23 patients with mitral stenosid with or without mild mitral open, ages of 23-50 (male: 10) received an operation. As the results of direct measurement of pulmonary arterial pressure and left atrial pressure in an operation have shown that the pulmonary arterial pressure was direct correlated before and after operation(r= 0.572 before operation; r=0.65 after operation).These parameters were reduced significantly and rapidly after operation when comparing with these before operation.
Evaluation Studies ; Thoracic Surgery ; Mitral Valve Stenosis

23 patients (10 males, 13 females) with mitral stenosis aged from 23-50 years of old (mean 37.5 years) were enrolled in the study. Valvular opening area ranged from 0.7cm2 to 1.2cm2. 5 patients have mitral stenosis alone. 18 patients have mitral stenosis with insufficiency grade I, II. 14 patients have sinus rhythm and 98 patients have atrial fibrillation. Pulmonary artery pressure ranged 25mmHg to 45mmHg when measuring directly. There was closed positive correlation between pulmonary artery pressure and right ventricular pressure with r= 0.509 for pre-operation and 0.548 for post-operation. After valve detachment, both pulmonary artery pressure and right ventricular pressure decreased rapidly and dramatically in comparison with prior detachment with p = 0.01.
Mitral Valve Stenosis ; Ventricular Pressure

A study was carried out on the 23 patients (male:10, female: 13) with the mitral stenosid with merely close or combination with the mild mitral open (grade III), ages of 23-50 indicated an operation for seperation of close valve in the Army Central hospital 108 during 1/1998 to 10/1999 in which there were 5 patients with the mitral stenosid, 18 patients with the mitral stenosid in combination with the mitral open grade I, II, 14 patients with the atrial rhythm, 9 patients with atrial fibrillation. The atrial pressure reduced from 15-67 mmHg to 15-45 mmHg. There was a direct proportional between the left atrial and right ventricular pressures
Mitral Valve Stenosis ; Ventricular Pressure ; surgery

Background: Salmonella typhi (S.typhi) is the major cause of human typhoid fever outbreaks. In fact, there were various typhoid fever outbreaks that occurred in China, and India that was caused by S.typhi strain without Vi antigen. Objective: To determine whether the S.typhi strains with mutation of gene encoding Vi antigen exists in Vietnam and the rate of mutation (if they exists). Subject and methods: 450 S.typhi isolates were collected in the Northern, Central and Southern Region of Vietnam during 1995 and 2005. The isolates were analyzed by the PCR method in order to detect mutants by using 2 primer pairs of tviB and DE. Results and Conclusion: There was no clear evidence on the relationship between the widely used Typhi Vi vaccine in Vietnam and the existence and spread of the mutation of gene encoding Vi antigen of S.typhi. 30 out of 450 isolates mutated losing the gene encoding of Vi antigen, making it 6.67%. These isolates were spread out between 1995 and 2005 throughout the Northern, Central and Southern Regions of Vietnam, with a peak in 1999. A noteworthy point was the rate of mutation of S.typhi losing the gene encoding of Vi antigen in Vietnam during the period of study. However, the mutation rate of S.typhi in Vietnam was still higher than the ratio of similar mutations being published in the other countries worldwide and higher than the recommended level of the World Health Organization.
gene mutation ; Salmonella typhi

Background: A/H5N1 influenza virus spreads from birds to humans and cause influenza diseases with high mortality rate. Vaccination is the most effective way to protect communities from pandemic, reduce morbidity and mortality. The study of creating A/H5N1 influenza vaccines in conformity with Vietnam was the urgent need. Institute of Vaccine\u2019s Achievement (IVAC) studied production of inactivated influenza vaccine for human on egg-grown from reassortants NIBRG-14. Objectives: In order to produce experimentally A/H5N1 influenza vaccine for human in accordance with WHO requirements and set up a viable process for production of the vaccines. Subjects and method: 10 days embryonated eggs and NIBRG-14 strains were served to the study with LAL method to check endotoxin, Kijehdal method to test total protein. Results: IVAC had produced successfully 5 lots of absorbed vaccine A/H5N1 (FLUVAC) using NIBRG-14 strains and embryonated eggs. Initially, production and quality control processes had been set up at IVAC by applying the recommendations of WHO. Conclusion: The success of the study was a basis of the approval of the government to establish a influenza vaccine manufacturing facilities.
Influenza A Virus ; H5N1 Subtype ; Influenza Vaccines ; Humans ; Eggs

Papiliotrema huenov was previously reported to be highly tolerant of a range of extremely toxic heavy metals. This study aimed to identify the potential of P. huenov to remove manganese and copper from aqueous solution. Physical conditions which affect removal of Mn(II) and Cu(II) were determined. Optimal temperature for adsorption of both metal ions was 30°C, and optimal pH for maximum uptake of Mn(II) and Cu(II) were 5 and 6, respectively. Under these conditions, living cells of P. huenov accumulated up to 75.58% of 110 mg/ L Mn(II) and 70.5% of 128 mg/L Cu(II) over 120 h, whereas, the removal efficiency of metal ions by dead cells over 1 h was 60.3% and 56.5%, respectively. These results indicate that living cells are more effective than dead biomass for bioremediation, but that greater time is required. The experimental data extends the potential use of P. huenov in biosorption and bioaccumulation of toxic heavy metals to copper and manganese, two of the most common industrial contaminants.

