis of the group with ginecological symptoms was found 76.92% and in the other group was 56.1%. The was not any disparity in clinical and testing results. The microbiological causes were found: 22.15% candida in group with clinical symptom and 18.96% in the other group. Streptococcal group D was taken second place 20.61% in group with clinical symptom and 13.25%in group with health examination and folioing are S. aureus, E.coli, C.trachomatis, G.vaginalis. Conclusion: There\u2019s no significant difference in the prevalence of infection of vaginalis tract between some age groups in this study.
Sexually Transmitted Diseases ; Bacterial/ pathology Female