Objective: To examine the expression of P-gp,GST-?,TOPO? and Ki67 in lung cancer and study the clinical relationship of P-gp,GST-?,TOPO?and Ki67 Co-expression in lung cancer.Methods: The expression of P-gp,GST-?,TOPO? in 52 cases of lung cancer without chemotherapy was detected before operation by immunohistochemical staning.Results: The positive rates of P-gp,GST-?,TOPO?,Ki67 were respectively 73%,80.5%,62.5%,23.7%.The P-gp,GST-? expression was correlated with the histology and differentiation,The expression of TOPO? was obviously lower in adenocarcinoma than in other typers of lung cancers;The coexpression rate of more than one MDR gene production appareatly exceeded individual expression rate.The expression of P-gp,GST-?,TOPO? was not correlated with the expression of Ki67.Conclusion: P-gp,GST-?,TOPO? can be expressed in different types of lung cancers and with different expression levels,They are all correlated with multidrug resistance of lung cancer,Thus,they can be regarded as a valuable indicators for clinical chemotherapy and for prognosis in lung cancer patients.

Objective:To investigate the adverse drug reactions ( ADR) caused by carbapenem antibiotics and discuss the influen-cing factors to provide reference for the rational use of carbapenems in clinics. Methods:The ADR caused by carbapenems from March 1, 2008 to August 1, 2014 in our hospital were statistically analyzed. Results:Totally 73 cases of ADR were caused by carbapenems. The number of ADR for men and women was similar. The ADR occurred in 80-year-old people with more frequency. The incidence of ADR on the first day of administration was relative high. The ADR were mainly manifested as skin and appendages disorders and nerve system damage. Conclusion: Great attention should be paid to the ADR of carbapenems and the state of patients. The medication should be adjusted in the patients with declined renal function and nerve system basic diseases in order to reduce the damage of ADR.

Adult ; Aged ; Case-Control Studies ; Female ; Humans ; Leukemia, Myeloid, Acute ; metabolism ; pathology ; Male ; Middle Aged ; NF-kappa B ; metabolism ; Neovascularization, Pathologic ; pathology ; Tryptases ; metabolism ; Vascular Endothelial Growth Factor A ; metabolism ; Young Adult

Objective To investigate the predictive value of trauma index on the prognosis in pa-tients with traumatic spinal cord injury.Methods Enrolled 168 cases of patients with traumatic spinal cord injury,the traumatic index score and the American Spinal Injury Association (ASIA)grade were as-sessed on admission,then followed up for 6 months,the Spearman correlation analysis was performed to a-nalysis the relationship between trauma index and ASIA grade,and Receiver operating curve (ROC)anal-ysis was used to evaluate the relationship between trauma index and prognosis.Results There was a sig-nificant negative correlation between trauma index score and ASIA grade(r =-0.68,P <0.01).A total of 157 cases (93.5%)completed follow-up,the trauma index score had a good predictive value on the traumatic death in these patients,it's sensitivity was 71.4%,specificity was 76.1%,the best cut-off point was 25 points(P <0.01 );Compared with the low score group (<25 points),the high group (≥25 points)showed a lower ASIA grade and higher mortality,the differences were statistically significant (P <0.01).Conclusion The trauma index score was related with the spinal cord injury,and it is a predictive factor of poor prognosis in patients with traumatic spinal cord injury.

Bdellovibrio bacteria BDF-H16 was isolated from the gut of Carassius auratus gibelio with Aeromonas sobria.Its shape was ob- served by light microscopy,phase-contrast microscopy,electron microscopy and some of its biological characteristics were also studied.It was demonstrated that BDF-H16 was an gram-negative bacterium and had a bacilliform or arc bacilliform shape with a flagellum at one end.Its size was mostly 0.2?m～0.5?m?0.8?m～1.2?m.It had a wide prey area and could lyse all tested gram-negative bacteria and some gram-positive bacteria.The best lysis conditions to Escherichia coli were 6.75?10~9 cfu/mL of prey bacteria concentration,pH7.0～7.5,28℃.It could grow in the solid culture added 0.85%～5.00% NaCl and was inhibited by enrofloxacin and norfloxacin.

Objective To investigate the expression of cyclooxygenase-2(COX-2)and Survivin in ameloblastoma (AB) tissues. Methods A total of 60 AB samples (primary AB 40 cases, recurrent AB 20 cases) and 60 normal oral mucosas were collected from the Affiliated Hospital of Chengde Medical College. The AB samples included 12 acanthomatous types, 18 follicular patterns, 18 plexiform patterns, 6 basal cell ameloblastomas and 6 desmoplastic ameloblastomas. The expres?sion levels of COX-2 and Survivin were detected by immunohistochemistry and Western blot methods in AB and normal oral mucosa. The expressions of COX-2 and Survivin were compared between different types and tissues of AB. Results The positive rate of COX-2 was significantly higher in AB (82.7%) than that in normal oral mucosa (10.0%). The positive rate of Survivin was significantly higher in AB (83.3%) than that in normal oral mucosa tissues (16.7%). The expression of COX-2 (0.781±0.142) was higher in AB than that of normal oral mucosa tissues (0.179±0.056). The expression of Survivin (0.447± 0.139) was significantly higher in AB than those in normal oral mucosa tissues (0.072±0.017). There was a positive correla?tion between the expression of COX-2 and Survivin (r=0.778,P<0.05). There were no significant differences in the positive expressions of COX-2 and Survivin between different types of AB (P>0.05). Conclusion COX-2 and Survivin were over-expressed in AB, which may be involved in the occurrence and development of AB.