Background: Fungal keratitis is a serious ocular infection that can cause corneal scarring and blindness. Currently, diagnosis of fungal pathogens remains a difficult problem. Objectives: To investigate the application of semi-nested PCR targeted ITS genes for detection of fungal agents causing keratitis. Material and method: Ten identified fungal strains, 4 bacterial strains, 20 scraping samples from patients with suspected fungal keratitis and 2 scraping samples from patients with suspected bacterial keratitis were tested using semi-nested PCR. Results: Semi-nested PCR showed positive results for the samples of identified fungal strains and for the 20 scraping samples from patients with suspected fungal keratitis. Neither samples of bacterial strains nor scraping samples from suspected bacterial keratitis patients gave positive PCR results. Conclusion: Semi-nested PCR is a robust tool for specific and rapid detection of fungal agents causing keratitis.
Fungal keratitis ; semi-nested PCR

Background: In Vietnam, there are a number of studies on the application of ATRA in treating acute promyelocytic leukemia (AML \u2013 M3) but they have still faced with certain difficulties. Objectives: (1). Study PML/RAR alpha fusion gene on the patients diagnosed with AML \u2013 M3. (2). Study the index of hematology of the PML/RAR alpha positive group. Subject and Method: 21 patients with acute promyelocytic leukemia (M3) were studied. All patients were examined with morphology, coagulation and cytogenetic tests and RNA were extracted from leukemic cells and PCR for PML/RAR alpha fusion transcript. Result and conclusion: PML/RAR alpha positive in 67% including 4 patients which were not discovered t(15; 17) by cytogenetic technique. Rates of three subtype (bcr1, bcr2 and bcr3) of PML/RAR alpha were 7 patients (50%), 3 patients (21,5%) and 4 patients (28,5%), respectively. WBC and bone marrow cells of PML/RAR alpha positive group were 5.08+/-3.87 and 155.82+/-106.21. D \u2013 Dimer level was 1954.89+/-1575.28; 93% of patients in the PML/RAR alpha positive group had DIC.
Acute promyelocytic leukemia ; M3 ; PML/RAR alpha

Background: Chromosome mutation type t(8;21) has quite a high frequency in acute myelogenous leukemia, which accounted for about 15% among adult patients. From 2001, the WHO has a new classification for acute myelogenous leukemia based on genetic mutations. Form had AML1/ETO were arranged into genetic mutation group with better prognosis and ability to fully recover after chemotherapy with a high dose of cytarabin. Objective: Study AML1/ETO fusion gene on the patients diagnosed with Acute Myelogenous Leukemia (AML), as well as the clinical features and some haematologic parameters of the AML1/ETO positive group. Subject and methods: 76 patients with AML were treating in the National Institute of Hematology & Blood Transfusion and the Department of Hematology & Blood Transfusion of Bach Mai Hospital from April 2007 to July 2008. These patients were studied for clinical examination, morphology and RNA were extracted from leukemic cells and PCR for AML1/ETO fusion transcript was performed. Results and conclusions: The incidence of AML1/ETO positive in the AML patients was 24%. The incidence of AML1/ETO positive in AML-M2 was 28%. In the AML1/ETO positive group: median age was 26.94+/-9.22; rate of severe anemia, hemorrhage, fever, infection, hepatomegaly, splenomegaly, lymphadenopathy and gum hypertrophy was 44%, 33%, 28%, 11%, 44%, 28%, 17% and 6%, respectively. Median hemoglobin, WBC, platelet, bone marrow cell count, % blast in peripheral blood and in bone marrow was 84.41+/-28.97 g/l, 29.42+/-31.36 g/l, 42.12+/-33.83 g/l, 215.93+/-134.42 g/l, 56.21+/-26.58% and 65.14+/-16.12%, respectively.
acute myelogenous leukemia ; AML1/ETO fusion gene

As the associations of sperm DNA fragmentation with morphology have not been examined in detail, this study aimed to investigate the relationship between abnormalities of morphological details and DNA integrity in human sperm. Methods: In this cross-sectional study, men from infertile couples were enrolled at Hue Center for Reproductive Endocrinology and Infertility, Vietnam. Conventional semen parameters, including morphological details, were analyzed following the World Health Organization 2010 criteria. Sperm DNA fragmentation was evaluated using a sperm chromatin dispersion assay. The relationships and correlations between semen parameters, sperm morphology, and the type of halosperm and the DNA fragmentation index (DFI) were analyzed. Results: Among 130 men in infertile couples, statistically significant differences were not found in the sperm halo type between the normal and abnormal sperm morphology groups. The percentage of round-head spermatozoa was higher in the DFI >15% group (16.98%±12.50%) than in the DFI ≤15% group (13.13% ±8.82%), higher values for amorphous heads were found in the DFI >15% group, and lower values for tapered heads were observed in the DFI ≤15% group; however, these differences were not statistically significant. Small-halo sperm and the DFI were positively correlated with round-head sperm (r=0.243, p=0.005 and r=0.197, p=0.025, respectively). Conclusion: The rate of general sperm morphological abnormalities in semen analysis was not related to sperm DNA integrity. However, round sperm heads were closely associated with sperm DNA fragmentation.