Establish DNA polymorphism methods (PCR/RFLP) with marker Bcl1 restriction enzyme on intron 18 for detecting mothers who carried an abnormal X chromosome with hemophilia and transfer to their sons. 4 of 9 families carried intron 18-related mutations of hemophilia A and these mothers transfered mutations to their sons to cause hemophilia patients
Hemophilia A ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length

Backgroud: Phacoemulsification with trifocal intraocular lens helps patients achieve clear vision at all distances, reponding their needs and desires. Therefore, we have studied to evaluate the effectiveness of a trifocal intraocular lens. Objectives: (1) To describe the clinical features of cataract patients. (2) To evaluate the outcomes of cataract treatment by phacoemulsification with a trifocal intraocular lens. Methods: A prospective interventional clinical study was conducted on 80 eyes of 68 cataract patients who underwent phacoemulsification with a trifocal intraocular lens at the Ophthalmology Center, Hue Central Hospital. Results: At the 3-month postoperative period, 65.0% of the operated eyes had distance vision of 20/25 or better, 52.5% had intermediate vision of 20/25 or better, and 57.5% had near vision of 20/25 or better. The patient satisfaction rate was 91.2%. Conclusion: Phacoemulsification with a trifocal intraocular lens provides high effectiveness in terms of visual acuity and patient satisfaction.

As part of our continued study on the chemical constituents of Willughbeia edulis stems, a new dimeric lignan named edulignan (1) was isolated from its EtOAc-soluble extract. Based on NMR spectroscopic interpretation, the planar structure of 1 has been suggested to have two 2-substituted 4-chromanone subunits with different stereochemical configurations. In addition, the MS/MS analysis of the products obtained by acidcatalyzed hydrolysis of 1 was supportive of its structure. Unfornatually, the new compound 1 did not show α-glucosidase inhibitory activity with an IC 50 value > 250 μM.

Objectives: Survey on erectile dysfunction in patients with lumbosacral disc herniation and evaluation of their clinical improvement after surgical treatment of spinal canal stenosis due to herniated disc. Materials and Methods: 35 patients with lumbosacral were treated with discectomy with posterior intervertebral fusion (PLIF) surgery from 10/2021 - 4/2022 at the Department of Neurology, Hue University of Medicine and Pharmacy Hospital. Patients were evaluated for erectile dysfunction and postoperative improvement using the IIEF-5, CSFQ-14, DASS-21, Oswestry scales. Results: The mean age was 48.49 ± 9.15 years old. 51.4% of patients had the most severe hernia at the L4-L5 disc, 28.6% L3-L4 and 20% L5-S1. 100% of patients with herniated disc have erectile dysfunction. Of which 40% had mild-moderate disorders, 34.3% had moderate disorders, 14.3% had severe disorders and 11.4% had mild disorders. The CSFQ-14 and DASS-21 scales were correlated with erectile dysfunction (p < 0.05). Surgery significantly improved pain (VAS score), activity limitation (ODI and JOA), degree of erectile dysfunction, changes in sexual function, depression and anxiety, stress of the patient 1 month after surgery (p < 0.05). Conclusions: Patients with lumbosacral disc herniation had a 100% erectile dysfunction rate with a mean IIEF-5 score of 11.69 ± 3.77. The CSFQ-14 and DASS-21 scores have a strong positive correlation with erectile dysfunction. Surgical treatment of spinal canal stenosis due to lumbosacral disc herniation improves the clinical status of herniated disc as well as improves erectile dysfunction in these patients.

The optimal condition for Moringa oleifera root barks extraction was determined using response surface methodology and Box-Behnken Design. The actual optimal condition of the factors was 65 o C, ethanol 60%, 40 (mL/g) liquid-to-solid ratio with 240 minutes extraction time. The enrichment of phenolic compounds sharply affected the antioxidant, and inhibitions of α-amylase enzyme, as well as, the anti-inflammatory effect of the extract from M. oleifera root barks. The extract in the optimal condition exhibited better 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and α-amylase inhibitory activities than those of positive controls.Also, the extract showed weak hydroxyl free radical scavenging and nitric oxide (NO) production inhibitory effects. These revealed a simple and promising method for the preparation of bioactive products from the root bark of M. oleifera.

Background: Buxus latistyla Gagnep. (Buxaceae) has been used as a remedy for treating malaria, haemoptysis, amoebic dysentery etc. However, its chemical constituents are still unknown. Thus, the aim of this study was to identify phytochemicals isolated from this plant. Materials and method: Aerial parts of B. latistyla were collected from Quang Tri province. Pure compounds were isolated using the combination of chromatographic methods. Structures of isolated compounds were identified by analyzing spectral data of nuclear magnetic resonance as well as by comparing with reported data. Results & Conclusion: Five triterpenes were isolated and identified including lupeol (1), lupenone (2), betulin (3), 20(29)-lupene-2α,3α-diol (4), 20(29)-lupene-2α,3α,28-triol (5). This is the first report about those phytochemicals of B. latistyla collected in Vietnam.