AIM:To elucidate the molecular mechanisms of lipoic acid (LA) on redox regulation and digestive function in intestine of C57BL/6 mice fed high fat diet (HFD).METHODS:C57BL/6 mice were randomly assigned to one of three groups (n=8). The control group consumed an ordinary diet. The other two experimental groups were fed with a high fat diet,high fat plus 0.1% LA. After 6 weeks,the activities of digestive enzymes were examined. In order to evaluate the antioxidant status of the mice,superoxide dismutase (SOD),malondialdehyde (MDA),total antioxidant capacity (TAC) and reactive oxygen species (ROS) in intestinal homogenate were measured. To investigate the molecular mechanisms underlying the effects of LA,the gene expression profiles in intestine were examined using the GeneChip microarray system.RESULTS:A depressed antioxidant defense system,accompanied by digestive and absorptive function impairment,was observed in HFD-fed mice. These changes were partially restored in the LA-treated group. DNA microarray analysis of intestine showed that LA ingestion up-regulated the expression of genes related to free-radical scavenger enzymes,digestive enzymes and transporters.CONCLUSION:Treatment with LA improves redox homeostasis and the function of intestine in mice fed HFD. The mechanism may involve preventing oxidative stress by scavenging ROS directly and increasing those of free-radical scavenger enzymes gene expression indirectly.

· AIM:To determine whether the multifocal visual evoked potential (mfVEP) can be used as a clinical method to assess the patients with optic nerve disease.· METHODS: Fifteen patients with optic nerve disease were examined in this study. All patients underwent visual acuity examination, slit-lamp inspection, ophthalmoscopy, Goldmann perimeter, fundus fiuorescein angiography, visual field and mfVEP. Although these patients with unilateral optic nerve damage,data from both eyes were included in the analysis.· RESULTS:In all patients the visual fields defect was demonstrated on the mfVEP and showed good correspondence in location of the scotoma. However, we also found some slight difference between visual field and mfVEP. In some locations, when the local visual fields were normal, mfVEP showed that its amplitude reduced. In reverse, when the local mfVEP seemed normal, visual field showed abnormity.· CONCLUSION:Multifocal visual evoked potential could be used as a clinical diagnosis option in patients with optic nerve disease. Local monocular damage to the optic nerve can be measured by an interocular comparison of the mfVEP.

In the experiment, the production of plagues by Bdellovibrio bacteria in solid medium cultivation, the reproduction of Bdellovibrio bacteria in liquid medium cultivation and the attachment of Bdellovibrio bacteria to carrier were observed, which aimed to study the effects of enrofloxacin on the growth and at-tachment ability of Bdellovibrio bacteria BDF-H16. Results indicated that in solid medium cultivation, the production of plagues by Bdellovibrio bacteria BDF-H16 was inhibited by different concentrations (2 ?g/mL, 5 ?g/mL, 10 ?g/mL, 20 ?g/mL, 50 ?g/mL) of enrofloxacin and the inhibitory effects of enrofloxacin became stronger with the increase of the concentration of enrofloxacin. Similarly, in liquid medium cultivation, the reproduction of Bdellovibrio bacteria BDF-H16 was also obviously inhibited by different concentrations ofenrofloxacin and higher concentrations of enrofloxacin such as 10 ?g/mL, 20 ?g/mL, 50 ?g/mL had stronger inhibitory effects on the reproduction of BDF-H16. However, the growth tendency of Bdellovibrio bacteria BDF-H16 was not inhibited in 10 ?g/mL enrofloxacin. Additionally, when zeolite was added, enrofloxacin had also inhibitory effects on the numbers of Bdellovibrio bacteria BDF-H16 attached to zeolite. With the increase of the concentrations of enrofloxacin, the numbers of Bdellovibrio bacteria BDF-H16 attached to zeolite became smaller and smaller. However, the attachment rate of Bdellovibrio bacteria BDF-H16 to zeo-lite became higher under 2 ?g/mL-20 ?g/mL enrofloxacin. The results above showed that enrofloxacin had inhibitory effects on the plague production and reproduction of Bdellovibrio bacteria BDF-H16, but the at-tachment ability of Bdellovibrio bacteria BDF-H16 was strengthened in liquid medium cultivation with 2 ?g/mL-20 ?g/mL enrofloxacin and zeolite, and adding zeolite helped to reduce the adverse effects of en-rofloxacin on Bdellovibrio bacteria BDF-H16.